Conformational states of the nuclear pore complex induced by depletion of nuclear Ca2+ stores

Carmen M Terzic, Jason Pyle, Marisa Jaconi, Lisa Stehno-Bittel, David E. Clapham

Research output: Contribution to journalArticle

158 Citations (Scopus)

Abstract

The nuclear pore complex (NPC) is essential for the transit of molecules between the cytoplasm and nucleoplasm of a cell and until recently was thought to allow intermediate-sized molecules (relative molecular mass of ~10,000) to diffuse freely across the nuclear envelope. However, the depletion of calcium from the nuclear envelope of Xenopus laevis oocytes was shown to regulate the passage of intermediate-sized molecules. Two distinct conformational states of the NPC were observed by field emission scanning electron microscopy and atomic force microscopy. A central plug occluded the NPC channel after nuclear calcium stores had been depleted and free diffusion of intermediate-sized molecules had been blocked. Thus, the NPC conformation appears to gate molecular movement across the nuclear envelope.

Original languageEnglish (US)
Pages (from-to)1875-1877
Number of pages3
JournalScience
Volume273
Issue number5283
StatePublished - Sep 27 1996

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Nuclear Pore
Nuclear Envelope
Calcium
Atomic Force Microscopy
Xenopus laevis
Electron Scanning Microscopy
Oocytes
Cytoplasm

ASJC Scopus subject areas

  • General

Cite this

Terzic, C. M., Pyle, J., Jaconi, M., Stehno-Bittel, L., & Clapham, D. E. (1996). Conformational states of the nuclear pore complex induced by depletion of nuclear Ca2+ stores. Science, 273(5283), 1875-1877.

Conformational states of the nuclear pore complex induced by depletion of nuclear Ca2+ stores. / Terzic, Carmen M; Pyle, Jason; Jaconi, Marisa; Stehno-Bittel, Lisa; Clapham, David E.

In: Science, Vol. 273, No. 5283, 27.09.1996, p. 1875-1877.

Research output: Contribution to journalArticle

Terzic, CM, Pyle, J, Jaconi, M, Stehno-Bittel, L & Clapham, DE 1996, 'Conformational states of the nuclear pore complex induced by depletion of nuclear Ca2+ stores', Science, vol. 273, no. 5283, pp. 1875-1877.
Terzic CM, Pyle J, Jaconi M, Stehno-Bittel L, Clapham DE. Conformational states of the nuclear pore complex induced by depletion of nuclear Ca2+ stores. Science. 1996 Sep 27;273(5283):1875-1877.
Terzic, Carmen M ; Pyle, Jason ; Jaconi, Marisa ; Stehno-Bittel, Lisa ; Clapham, David E. / Conformational states of the nuclear pore complex induced by depletion of nuclear Ca2+ stores. In: Science. 1996 ; Vol. 273, No. 5283. pp. 1875-1877.
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