TY - JOUR
T1 - Considerations for extracellular vesicle and lipoprotein interactions in cell culture assays
AU - Busatto, Sara
AU - Yang, Yubo
AU - Iannotta, Dalila
AU - Davidovich, Irina
AU - Talmon, Yeshayahu
AU - Wolfram, Joy
N1 - Funding Information:
This work is partially supported by the Mayo Clinic Center for Regenerative Medicine in Florida (JW), the Mayo Clinic Center for Biomedical Discovery (JW), the National Institute of Allergy and Infectious Diseases, National Institutes of Health under award numbers R21AI152318 (JW), the American Heart Association under award number 20TPA35490415 (JW), Programma Operativo Nazionale Ricerca e Innovazione, Italian Ministry of Education, University and Research, Italy, under award number DOT13A8025 (DI, JW) and the China Scholarship Council (YY). The content is solely the responsibility of the authors and does not necessarily represent the official views of the funding agencies.
Publisher Copyright:
© 2022 The Authors. Journal of Extracellular Vesicles published by Wiley Periodicals, LLC on behalf of the International Society for Extracellular Vesicles.
PY - 2022/4
Y1 - 2022/4
N2 - With an exponential increase in extracellular vesicle (EV) studies in the past decade, focus has been placed on standardization of experimental design to ensure inter-study comparisons and validity of conclusions. In the case of in vitro assays, the composition of cell culture media is important to consider for EV studies. In particular, levels of lipoproteins, which are critical components of the interstitial fluid, should be taken into consideration. Results from this study reveal that lipoprotein levels in cell culture medium impact the effects that EVs have on recipient cells. Additionally, evidence of EV binding and fusion to lipoprotein-like structures in plasma is provided. However, it is unclear whether the impact of lipoproteins in cell culture is due to direct interactions with EVs, indirect effects, or a combination of both mechanisms. Taken together, cell culture studies performed in the absence of physiological levels of lipoproteins are unlikely to reflect interactions that occur between EVs and recipient cells in an in vivo environment.
AB - With an exponential increase in extracellular vesicle (EV) studies in the past decade, focus has been placed on standardization of experimental design to ensure inter-study comparisons and validity of conclusions. In the case of in vitro assays, the composition of cell culture media is important to consider for EV studies. In particular, levels of lipoproteins, which are critical components of the interstitial fluid, should be taken into consideration. Results from this study reveal that lipoprotein levels in cell culture medium impact the effects that EVs have on recipient cells. Additionally, evidence of EV binding and fusion to lipoprotein-like structures in plasma is provided. However, it is unclear whether the impact of lipoproteins in cell culture is due to direct interactions with EVs, indirect effects, or a combination of both mechanisms. Taken together, cell culture studies performed in the absence of physiological levels of lipoproteins are unlikely to reflect interactions that occur between EVs and recipient cells in an in vivo environment.
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U2 - 10.1002/jev2.12202
DO - 10.1002/jev2.12202
M3 - Letter
C2 - 35362268
AN - SCOPUS:85127426541
SN - 2001-3078
VL - 11
JO - Journal of Extracellular Vesicles
JF - Journal of Extracellular Vesicles
IS - 4
M1 - e12202
ER -