TY - JOUR
T1 - Comprehensive and innovative techniques for liver transplantation in rats
T2 - A surgical guide
AU - Hori, Tomohide
AU - Nguyen, Justin H.
AU - Zhao, Xiangdong
AU - Ogura, Yasuhiro
AU - Hata, Toshiyuki
AU - Yagi, Shintaro
AU - Chen, Feng
AU - Baine, Ann Marie T.
AU - Ohashi, Norifumi
AU - Eckman, Christopher B.
AU - Herdt, Aimee R.
AU - Egawa, Hiroto
AU - Takada, Yasutsugu
AU - Oike, Fumitaka
AU - Sakamoto, Seisuke
AU - Kasahara, Mureo
AU - Ogawa, Kohei
AU - Hata, Koichiro
AU - Iida, Taku
AU - Yonekawa, Yukihide
AU - Sibulesky, Lena
AU - Kuribayashi, Kagemasa
AU - Kato, Takuma
AU - Saito, Kanako
AU - Wang, Linan
AU - Torii, Mie
AU - Sahara, Naruhiko
AU - Kamo, Naoko
AU - Sahara, Tomoko
AU - Yasutomi, Motohiko
AU - Uemoto, Shinji
PY - 2010/7/7
Y1 - 2010/7/7
N2 - AIM: To investigate our learning curves of orthotopic liver transplantation (OLT) in rats and the most important factor for successful surgery. METHODS: We describe the surgical procedures for our rat OLT model, and determined the operator learning curves. The various factors that contributed to successful surgery were determined. The most important surgical factors were evaluated between successful and unsuccessful surgeries. RESULTS: Learning curve data indicated that 50 cases were required for operator training to start a study. Operative time, blood loss, warm ischemic time, anhepatic phase, unstable systemic hemodynamic state, and body temperature after surgery significantly affected surgery success by univariate analysis, while the anhepatic phase was the most critical factor for success by multivariate analysis. CONCLUSION: OLT in rats is the only liver transplantation model that provides clinically relevant and reliable results. Shortened anhepatic phase is key to success in this model.
AB - AIM: To investigate our learning curves of orthotopic liver transplantation (OLT) in rats and the most important factor for successful surgery. METHODS: We describe the surgical procedures for our rat OLT model, and determined the operator learning curves. The various factors that contributed to successful surgery were determined. The most important surgical factors were evaluated between successful and unsuccessful surgeries. RESULTS: Learning curve data indicated that 50 cases were required for operator training to start a study. Operative time, blood loss, warm ischemic time, anhepatic phase, unstable systemic hemodynamic state, and body temperature after surgery significantly affected surgery success by univariate analysis, while the anhepatic phase was the most critical factor for success by multivariate analysis. CONCLUSION: OLT in rats is the only liver transplantation model that provides clinically relevant and reliable results. Shortened anhepatic phase is key to success in this model.
KW - Animal model
KW - Liver transplantation
KW - Microsurgery
KW - Rat
KW - Reperfusion injury
KW - Ultra-microsurgery
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U2 - 10.3748/wjg.v16.i25.3120
DO - 10.3748/wjg.v16.i25.3120
M3 - Article
C2 - 20593497
AN - SCOPUS:77954376351
SN - 1007-9327
VL - 16
SP - 3120
EP - 3132
JO - World Journal of Gastroenterology
JF - World Journal of Gastroenterology
IS - 25
ER -