Comprehensive proteomic analysis of human cervical-vaginal fluid

Surendra Dasari, Leonardo Pereira, Ashok P. Reddy, John Edward A. Michaels, Xinfang Lu, Thomas Jacob, Archana Thomas, Matthew Rodland, Charles T. Roberts, Michael G. Gravett, Srinivasa R. Nagalla

Research output: Contribution to journalArticle

98 Scopus citations

Abstract

Cervical-vaginal fluid (CVF) is a potential rich source of biomarkers for enhancing our understanding of human parturition and pathologic conditions affecting pregnancy. In this study, we performed a comprehensive survey of the CVF proteome in pregnancy utilizing multidimensional liquid chromatography (2D-LC) coupled with mass spectrometry and gel-electrophoresis-based protein separation and identification. In total, 150 unique proteins were identified using multiple protein identification algorithms. Metabolism (32%) and immune response-related (22%) proteins are the major functional categories represented in the CVF proteome. A comparison of the CVF, serum, and amniotic fluid proteomes showed that 77 proteins are unique to CVF, while 56 and 17 CVF proteins also occur in serum and amniotic fluid, respectively. This data set provides a foundation for evaluation of these proteins as potential CVF biomarkers for noninvasive diagnosis of pregnancy-related disorders.

Original languageEnglish (US)
Pages (from-to)1258-1268
Number of pages11
JournalJournal of Proteome Research
Volume6
Issue number4
DOIs
StatePublished - Apr 1 2007

Keywords

  • Pregnancy
  • Prematurity
  • Proteome
  • Vaginal fluid

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

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