Comprehensive proteomic analysis of the human amniotic fluid proteome: Gestational age-dependent changes

John Edward A. Michaels, Surendra Dasari, Leonardo Pereira, Ashok P. Reddy, Jodi A. Lapidus, Xinfang Lu, Thomas Jacob, Archana Thomas, Matthew Rodland, Charles T. Roberts, Michael G. Gravett, Srinivasa R. Nagalla

Research output: Contribution to journalArticle

68 Scopus citations

Abstract

Amniotic fluid (AF) is a significant contributor to fetal health and constitutes a potential rich source of biomarkers for diagnosis of maternal and fetal disorders. In this study, we performed a comprehensive survey of the proteins expressed in AF, combining gel and liquid-based fractionation approaches coupled with LC-MS/MS analysis. Two-dimensional Liquid Chromatography (2D-LC) analysis identified 118 nonredundant proteins with high confidence. One- and two-dimensional gel electrophoresis and in-gel digestion identified 101 proteins. Combining both sets resulted in 219 proteins, of which 96 are unique to AF; 70, 18, and 35 proteins are present in serum, cervico-vaginal fluid, and all three fluids, respectively. Fluorescence two-dimensional differential in-gel electrophoresis (2D-DIGE) comparison of first-, second-, and third-trimester AF samples revealed that maximal differences in the relative abundance of AF proteins occur between the first and second trimesters. A systematic analysis of proteins present both in AF and maternal serum could lead to the development of new noninvasive diagnostic procedures to monitor fetal status.

Original languageEnglish (US)
Pages (from-to)1277-1285
Number of pages9
JournalJournal of Proteome Research
Volume6
Issue number4
DOIs
StatePublished - Apr 1 2007

Keywords

  • Amniotic fluid
  • Aneuploidy
  • Biomarkers
  • Pregnancy
  • Proteome

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

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  • Cite this

    Michaels, J. E. A., Dasari, S., Pereira, L., Reddy, A. P., Lapidus, J. A., Lu, X., Jacob, T., Thomas, A., Rodland, M., Roberts, C. T., Gravett, M. G., & Nagalla, S. R. (2007). Comprehensive proteomic analysis of the human amniotic fluid proteome: Gestational age-dependent changes. Journal of Proteome Research, 6(4), 1277-1285. https://doi.org/10.1021/pr060543t