Concordance of changes in metabolic pathways based on plasma metabolomics and skeletal muscle transcriptomics in type 1 diabetes

Tumpa Dutta, High Seng Chai, Lawrence E. Ward, Aditya Ghosh, Xuan Mai T Persson, G. Charles Ford, Yogish C Kudva, Zhifu D Sun, Yan Asmann, Jean-Pierre Kocher, K Sreekumaran Nair

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Insulin regulates many cellular processes, but the full impact of insulin deficiency on cellular functions remains to be defined. Applying a mass spectrometry-based nontargeted metabolomics approach, we report here alterations of 330 plasma metabolites representing 33 metabolic pathways during an 8-h insulin deprivation in type 1 diabetic individuals. These pathways included those known to be affected by insulin such as glucose, amino acid and lipid metabolism, Krebs cycle, and immune responses and those hitherto unknown to be altered including prostaglandin, arachidonic acid, leukotrienes, neurotransmitters, nucleotides, and anti-inflammatory responses. A significant concordance of metabolome and skeletal muscle transcriptome-based pathways supports an assumption that plasma metabolites are chemical fingerprints of cellular events. Although insulin treatment normalized plasma glucose and many other metabolites, there were 71 metabolites and 24 pathways that differed between non-diabetes and insulin-treated type 1 diabetes. Confirmation of many known pathways altered by insulin using a single blood test offers confidence in the current approach. Future research needs to be focused on newly discovered pathways affected by insulin deficiency and systemic insulin treatment to determine whether they contribute to the high morbidity and mortality in T1D despite insulin treatment.

Original languageEnglish (US)
Pages (from-to)1004-1016
Number of pages13
JournalDiabetes
Volume61
Issue number5
DOIs
StatePublished - May 2012

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Metabolomics
Metabolic Networks and Pathways
Type 1 Diabetes Mellitus
Skeletal Muscle
Insulin
Glucose
Citric Acid Cycle
Metabolome
Leukotrienes
Dermatoglyphics
Hematologic Tests
Lipid Metabolism
Transcriptome
Arachidonic Acid
Prostaglandins
Neurotransmitter Agents
Mass Spectrometry
Anti-Inflammatory Agents
Nucleotides
Morbidity

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism

Cite this

Concordance of changes in metabolic pathways based on plasma metabolomics and skeletal muscle transcriptomics in type 1 diabetes. / Dutta, Tumpa; Chai, High Seng; Ward, Lawrence E.; Ghosh, Aditya; Persson, Xuan Mai T; Ford, G. Charles; Kudva, Yogish C; Sun, Zhifu D; Asmann, Yan; Kocher, Jean-Pierre; Nair, K Sreekumaran.

In: Diabetes, Vol. 61, No. 5, 05.2012, p. 1004-1016.

Research output: Contribution to journalArticle

Dutta, Tumpa ; Chai, High Seng ; Ward, Lawrence E. ; Ghosh, Aditya ; Persson, Xuan Mai T ; Ford, G. Charles ; Kudva, Yogish C ; Sun, Zhifu D ; Asmann, Yan ; Kocher, Jean-Pierre ; Nair, K Sreekumaran. / Concordance of changes in metabolic pathways based on plasma metabolomics and skeletal muscle transcriptomics in type 1 diabetes. In: Diabetes. 2012 ; Vol. 61, No. 5. pp. 1004-1016.
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