Construction of a map of chromosome 16 by using radiation hybrids

I. Ceccherini; G. Romeo; S. Lawrence; M. H. Breuning; P. C. Harris; H. Himmelbauer; A. M. Frischauf; G. R. Sutherland; G. G. Germino; S. T. Reeders; N. E. Morton

doi:10.1073/pnas.89.1.104

Construction of a map of chromosome 16 by using radiation hybrids

I. Ceccherini, G. Romeo, S. Lawrence, M. H. Breuning, P. C. Harris, H. Himmelbauer, A. M. Frischauf, G. R. Sutherland, G. G. Germino, S. T. Reeders, N. E. Morton

Nephrology and Hypertension

Research output: Contribution to journal › Article › peer-review

37 Scopus citations

Abstract

A human-hamster cell hybrid carrying a single copy of chromosome 16 as the only human genetic material was irradiated with a single dose of γ-rays (7000 rads; 1 rad = 0.01 Gy) and then fused with a thymidine kinase-deficient hamster cell line (RJKM) to generate radiation hybrids retaining unselected fragments of this human chromosome. In two experiments, 223 hybrids were isolated in hypoxanthine/aminopterine/thymidine (HAT) medium and screened with 38 DNA probes, corresponding to anonymous DNA or gene sequences localized on chromosome 16. The most likely order and location of the 38 DNA sequences were established by multiple pairwise analysis and scaled to estimate physical distance in megabases. The order and the distances thus obtained are mostly consistent with available data on genetic and physical mapping of these markers, illustrating the usefulness of radiation hybrids for mapping.

Original language	English (US)
Pages (from-to)	104-108
Number of pages	5
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	89
Issue number	1
DOIs	https://doi.org/10.1073/pnas.89.1.104
State	Published - 1992

Keywords

Multiple pairwise analysis
Physical maps
Somatic cell hybrids

ASJC Scopus subject areas

General

Access to Document

10.1073/pnas.89.1.104

Cite this

Ceccherini, I., Romeo, G., Lawrence, S., Breuning, M. H., Harris, P. C., Himmelbauer, H., Frischauf, A. M., Sutherland, G. R., Germino, G. G., Reeders, S. T., & Morton, N. E. (1992). Construction of a map of chromosome 16 by using radiation hybrids. Proceedings of the National Academy of Sciences of the United States of America, 89(1), 104-108. https://doi.org/10.1073/pnas.89.1.104

Ceccherini, I, Romeo, G, Lawrence, S, Breuning, MH, Harris, PC, Himmelbauer, H, Frischauf, AM, Sutherland, GR, Germino, GG, Reeders, ST & Morton, NE 1992, 'Construction of a map of chromosome 16 by using radiation hybrids', Proceedings of the National Academy of Sciences of the United States of America, vol. 89, no. 1, pp. 104-108. https://doi.org/10.1073/pnas.89.1.104

@article{caa4d1d442bc4577bb3e4176e5adfd80,

title = "Construction of a map of chromosome 16 by using radiation hybrids",

abstract = "A human-hamster cell hybrid carrying a single copy of chromosome 16 as the only human genetic material was irradiated with a single dose of γ-rays (7000 rads; 1 rad = 0.01 Gy) and then fused with a thymidine kinase-deficient hamster cell line (RJKM) to generate radiation hybrids retaining unselected fragments of this human chromosome. In two experiments, 223 hybrids were isolated in hypoxanthine/aminopterine/thymidine (HAT) medium and screened with 38 DNA probes, corresponding to anonymous DNA or gene sequences localized on chromosome 16. The most likely order and location of the 38 DNA sequences were established by multiple pairwise analysis and scaled to estimate physical distance in megabases. The order and the distances thus obtained are mostly consistent with available data on genetic and physical mapping of these markers, illustrating the usefulness of radiation hybrids for mapping.",

keywords = "Multiple pairwise analysis, Physical maps, Somatic cell hybrids",

author = "I. Ceccherini and G. Romeo and S. Lawrence and Breuning, {M. H.} and Harris, {P. C.} and H. Himmelbauer and Frischauf, {A. M.} and Sutherland, {G. R.} and Germino, {G. G.} and Reeders, {S. T.} and Morton, {N. E.}",

year = "1992",

doi = "10.1073/pnas.89.1.104",

language = "English (US)",

volume = "89",

pages = "104--108",

journal = "Proceedings of the National Academy of Sciences of the United States of America",

issn = "0027-8424",

publisher = "National Academy of Sciences",

number = "1",

}

TY - JOUR

T1 - Construction of a map of chromosome 16 by using radiation hybrids

AU - Ceccherini, I.

AU - Romeo, G.

AU - Lawrence, S.

AU - Breuning, M. H.

AU - Harris, P. C.

AU - Himmelbauer, H.

AU - Frischauf, A. M.

AU - Sutherland, G. R.

AU - Germino, G. G.

AU - Reeders, S. T.

AU - Morton, N. E.

PY - 1992

Y1 - 1992

N2 - A human-hamster cell hybrid carrying a single copy of chromosome 16 as the only human genetic material was irradiated with a single dose of γ-rays (7000 rads; 1 rad = 0.01 Gy) and then fused with a thymidine kinase-deficient hamster cell line (RJKM) to generate radiation hybrids retaining unselected fragments of this human chromosome. In two experiments, 223 hybrids were isolated in hypoxanthine/aminopterine/thymidine (HAT) medium and screened with 38 DNA probes, corresponding to anonymous DNA or gene sequences localized on chromosome 16. The most likely order and location of the 38 DNA sequences were established by multiple pairwise analysis and scaled to estimate physical distance in megabases. The order and the distances thus obtained are mostly consistent with available data on genetic and physical mapping of these markers, illustrating the usefulness of radiation hybrids for mapping.

AB - A human-hamster cell hybrid carrying a single copy of chromosome 16 as the only human genetic material was irradiated with a single dose of γ-rays (7000 rads; 1 rad = 0.01 Gy) and then fused with a thymidine kinase-deficient hamster cell line (RJKM) to generate radiation hybrids retaining unselected fragments of this human chromosome. In two experiments, 223 hybrids were isolated in hypoxanthine/aminopterine/thymidine (HAT) medium and screened with 38 DNA probes, corresponding to anonymous DNA or gene sequences localized on chromosome 16. The most likely order and location of the 38 DNA sequences were established by multiple pairwise analysis and scaled to estimate physical distance in megabases. The order and the distances thus obtained are mostly consistent with available data on genetic and physical mapping of these markers, illustrating the usefulness of radiation hybrids for mapping.

KW - Multiple pairwise analysis

KW - Physical maps

KW - Somatic cell hybrids

UR - http://www.scopus.com/inward/record.url?scp=0026601166&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026601166&partnerID=8YFLogxK

U2 - 10.1073/pnas.89.1.104

DO - 10.1073/pnas.89.1.104

M3 - Article

C2 - 1729675

AN - SCOPUS:0026601166

SN - 0027-8424

VL - 89

SP - 104

EP - 108

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

IS - 1

ER -

Construction of a map of chromosome 16 by using radiation hybrids

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this