TY - JOUR
T1 - Constipation-Predominant Irritable Bowel Syndrome Females Have Normal Colonic Barrier and Secretory Function
AU - Peters, Stephanie A.
AU - Edogawa, Shoko
AU - Sundt, Wendy J.
AU - Dyer, Roy B.
AU - Dalenberg, Daniel A.
AU - Mazzone, Amelia
AU - Singh, Ravinder Jit
AU - Moses, Natalie
AU - Smyrk, Thomas Christopher
AU - Weber, Christopher
AU - Linden, David R
AU - MacNaughton, Wallace K.
AU - Turner, Jerrold R.
AU - Camilleri, Michael
AU - Katzka, David A
AU - Farrugia, Gianrico
AU - Grover, Madhusudan
PY - 2017/3/21
Y1 - 2017/3/21
N2 - OBJECTIVES:The objective of this study was to determine whether constipation-predominant irritable bowel syndrome (IBS-C) is associated with changes in intestinal barrier and secretory function.METHODS:A total of 19 IBS-C patients and 18 healthy volunteers (all females) underwent saccharide excretion assay (0.1 g 13C mannitol and 1 g lactulose), measurements of duodenal and colonic mucosal barrier (transmucosal resistance (TMR), macromolecular and Escherichia coli Bio-Particle translocation), mucosal secretion (basal and acetylcholine (Ach)-evoked short-circuit current (Isc)), in vivo duodenal mucosal impedance, circulating endotoxins, and colonic tight junction gene expression.RESULTS:There were no differences in the in vivo measurements of barrier function between IBS-C patients and healthy controls: cumulative excretion of 13C mannitol (0–2 h mean (s.e.m.); IBS-C: 12.1 (0.9) mg vs. healthy: 13.2 (0.8) mg) and lactulose (8–24 h; IBS-C: 0.9 (0.5) mg vs. healthy: 0.5 (0.2) mg); duodenal impedance IBS-C: 729 (65) Ω vs. healthy: 706 (43) Ω; plasma mean endotoxin activity level IBS-C: 0.36 (0.03) vs. healthy: 0.35 (0.02); and in colonic mRNA expression of occludin, zonula occludens (ZO) 1–3, and claudins 1–12 and 14–19. The ex vivo findings were consistent, with no group differences: duodenal TMR (IBS-C: 28.2 (1.9) Ω cm2 vs. healthy: 29.8 (1.9) Ω cm2) and colonic TMR (IBS-C: 19.1 (1.1) Ω cm2 vs. healthy: 17.6 (1.7) Ω cm2); fluorescein isothiocyanate (FITC)–dextran (4 kDa) and E. coli Bio-Particle flux. Colonic basal Isc was similar, but duodenal basal Isc was lower in IBS-C (43.5 (4.5) μA cm2) vs. healthy (56.9 (4.9) μA cm2), P=0.05. Ach-evoked ΔIsc was similar.CONCLUSIONS:Females with IBS-C have normal colonic barrier and secretory function. Basal duodenal secretion is decreased in IBS-C.Am J Gastroenterol advance online publication, 21 March 2017; doi:10.1038/ajg.2017.48.
AB - OBJECTIVES:The objective of this study was to determine whether constipation-predominant irritable bowel syndrome (IBS-C) is associated with changes in intestinal barrier and secretory function.METHODS:A total of 19 IBS-C patients and 18 healthy volunteers (all females) underwent saccharide excretion assay (0.1 g 13C mannitol and 1 g lactulose), measurements of duodenal and colonic mucosal barrier (transmucosal resistance (TMR), macromolecular and Escherichia coli Bio-Particle translocation), mucosal secretion (basal and acetylcholine (Ach)-evoked short-circuit current (Isc)), in vivo duodenal mucosal impedance, circulating endotoxins, and colonic tight junction gene expression.RESULTS:There were no differences in the in vivo measurements of barrier function between IBS-C patients and healthy controls: cumulative excretion of 13C mannitol (0–2 h mean (s.e.m.); IBS-C: 12.1 (0.9) mg vs. healthy: 13.2 (0.8) mg) and lactulose (8–24 h; IBS-C: 0.9 (0.5) mg vs. healthy: 0.5 (0.2) mg); duodenal impedance IBS-C: 729 (65) Ω vs. healthy: 706 (43) Ω; plasma mean endotoxin activity level IBS-C: 0.36 (0.03) vs. healthy: 0.35 (0.02); and in colonic mRNA expression of occludin, zonula occludens (ZO) 1–3, and claudins 1–12 and 14–19. The ex vivo findings were consistent, with no group differences: duodenal TMR (IBS-C: 28.2 (1.9) Ω cm2 vs. healthy: 29.8 (1.9) Ω cm2) and colonic TMR (IBS-C: 19.1 (1.1) Ω cm2 vs. healthy: 17.6 (1.7) Ω cm2); fluorescein isothiocyanate (FITC)–dextran (4 kDa) and E. coli Bio-Particle flux. Colonic basal Isc was similar, but duodenal basal Isc was lower in IBS-C (43.5 (4.5) μA cm2) vs. healthy (56.9 (4.9) μA cm2), P=0.05. Ach-evoked ΔIsc was similar.CONCLUSIONS:Females with IBS-C have normal colonic barrier and secretory function. Basal duodenal secretion is decreased in IBS-C.Am J Gastroenterol advance online publication, 21 March 2017; doi:10.1038/ajg.2017.48.
UR - http://www.scopus.com/inward/record.url?scp=85015743578&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85015743578&partnerID=8YFLogxK
U2 - 10.1038/ajg.2017.48
DO - 10.1038/ajg.2017.48
M3 - Article
C2 - 28323272
AN - SCOPUS:85015743578
JO - American Journal of Gastroenterology
JF - American Journal of Gastroenterology
SN - 0002-9270
ER -