Constipation-Predominant Irritable Bowel Syndrome Females Have Normal Colonic Barrier and Secretory Function

Stephanie A. Peters, Shoko Edogawa, Wendy J. Sundt, Roy B. Dyer, Daniel A. Dalenberg, Amelia Mazzone, Ravinder Jit Singh, Natalie Moses, Thomas Christopher Smyrk, Christopher Weber, David R Linden, Wallace K. MacNaughton, Jerrold R. Turner, Michael Camilleri, David A Katzka, Gianrico Farrugia, Madhusudan Grover

Research output: Contribution to journal › Article

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Abstract

OBJECTIVES:The objective of this study was to determine whether constipation-predominant irritable bowel syndrome (IBS-C) is associated with changes in intestinal barrier and secretory function.METHODS:A total of 19 IBS-C patients and 18 healthy volunteers (all females) underwent saccharide excretion assay (0.1 g 13C mannitol and 1 g lactulose), measurements of duodenal and colonic mucosal barrier (transmucosal resistance (TMR), macromolecular and Escherichia coli Bio-Particle translocation), mucosal secretion (basal and acetylcholine (Ach)-evoked short-circuit current (Isc)), in vivo duodenal mucosal impedance, circulating endotoxins, and colonic tight junction gene expression.RESULTS:There were no differences in the in vivo measurements of barrier function between IBS-C patients and healthy controls: cumulative excretion of 13C mannitol (0–2 h mean (s.e.m.); IBS-C: 12.1 (0.9) mg vs. healthy: 13.2 (0.8) mg) and lactulose (8–24 h; IBS-C: 0.9 (0.5) mg vs. healthy: 0.5 (0.2) mg); duodenal impedance IBS-C: 729 (65) Ω vs. healthy: 706 (43) Ω; plasma mean endotoxin activity level IBS-C: 0.36 (0.03) vs. healthy: 0.35 (0.02); and in colonic mRNA expression of occludin, zonula occludens (ZO) 1–3, and claudins 1–12 and 14–19. The ex vivo findings were consistent, with no group differences: duodenal TMR (IBS-C: 28.2 (1.9) Ω cm2 vs. healthy: 29.8 (1.9) Ω cm2) and colonic TMR (IBS-C: 19.1 (1.1) Ω cm2 vs. healthy: 17.6 (1.7) Ω cm2); fluorescein isothiocyanate (FITC)–dextran (4 kDa) and E. coli Bio-Particle flux. Colonic basal Isc was similar, but duodenal basal Isc was lower in IBS-C (43.5 (4.5) μA cm2) vs. healthy (56.9 (4.9) μA cm2), P=0.05. Ach-evoked ΔIsc was similar.CONCLUSIONS:Females with IBS-C have normal colonic barrier and secretory function. Basal duodenal secretion is decreased in IBS-C.Am J Gastroenterol advance online publication, 21 March 2017; doi:10.1038/ajg.2017.48.

Original languageEnglish (US)
JournalAmerican Journal of Gastroenterology
DOIs
StateAccepted/In press - Mar 21 2017

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Lactulose
Irritable Bowel Syndrome
Tight Junctions
Mannitol
Constipation
Electric Impedance
Endotoxins
Acetylcholine
Claudins
Escherichia coli
Occludin
Publications
Healthy Volunteers
Gene Expression
Messenger RNA
fluorescein isothiocyanate dextran

ASJC Scopus subject areas

  • Gastroenterology

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Constipation-Predominant Irritable Bowel Syndrome Females Have Normal Colonic Barrier and Secretory Function. / Peters, Stephanie A.; Edogawa, Shoko; Sundt, Wendy J.; Dyer, Roy B.; Dalenberg, Daniel A.; Mazzone, Amelia; Singh, Ravinder Jit; Moses, Natalie; Smyrk, Thomas Christopher; Weber, Christopher; Linden, David R; MacNaughton, Wallace K.; Turner, Jerrold R.; Camilleri, Michael; Katzka, David A; Farrugia, Gianrico; Grover, Madhusudan.

In: American Journal of Gastroenterology, 21.03.2017.

Research output: Contribution to journal › Article

Peters, SA, Edogawa, S, Sundt, WJ, Dyer, RB, Dalenberg, DA, Mazzone, A, Singh, RJ, Moses, N, Smyrk, TC, Weber, C, Linden, DR, MacNaughton, WK, Turner, JR, Camilleri, M, Katzka, DA, Farrugia, G & Grover, M 2017, 'Constipation-Predominant Irritable Bowel Syndrome Females Have Normal Colonic Barrier and Secretory Function', American Journal of Gastroenterology. https://doi.org/10.1038/ajg.2017.48
Peters SA, Edogawa S, Sundt WJ, Dyer RB, Dalenberg DA, Mazzone A et al. Constipation-Predominant Irritable Bowel Syndrome Females Have Normal Colonic Barrier and Secretory Function. American Journal of Gastroenterology. 2017 Mar 21. https://doi.org/10.1038/ajg.2017.48
Peters, Stephanie A. ; Edogawa, Shoko ; Sundt, Wendy J. ; Dyer, Roy B. ; Dalenberg, Daniel A. ; Mazzone, Amelia ; Singh, Ravinder Jit ; Moses, Natalie ; Smyrk, Thomas Christopher ; Weber, Christopher ; Linden, David R ; MacNaughton, Wallace K. ; Turner, Jerrold R. ; Camilleri, Michael ; Katzka, David A ; Farrugia, Gianrico ; Grover, Madhusudan. / Constipation-Predominant Irritable Bowel Syndrome Females Have Normal Colonic Barrier and Secretory Function. In: American Journal of Gastroenterology. 2017.
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title = "Constipation-Predominant Irritable Bowel Syndrome Females Have Normal Colonic Barrier and Secretory Function",
abstract = "OBJECTIVES:The objective of this study was to determine whether constipation-predominant irritable bowel syndrome (IBS-C) is associated with changes in intestinal barrier and secretory function.METHODS:A total of 19 IBS-C patients and 18 healthy volunteers (all females) underwent saccharide excretion assay (0.1 g 13C mannitol and 1 g lactulose), measurements of duodenal and colonic mucosal barrier (transmucosal resistance (TMR), macromolecular and Escherichia coli Bio-Particle translocation), mucosal secretion (basal and acetylcholine (Ach)-evoked short-circuit current (Isc)), in vivo duodenal mucosal impedance, circulating endotoxins, and colonic tight junction gene expression.RESULTS:There were no differences in the in vivo measurements of barrier function between IBS-C patients and healthy controls: cumulative excretion of 13C mannitol (0–2 h mean (s.e.m.); IBS-C: 12.1 (0.9) mg vs. healthy: 13.2 (0.8) mg) and lactulose (8–24 h; IBS-C: 0.9 (0.5) mg vs. healthy: 0.5 (0.2) mg); duodenal impedance IBS-C: 729 (65) Ω vs. healthy: 706 (43) Ω; plasma mean endotoxin activity level IBS-C: 0.36 (0.03) vs. healthy: 0.35 (0.02); and in colonic mRNA expression of occludin, zonula occludens (ZO) 1–3, and claudins 1–12 and 14–19. The ex vivo findings were consistent, with no group differences: duodenal TMR (IBS-C: 28.2 (1.9) Ω cm2 vs. healthy: 29.8 (1.9) Ω cm2) and colonic TMR (IBS-C: 19.1 (1.1) Ω cm2 vs. healthy: 17.6 (1.7) Ω cm2); fluorescein isothiocyanate (FITC)–dextran (4 kDa) and E. coli Bio-Particle flux. Colonic basal Isc was similar, but duodenal basal Isc was lower in IBS-C (43.5 (4.5) μA cm2) vs. healthy (56.9 (4.9) μA cm2), P=0.05. Ach-evoked ΔIsc was similar.CONCLUSIONS:Females with IBS-C have normal colonic barrier and secretory function. Basal duodenal secretion is decreased in IBS-C.Am J Gastroenterol advance online publication, 21 March 2017; doi:10.1038/ajg.2017.48.",
author = "Peters, {Stephanie A.} and Shoko Edogawa and Sundt, {Wendy J.} and Dyer, {Roy B.} and Dalenberg, {Daniel A.} and Amelia Mazzone and Singh, {Ravinder Jit} and Natalie Moses and Smyrk, {Thomas Christopher} and Christopher Weber and Linden, {David R} and MacNaughton, {Wallace K.} and Turner, {Jerrold R.} and Michael Camilleri and Katzka, {David A} and Gianrico Farrugia and Madhusudan Grover",
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day = "21",
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TY - JOUR

T1 - Constipation-Predominant Irritable Bowel Syndrome Females Have Normal Colonic Barrier and Secretory Function

AU - Peters, Stephanie A.

AU - Edogawa, Shoko

AU - Sundt, Wendy J.

AU - Dyer, Roy B.

AU - Dalenberg, Daniel A.

AU - Mazzone, Amelia

AU - Singh, Ravinder Jit

AU - Moses, Natalie

AU - Smyrk, Thomas Christopher

AU - Weber, Christopher

AU - Linden, David R

AU - MacNaughton, Wallace K.

AU - Turner, Jerrold R.

AU - Camilleri, Michael

AU - Katzka, David A

AU - Farrugia, Gianrico

AU - Grover, Madhusudan

PY - 2017/3/21

Y1 - 2017/3/21

N2 - OBJECTIVES:The objective of this study was to determine whether constipation-predominant irritable bowel syndrome (IBS-C) is associated with changes in intestinal barrier and secretory function.METHODS:A total of 19 IBS-C patients and 18 healthy volunteers (all females) underwent saccharide excretion assay (0.1 g 13C mannitol and 1 g lactulose), measurements of duodenal and colonic mucosal barrier (transmucosal resistance (TMR), macromolecular and Escherichia coli Bio-Particle translocation), mucosal secretion (basal and acetylcholine (Ach)-evoked short-circuit current (Isc)), in vivo duodenal mucosal impedance, circulating endotoxins, and colonic tight junction gene expression.RESULTS:There were no differences in the in vivo measurements of barrier function between IBS-C patients and healthy controls: cumulative excretion of 13C mannitol (0–2 h mean (s.e.m.); IBS-C: 12.1 (0.9) mg vs. healthy: 13.2 (0.8) mg) and lactulose (8–24 h; IBS-C: 0.9 (0.5) mg vs. healthy: 0.5 (0.2) mg); duodenal impedance IBS-C: 729 (65) Ω vs. healthy: 706 (43) Ω; plasma mean endotoxin activity level IBS-C: 0.36 (0.03) vs. healthy: 0.35 (0.02); and in colonic mRNA expression of occludin, zonula occludens (ZO) 1–3, and claudins 1–12 and 14–19. The ex vivo findings were consistent, with no group differences: duodenal TMR (IBS-C: 28.2 (1.9) Ω cm2 vs. healthy: 29.8 (1.9) Ω cm2) and colonic TMR (IBS-C: 19.1 (1.1) Ω cm2 vs. healthy: 17.6 (1.7) Ω cm2); fluorescein isothiocyanate (FITC)–dextran (4 kDa) and E. coli Bio-Particle flux. Colonic basal Isc was similar, but duodenal basal Isc was lower in IBS-C (43.5 (4.5) μA cm2) vs. healthy (56.9 (4.9) μA cm2), P=0.05. Ach-evoked ΔIsc was similar.CONCLUSIONS:Females with IBS-C have normal colonic barrier and secretory function. Basal duodenal secretion is decreased in IBS-C.Am J Gastroenterol advance online publication, 21 March 2017; doi:10.1038/ajg.2017.48.

AB - OBJECTIVES:The objective of this study was to determine whether constipation-predominant irritable bowel syndrome (IBS-C) is associated with changes in intestinal barrier and secretory function.METHODS:A total of 19 IBS-C patients and 18 healthy volunteers (all females) underwent saccharide excretion assay (0.1 g 13C mannitol and 1 g lactulose), measurements of duodenal and colonic mucosal barrier (transmucosal resistance (TMR), macromolecular and Escherichia coli Bio-Particle translocation), mucosal secretion (basal and acetylcholine (Ach)-evoked short-circuit current (Isc)), in vivo duodenal mucosal impedance, circulating endotoxins, and colonic tight junction gene expression.RESULTS:There were no differences in the in vivo measurements of barrier function between IBS-C patients and healthy controls: cumulative excretion of 13C mannitol (0–2 h mean (s.e.m.); IBS-C: 12.1 (0.9) mg vs. healthy: 13.2 (0.8) mg) and lactulose (8–24 h; IBS-C: 0.9 (0.5) mg vs. healthy: 0.5 (0.2) mg); duodenal impedance IBS-C: 729 (65) Ω vs. healthy: 706 (43) Ω; plasma mean endotoxin activity level IBS-C: 0.36 (0.03) vs. healthy: 0.35 (0.02); and in colonic mRNA expression of occludin, zonula occludens (ZO) 1–3, and claudins 1–12 and 14–19. The ex vivo findings were consistent, with no group differences: duodenal TMR (IBS-C: 28.2 (1.9) Ω cm2 vs. healthy: 29.8 (1.9) Ω cm2) and colonic TMR (IBS-C: 19.1 (1.1) Ω cm2 vs. healthy: 17.6 (1.7) Ω cm2); fluorescein isothiocyanate (FITC)–dextran (4 kDa) and E. coli Bio-Particle flux. Colonic basal Isc was similar, but duodenal basal Isc was lower in IBS-C (43.5 (4.5) μA cm2) vs. healthy (56.9 (4.9) μA cm2), P=0.05. Ach-evoked ΔIsc was similar.CONCLUSIONS:Females with IBS-C have normal colonic barrier and secretory function. Basal duodenal secretion is decreased in IBS-C.Am J Gastroenterol advance online publication, 21 March 2017; doi:10.1038/ajg.2017.48.

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