Concurrent treatment with renin-angiotensin system blockers and acetylsalicylic acid reduces nuclear factor κB activation and C-reactive protein expression in human carotid artery plaques

Katherine J E Sattler, Julie E. Woodrum, Offer Galili, Monica Olson, Saquib Samee, Fredric B. Meyer, Xiang Yang Zhu, Lilach O Lerman, Amir Lerman

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Background and Purpose - The local renin-angiotensin system (RAS) and cyclooxygenase-2 contribute to the activation of nuclear factor κB (NFκB) and C-reactive protein (CRP). We hypothesized that the combination of RAS blockers (RASb) and ASA reduces NFκB and CRP within atherosclerotic plaques. Methods - Patients undergoing carotid endarterectomy were divided into groups according to treatment (RASb-acetylsalicylic acid [ASA], ASA, RASb, and control). The expression of NFκB, CRP, and CD40L was analyzed through Western blots in the obtained plaques. Results - Plaques from patients treated with the combination of RASb and ASA showed lower expression of NFκB (25.4±9.8 densitometric units [DU]) than those of the control group (57.6±13.2 DU, P=0.03) as well as lower expression of CRP (20.9±9.6 DU) than those of the other treatment groups (ASA 86.1±13 DU, RASb 88.4±31 DU, controls 67.8±18.6, P=0.004). A negative expression of NFκB was associated with a reduced incidence of symptoms compared with a positive expression (5/33 [15.1%] versus 14/35 [40%], P=0.031). Conclusions - The combined treatment with RASb and ASA decreases the expression of inflammatory markers in atherosclerosis in humans. This study supports the role of the local RAS and cyclooxygenase-2 in the progression of atherosclerosis.

Original languageEnglish (US)
Pages (from-to)14-20
Number of pages7
JournalStroke
Volume36
Issue number1
DOIs
StatePublished - Jan 2005

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Carotid Stenosis
Renin-Angiotensin System
C-Reactive Protein
Aspirin
Cyclooxygenase 2
Atherosclerosis
Therapeutics
CD40 Ligand
Carotid Endarterectomy
Atherosclerotic Plaques
Western Blotting
Control Groups
Incidence

Keywords

  • Aspirin
  • Atherosclerosis
  • Carotid endarterectomy
  • Growth factors
  • Inflammation

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Neuroscience(all)

Cite this

Concurrent treatment with renin-angiotensin system blockers and acetylsalicylic acid reduces nuclear factor κB activation and C-reactive protein expression in human carotid artery plaques. / Sattler, Katherine J E; Woodrum, Julie E.; Galili, Offer; Olson, Monica; Samee, Saquib; Meyer, Fredric B.; Zhu, Xiang Yang; Lerman, Lilach O; Lerman, Amir.

In: Stroke, Vol. 36, No. 1, 01.2005, p. 14-20.

Research output: Contribution to journalArticle

Sattler, Katherine J E ; Woodrum, Julie E. ; Galili, Offer ; Olson, Monica ; Samee, Saquib ; Meyer, Fredric B. ; Zhu, Xiang Yang ; Lerman, Lilach O ; Lerman, Amir. / Concurrent treatment with renin-angiotensin system blockers and acetylsalicylic acid reduces nuclear factor κB activation and C-reactive protein expression in human carotid artery plaques. In: Stroke. 2005 ; Vol. 36, No. 1. pp. 14-20.
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abstract = "Background and Purpose - The local renin-angiotensin system (RAS) and cyclooxygenase-2 contribute to the activation of nuclear factor κB (NFκB) and C-reactive protein (CRP). We hypothesized that the combination of RAS blockers (RASb) and ASA reduces NFκB and CRP within atherosclerotic plaques. Methods - Patients undergoing carotid endarterectomy were divided into groups according to treatment (RASb-acetylsalicylic acid [ASA], ASA, RASb, and control). The expression of NFκB, CRP, and CD40L was analyzed through Western blots in the obtained plaques. Results - Plaques from patients treated with the combination of RASb and ASA showed lower expression of NFκB (25.4±9.8 densitometric units [DU]) than those of the control group (57.6±13.2 DU, P=0.03) as well as lower expression of CRP (20.9±9.6 DU) than those of the other treatment groups (ASA 86.1±13 DU, RASb 88.4±31 DU, controls 67.8±18.6, P=0.004). A negative expression of NFκB was associated with a reduced incidence of symptoms compared with a positive expression (5/33 [15.1{\%}] versus 14/35 [40{\%}], P=0.031). Conclusions - The combined treatment with RASb and ASA decreases the expression of inflammatory markers in atherosclerosis in humans. This study supports the role of the local RAS and cyclooxygenase-2 in the progression of atherosclerosis.",
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AU - Woodrum, Julie E.

AU - Galili, Offer

AU - Olson, Monica

AU - Samee, Saquib

AU - Meyer, Fredric B.

AU - Zhu, Xiang Yang

AU - Lerman, Lilach O

AU - Lerman, Amir

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N2 - Background and Purpose - The local renin-angiotensin system (RAS) and cyclooxygenase-2 contribute to the activation of nuclear factor κB (NFκB) and C-reactive protein (CRP). We hypothesized that the combination of RAS blockers (RASb) and ASA reduces NFκB and CRP within atherosclerotic plaques. Methods - Patients undergoing carotid endarterectomy were divided into groups according to treatment (RASb-acetylsalicylic acid [ASA], ASA, RASb, and control). The expression of NFκB, CRP, and CD40L was analyzed through Western blots in the obtained plaques. Results - Plaques from patients treated with the combination of RASb and ASA showed lower expression of NFκB (25.4±9.8 densitometric units [DU]) than those of the control group (57.6±13.2 DU, P=0.03) as well as lower expression of CRP (20.9±9.6 DU) than those of the other treatment groups (ASA 86.1±13 DU, RASb 88.4±31 DU, controls 67.8±18.6, P=0.004). A negative expression of NFκB was associated with a reduced incidence of symptoms compared with a positive expression (5/33 [15.1%] versus 14/35 [40%], P=0.031). Conclusions - The combined treatment with RASb and ASA decreases the expression of inflammatory markers in atherosclerosis in humans. This study supports the role of the local RAS and cyclooxygenase-2 in the progression of atherosclerosis.

AB - Background and Purpose - The local renin-angiotensin system (RAS) and cyclooxygenase-2 contribute to the activation of nuclear factor κB (NFκB) and C-reactive protein (CRP). We hypothesized that the combination of RAS blockers (RASb) and ASA reduces NFκB and CRP within atherosclerotic plaques. Methods - Patients undergoing carotid endarterectomy were divided into groups according to treatment (RASb-acetylsalicylic acid [ASA], ASA, RASb, and control). The expression of NFκB, CRP, and CD40L was analyzed through Western blots in the obtained plaques. Results - Plaques from patients treated with the combination of RASb and ASA showed lower expression of NFκB (25.4±9.8 densitometric units [DU]) than those of the control group (57.6±13.2 DU, P=0.03) as well as lower expression of CRP (20.9±9.6 DU) than those of the other treatment groups (ASA 86.1±13 DU, RASb 88.4±31 DU, controls 67.8±18.6, P=0.004). A negative expression of NFκB was associated with a reduced incidence of symptoms compared with a positive expression (5/33 [15.1%] versus 14/35 [40%], P=0.031). Conclusions - The combined treatment with RASb and ASA decreases the expression of inflammatory markers in atherosclerosis in humans. This study supports the role of the local RAS and cyclooxygenase-2 in the progression of atherosclerosis.

KW - Aspirin

KW - Atherosclerosis

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KW - Growth factors

KW - Inflammation

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