Construction and testing of orfA +/- FIV reporter viruses

Hind Fadel, Dyana T. Saenz, Eric M. Poeschla

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Single cycle reporter viruses that preserve the majority of the HIV-1 genome, long terminal repeat-promoted transcription and Rev-dependent structural protein expression are useful for investigating the viral life cycle. Reporter viruses that encode the viral proteins in cis in this way have been lacking for feline immunodeficiency virus (FIV), where the field has used genetically minimized transfer vectors with viral proteins supplied in trans. Here we report construction and use of a panel of single cycle FIV reporter viruses that express fluorescent protein markers. The viruses can be produced to high titer using human cell transfection and can transduce diverse target cells. To illustrate utility, we tested versions that are (+) and (-) for OrfA, an FIV accessory protein required for replication in primary lymphocytes and previously implicated in down-regulation of the primary FIV entry receptor CD134. We observed CD134 down-regulation after infection with or without OrfA, and equivalent virion production as well. These results suggest a role for FIV proteins besides Env or OrfA in CD134 down-regulation.

Original languageEnglish (US)
Pages (from-to)184-199
Number of pages16
JournalViruses
Volume4
Issue number1
DOIs
StatePublished - Jan 2012

Fingerprint

Feline Immunodeficiency Virus
Viruses
Down-Regulation
Viral Proteins
env Gene Products
Virus Receptors
Virus Internalization
Proteins
Terminal Repeat Sequences
Life Cycle Stages
Virion
Transfection
HIV-1
Genome
Lymphocytes
Infection

Keywords

  • FIV
  • Lentivirus
  • OrfA
  • Reporter virus

ASJC Scopus subject areas

  • Infectious Diseases
  • Virology

Cite this

Construction and testing of orfA +/- FIV reporter viruses. / Fadel, Hind; Saenz, Dyana T.; Poeschla, Eric M.

In: Viruses, Vol. 4, No. 1, 01.2012, p. 184-199.

Research output: Contribution to journalArticle

Fadel, Hind ; Saenz, Dyana T. ; Poeschla, Eric M. / Construction and testing of orfA +/- FIV reporter viruses. In: Viruses. 2012 ; Vol. 4, No. 1. pp. 184-199.
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