Comprehensive detection of chromosomal translocations in lymphoproliferative disorders by massively parallel sequencing

Philippe Szankasi, Ashini Bolia, Michael Liew, Jonathan A. Schumacher, Elaine P.S. Gee, Anna P. Matynia, K. David Li, Jay L. Patel, Xinjie Xu, Mohamed E. Salama, Todd W. Kelley

Research output: Contribution to journalArticle

Abstract

Balanced translocations have diagnostic and prognostic value in B-cell lymphoproliferative disorders (LPDs). Most of these translocations involve the juxtaposition of a strong immunoglobulin (Ig) enhancer to proto-oncogenes, such as BCL2, BCL6, and MYC, leading to their overexpression. These rearrangements generally do not result in mRNA fusions, and fluorescent in situ hybridization (FISH) remains the gold standard for assessing of recurrent translocations in LPDs. With the growing use of massively parallel sequencing for the detection of both point mutations and large structural rearrangements, we aimed at evaluating the utility of this method for the molecular work-up of B-cell LPDs side by side with FISH. We describe a method using solution capture for enrichment of known translocation breakpoints and massively parallel sequencing for the detection of balanced translocation in formalin-fixed tissues with a B-cell LPD. We detected a total of 57 rearrangements with a high concordance of 94.2% when compared to FISH. We detected translocations between BCL2, BCL6, and MYC and the three Ig loci and non-Ig loci, including novel partners for MYC and BCL6. In addition, massively parallel sequencing allowed a detailed analysis of the structure of the resulting chromosomal fusions. Our comparison shows the feasibility of using massively parallel sequencing for detecting balanced translocations in B-cell LPDs and advantages and disadvantages to both methods, and how they can complement each other.

Original languageEnglish (US)
Pages (from-to)121-133
Number of pages13
JournalJournal of Hematopathology
Volume12
Issue number3
DOIs
StatePublished - Sep 1 2019

Keywords

  • B-cell lymphoma
  • Chromosomal translocation
  • Massively parallel sequencing
  • VDJ recombination

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology
  • Hematology

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  • Cite this

    Szankasi, P., Bolia, A., Liew, M., Schumacher, J. A., Gee, E. P. S., Matynia, A. P., Li, K. D., Patel, J. L., Xu, X., Salama, M. E., & Kelley, T. W. (2019). Comprehensive detection of chromosomal translocations in lymphoproliferative disorders by massively parallel sequencing. Journal of Hematopathology, 12(3), 121-133. https://doi.org/10.1007/s12308-019-00360-0