Comprehensive proteomic analysis of human pancreatic juice

Mads Grønborg, Jakob Bunkenborg, Troels Zakarias Kristiansen, Ole Nørregaard Jensen, Charles J. Yeo, Ralph H. Hruban, Anirban Maitra, Michael G. Goggins, Akhilesh Pandey

Research output: Contribution to journalArticle

156 Scopus citations

Abstract

Proteomic technologies provide an excellent means for analysis of body fluids for cataloging protein constituents and identifying biomarkers for early detection of cancers. The biomarkers currently available for pancreatic cancer, such as CA19-9, lack adequate sensitivity and specificity contributing to late diagnosis of this deadly disease. In this study, we carried out a comprehensive characterization of the "pancreatic juice proteome" in patients with pancreatic adenocarcinoma. Pancreatic juice was first fractionated by 1-dimensional gel electrophoresis and subsequently analyzed by liquid chromatography tandem mass spectrometry (LC-MS/MS). A total of 170 unique proteins were identified including known pancreatic cancer tumor markers (e.g., CEA, MUC1) and proteins overexpressed in pancreatic cancers (e.g., hepatocarcinoma-intestine-pancreas/pancreatitis-associated protein (HIP/PAP) and lipocalin 2). In addition, we identified a number of proteins that have not been previously described in pancreatic juice (e.g., tumor rejection antigen (pg96) and azurocidin). Interestingly, a novel protein that is 85% identical to HIP/PAP was identified, which we have designated as PAP-2. The proteins identified in this study could be directly assessed for their potential as biomarkers for pancreatic cancer by quantitative proteomics methods or immunoassays.

Original languageEnglish (US)
Pages (from-to)1042-1055
Number of pages14
JournalJournal of Proteome Research
Volume3
Issue number5
DOIs
StatePublished - Sep 1 2004
Externally publishedYes

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Keywords

  • Body fluid
  • Functional annotation
  • Liquid chromatography tandem mass spectrometry (LC-MS/MS)

ASJC Scopus subject areas

  • Genetics
  • Biotechnology
  • Biochemistry

Cite this

Grønborg, M., Bunkenborg, J., Kristiansen, T. Z., Jensen, O. N., Yeo, C. J., Hruban, R. H., Maitra, A., Goggins, M. G., & Pandey, A. (2004). Comprehensive proteomic analysis of human pancreatic juice. Journal of Proteome Research, 3(5), 1042-1055. https://doi.org/10.1021/pr0499085