Confocal Microscopy in Ophthalmology

Jay C. Erie, Jay W. McLaren, Sanjay V. Patel

Research output: Contribution to journalArticle

95 Citations (Scopus)

Abstract

Purpose: To describe the principles, capabilities, and applications of confocal microscopy in vivo in ophthalmology. Design: Perspective, literature review, and commentary. Methods: Review and synthesis of selected recent literature, with interpretation and perspective. Results: Confocal microscopy imaging has led to a better understanding of the cellular microstructure in the normal, postsurgical, and diseased cornea by enabling quantitative analysis of the cellular response in the human cornea in vivo. At present, the major role of confocal microscopy is in research of corneal surgery and disease. Clinical applications are limited to facilitating the diagnosis of Acanthamoeba and deep fungal keratitis, measuring residual bed thickness after laser in situ keratomileusis, and measuring endothelial cell density in high-light-scattering situations. Conclusions: In addition to providing qualitative data, confocal microscopy is valuable for quantitative analysis of the cornea and will enable the investigation of pharmacologic and surgical modifications of corneal wound healing, nerve regeneration, and cellular responses. Prospective, quantitative analyses require individual calibration of confocal microscopes for lateral and axial dimensions of images, for image depth, and for light intensity.

Original languageEnglish (US)
Pages (from-to)639-646
Number of pages8
JournalAmerican Journal of Ophthalmology
Volume148
Issue number5
DOIs
StatePublished - Nov 2009

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Ophthalmology
Confocal Microscopy
Cornea
Corneal Diseases
Acanthamoeba
Light
Laser In Situ Keratomileusis
Nerve Regeneration
Keratitis
Wound Healing
Calibration
Endothelial Cells
Cell Count
Research

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Confocal Microscopy in Ophthalmology. / Erie, Jay C.; McLaren, Jay W.; Patel, Sanjay V.

In: American Journal of Ophthalmology, Vol. 148, No. 5, 11.2009, p. 639-646.

Research output: Contribution to journalArticle

Erie, JC, McLaren, JW & Patel, SV 2009, 'Confocal Microscopy in Ophthalmology', American Journal of Ophthalmology, vol. 148, no. 5, pp. 639-646. https://doi.org/10.1016/j.ajo.2009.06.022
Erie, Jay C. ; McLaren, Jay W. ; Patel, Sanjay V. / Confocal Microscopy in Ophthalmology. In: American Journal of Ophthalmology. 2009 ; Vol. 148, No. 5. pp. 639-646.
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