Comprehensive analysis of tumor microenvironment cytokines in Waldenstrom macroglobulinemia identifies CCL5 as a novel modulator of IL-6 activity

Sherine F. Elsawa, Anne J Novak, Steven C. Ziesmer, Luciana L. Almada, Lucy S. Hodge, Deanna M. Grote, Thomas Elmer Witzig, Martin E Fernandez-Zapico, Stephen Maxted Ansell

Research output: Contribution to journalArticle

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Abstract

Although proinflammatory and chemotactic cytokines can profoundly affect the tumor microenvironment, and many of them have been shown to have therapeutic efficacy in preclinical models, the role of these molecules in Waldenström macroglobulinemia (WM) remains poorly understood. In this study, simultaneous analysis of WM patient sera and bone marrow biopsies identified a set of dysregulated cytokines including CCL5, G-CSF, and soluble IL-2 receptor, that were significantly elevated in WM patients whereas IL-8 and EGF levels were significantly lower in these patients compared with healthy controls. Interestingly, CCL5 levels positively correlated with features of disease aggressiveness such as elevated IgM levels and bone marrow involvement. Functional analysis of tumor microenvironment revealed a functional correlation between CCL5 levels and IL-6 levels, a proinflammatory cytokine with an important role in normal and malignant B-cell biology. Furthermore, CCL5 stimulated IL-6 secretion in WM stromal cells resulting in increased IgM secretion by WM malignant cells via the JAK/STAT signaling pathway. Thus, together these results define a novel signaling network in the WM tumor microenvironment controlling IgM secretion and suggest CCL5 as a potential target for the treatment of this disease.

Original languageEnglish (US)
Pages (from-to)5540-5549
Number of pages10
JournalBlood
Volume118
Issue number20
DOIs
StatePublished - Nov 17 2011

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Waldenstrom Macroglobulinemia
Tumor Microenvironment
Modulators
Immunoglobulin M
Tumors
Interleukin-6
Cytokines
Bone
Cytology
Functional analysis
Biopsy
Interleukin-2 Receptors
Granulocyte Colony-Stimulating Factor
Interleukin-8
Chemokines
Epidermal Growth Factor
Bone Marrow
Molecules
Stromal Cells
Cell Biology

ASJC Scopus subject areas

  • Hematology
  • Biochemistry
  • Cell Biology
  • Immunology

Cite this

Comprehensive analysis of tumor microenvironment cytokines in Waldenstrom macroglobulinemia identifies CCL5 as a novel modulator of IL-6 activity. / Elsawa, Sherine F.; Novak, Anne J; Ziesmer, Steven C.; Almada, Luciana L.; Hodge, Lucy S.; Grote, Deanna M.; Witzig, Thomas Elmer; Fernandez-Zapico, Martin E; Ansell, Stephen Maxted.

In: Blood, Vol. 118, No. 20, 17.11.2011, p. 5540-5549.

Research output: Contribution to journalArticle

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abstract = "Although proinflammatory and chemotactic cytokines can profoundly affect the tumor microenvironment, and many of them have been shown to have therapeutic efficacy in preclinical models, the role of these molecules in Waldenstr{\"o}m macroglobulinemia (WM) remains poorly understood. In this study, simultaneous analysis of WM patient sera and bone marrow biopsies identified a set of dysregulated cytokines including CCL5, G-CSF, and soluble IL-2 receptor, that were significantly elevated in WM patients whereas IL-8 and EGF levels were significantly lower in these patients compared with healthy controls. Interestingly, CCL5 levels positively correlated with features of disease aggressiveness such as elevated IgM levels and bone marrow involvement. Functional analysis of tumor microenvironment revealed a functional correlation between CCL5 levels and IL-6 levels, a proinflammatory cytokine with an important role in normal and malignant B-cell biology. Furthermore, CCL5 stimulated IL-6 secretion in WM stromal cells resulting in increased IgM secretion by WM malignant cells via the JAK/STAT signaling pathway. Thus, together these results define a novel signaling network in the WM tumor microenvironment controlling IgM secretion and suggest CCL5 as a potential target for the treatment of this disease.",
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AU - Fernandez-Zapico, Martin E

AU - Ansell, Stephen Maxted

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