Compound-specific carbon isotope ratio determination of enriched cholesterol

Z. K. Guo, A. H. Luke, W. P. Lee, D. Schoeller

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Abstract

On-line gas chromatography/combustion/isotope ratio mass spectrometry (GC/C/IRMS) was evaluated for use in stable isotope studies of human cholesterol metabolism. Calibration curves were constructed by analyzing underivatized and TMS-cholesterol over a range of 13C abundances from natural abundance to 0.05 mol of [3,4-13C]cholesterol per mole of unlabeled cholesterol (δ 13C -24 to 270‰). The calibration curves were highly linear (r2 = 0.99) with slopes of 0.98 and 0.88 for underivatized and TMS-cholesterol, respectively. Higher precision of 13C/12C ratio determination was obtained using TMS-cholesterol, with a detection limit (2 SD) of 0.3‰ or 0.004 mol% of [3,4-13C]-cholesterol. By comparison standard GC/MS had a detection limit of 0.06 mol% for [3,4-13C]cholesterol. A 57-day study of cholesterol elimination was conducted in one human subject with oral administration of 50 mg of [3,4-13C]cholesterol. Peak enrichment was 9‰ above baseline (40 times the detection limit) and could be followed for 40-50 days. The results indicate that GC/C/IRMS provides 15-fold lower detection limits for [3,4-13C]-choleseterol than GC/MS and is useful for human studies of cholesterol metabolism.

Original languageEnglish (US)
Pages (from-to)1954-1959
Number of pages6
JournalAnalytical Chemistry
Volume65
Issue number15
StatePublished - 1993
Externally publishedYes

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Carbon Isotopes
Cholesterol
Isotopes
Metabolism
Gas chromatography
Mass spectrometry
Calibration

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Guo, Z. K., Luke, A. H., Lee, W. P., & Schoeller, D. (1993). Compound-specific carbon isotope ratio determination of enriched cholesterol. Analytical Chemistry, 65(15), 1954-1959.

Compound-specific carbon isotope ratio determination of enriched cholesterol. / Guo, Z. K.; Luke, A. H.; Lee, W. P.; Schoeller, D.

In: Analytical Chemistry, Vol. 65, No. 15, 1993, p. 1954-1959.

Research output: Contribution to journalArticle

Guo, ZK, Luke, AH, Lee, WP & Schoeller, D 1993, 'Compound-specific carbon isotope ratio determination of enriched cholesterol', Analytical Chemistry, vol. 65, no. 15, pp. 1954-1959.
Guo ZK, Luke AH, Lee WP, Schoeller D. Compound-specific carbon isotope ratio determination of enriched cholesterol. Analytical Chemistry. 1993;65(15):1954-1959.
Guo, Z. K. ; Luke, A. H. ; Lee, W. P. ; Schoeller, D. / Compound-specific carbon isotope ratio determination of enriched cholesterol. In: Analytical Chemistry. 1993 ; Vol. 65, No. 15. pp. 1954-1959.
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