Composition of protein supplements used for human embryo culture

Dean E. Morbeck, Melissa Paczkowski, Jolene R. Fredrickson, Rebecca L. Krisher, Heather S. Hoff, Nikola A. Baumann, Thomas Moyer, Dietrich Matern

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Purpose: To determine the composition of commercially available protein supplements for embryo culture media and test if differences in protein supplement composition are biologically relevant in a murine model.

Methods: Amino acid, organic acid, ion and metal content were determined for 6 protein supplements: recombinant human albumin (AlbIX), human serum albumin (HSA and Buminate), and three complex protein supplements (SSS, SPS, LGPS). To determine if differences in the composition of these supplements are biologically relevant, mouse one-cell embryos were collected and cultured for 120 hours in each protein supplement in Global media at 5 and 20 % oxygen in an EmbryoScope time-lapse incubator. The compositions of six protein supplements were analyzed for concentrations of 39 individual amino acids, organic acids, ions and elements. Blastocyst development and cell cycle timings were calculated at 96-hours of culture and the experiments were repeated in triplicate. Blastocyst gene expression was analyzed.

Results: Recombinant albumin had the fewest undefined components , the lowest concentration of elements detected, and resulted in high blastocyst development in both 5 and 20 % oxygen. Buminate, LGPS and SPS had high levels of transition metals whereas SSS had high concentrations of amino acids. Pre-compaction mouse embryo development was delayed relative to embryos in AlbIX for all supplements and blastocyst formation was reduced in Buminate, SPS and SSS.

Conclusions: The composition of protein supplements are variable, consisting of previously undescribed components. High concentrations of pro-oxidant transition metals were most notable. Blastocyst development was protein dependent and showed an interaction with oxygen concentration and pro-oxidant supplements.

Original languageEnglish (US)
Pages (from-to)1703-1711
Number of pages9
JournalJournal of Assisted Reproduction and Genetics
Volume31
Issue number12
DOIs
StatePublished - Dec 2 2014

Fingerprint

Embryonic Structures
Blastocyst
Proteins
Metals
Oxygen
Amino Acids
Fetoscopes
Albumins
Reactive Oxygen Species
Ions
Incubators
Acids
Recombinant Proteins
Serum Albumin
Embryonic Development
Culture Media
Cell Cycle
Gene Expression

Keywords

  • Albumin
  • Embryo culture
  • Mouse embryo assay
  • Protein
  • Quality control

ASJC Scopus subject areas

  • Obstetrics and Gynecology
  • Reproductive Medicine
  • Developmental Biology
  • Genetics
  • Genetics(clinical)

Cite this

Morbeck, D. E., Paczkowski, M., Fredrickson, J. R., Krisher, R. L., Hoff, H. S., Baumann, N. A., ... Matern, D. (2014). Composition of protein supplements used for human embryo culture. Journal of Assisted Reproduction and Genetics, 31(12), 1703-1711. https://doi.org/10.1007/s10815-014-0349-2

Composition of protein supplements used for human embryo culture. / Morbeck, Dean E.; Paczkowski, Melissa; Fredrickson, Jolene R.; Krisher, Rebecca L.; Hoff, Heather S.; Baumann, Nikola A.; Moyer, Thomas; Matern, Dietrich.

In: Journal of Assisted Reproduction and Genetics, Vol. 31, No. 12, 02.12.2014, p. 1703-1711.

Research output: Contribution to journalArticle

Morbeck, DE, Paczkowski, M, Fredrickson, JR, Krisher, RL, Hoff, HS, Baumann, NA, Moyer, T & Matern, D 2014, 'Composition of protein supplements used for human embryo culture', Journal of Assisted Reproduction and Genetics, vol. 31, no. 12, pp. 1703-1711. https://doi.org/10.1007/s10815-014-0349-2
Morbeck DE, Paczkowski M, Fredrickson JR, Krisher RL, Hoff HS, Baumann NA et al. Composition of protein supplements used for human embryo culture. Journal of Assisted Reproduction and Genetics. 2014 Dec 2;31(12):1703-1711. https://doi.org/10.1007/s10815-014-0349-2
Morbeck, Dean E. ; Paczkowski, Melissa ; Fredrickson, Jolene R. ; Krisher, Rebecca L. ; Hoff, Heather S. ; Baumann, Nikola A. ; Moyer, Thomas ; Matern, Dietrich. / Composition of protein supplements used for human embryo culture. In: Journal of Assisted Reproduction and Genetics. 2014 ; Vol. 31, No. 12. pp. 1703-1711.
@article{5bb98b5728bb491a9347f0a62e952ccd,
title = "Composition of protein supplements used for human embryo culture",
abstract = "Purpose: To determine the composition of commercially available protein supplements for embryo culture media and test if differences in protein supplement composition are biologically relevant in a murine model.Methods: Amino acid, organic acid, ion and metal content were determined for 6 protein supplements: recombinant human albumin (AlbIX), human serum albumin (HSA and Buminate), and three complex protein supplements (SSS, SPS, LGPS). To determine if differences in the composition of these supplements are biologically relevant, mouse one-cell embryos were collected and cultured for 120 hours in each protein supplement in Global media at 5 and 20 {\%} oxygen in an EmbryoScope time-lapse incubator. The compositions of six protein supplements were analyzed for concentrations of 39 individual amino acids, organic acids, ions and elements. Blastocyst development and cell cycle timings were calculated at 96-hours of culture and the experiments were repeated in triplicate. Blastocyst gene expression was analyzed.Results: Recombinant albumin had the fewest undefined components , the lowest concentration of elements detected, and resulted in high blastocyst development in both 5 and 20 {\%} oxygen. Buminate, LGPS and SPS had high levels of transition metals whereas SSS had high concentrations of amino acids. Pre-compaction mouse embryo development was delayed relative to embryos in AlbIX for all supplements and blastocyst formation was reduced in Buminate, SPS and SSS.Conclusions: The composition of protein supplements are variable, consisting of previously undescribed components. High concentrations of pro-oxidant transition metals were most notable. Blastocyst development was protein dependent and showed an interaction with oxygen concentration and pro-oxidant supplements.",
keywords = "Albumin, Embryo culture, Mouse embryo assay, Protein, Quality control",
author = "Morbeck, {Dean E.} and Melissa Paczkowski and Fredrickson, {Jolene R.} and Krisher, {Rebecca L.} and Hoff, {Heather S.} and Baumann, {Nikola A.} and Thomas Moyer and Dietrich Matern",
year = "2014",
month = "12",
day = "2",
doi = "10.1007/s10815-014-0349-2",
language = "English (US)",
volume = "31",
pages = "1703--1711",
journal = "Journal of Assisted Reproduction and Genetics",
issn = "1058-0468",
publisher = "Springer New York",
number = "12",

}

TY - JOUR

T1 - Composition of protein supplements used for human embryo culture

AU - Morbeck, Dean E.

AU - Paczkowski, Melissa

AU - Fredrickson, Jolene R.

AU - Krisher, Rebecca L.

AU - Hoff, Heather S.

AU - Baumann, Nikola A.

AU - Moyer, Thomas

AU - Matern, Dietrich

PY - 2014/12/2

Y1 - 2014/12/2

N2 - Purpose: To determine the composition of commercially available protein supplements for embryo culture media and test if differences in protein supplement composition are biologically relevant in a murine model.Methods: Amino acid, organic acid, ion and metal content were determined for 6 protein supplements: recombinant human albumin (AlbIX), human serum albumin (HSA and Buminate), and three complex protein supplements (SSS, SPS, LGPS). To determine if differences in the composition of these supplements are biologically relevant, mouse one-cell embryos were collected and cultured for 120 hours in each protein supplement in Global media at 5 and 20 % oxygen in an EmbryoScope time-lapse incubator. The compositions of six protein supplements were analyzed for concentrations of 39 individual amino acids, organic acids, ions and elements. Blastocyst development and cell cycle timings were calculated at 96-hours of culture and the experiments were repeated in triplicate. Blastocyst gene expression was analyzed.Results: Recombinant albumin had the fewest undefined components , the lowest concentration of elements detected, and resulted in high blastocyst development in both 5 and 20 % oxygen. Buminate, LGPS and SPS had high levels of transition metals whereas SSS had high concentrations of amino acids. Pre-compaction mouse embryo development was delayed relative to embryos in AlbIX for all supplements and blastocyst formation was reduced in Buminate, SPS and SSS.Conclusions: The composition of protein supplements are variable, consisting of previously undescribed components. High concentrations of pro-oxidant transition metals were most notable. Blastocyst development was protein dependent and showed an interaction with oxygen concentration and pro-oxidant supplements.

AB - Purpose: To determine the composition of commercially available protein supplements for embryo culture media and test if differences in protein supplement composition are biologically relevant in a murine model.Methods: Amino acid, organic acid, ion and metal content were determined for 6 protein supplements: recombinant human albumin (AlbIX), human serum albumin (HSA and Buminate), and three complex protein supplements (SSS, SPS, LGPS). To determine if differences in the composition of these supplements are biologically relevant, mouse one-cell embryos were collected and cultured for 120 hours in each protein supplement in Global media at 5 and 20 % oxygen in an EmbryoScope time-lapse incubator. The compositions of six protein supplements were analyzed for concentrations of 39 individual amino acids, organic acids, ions and elements. Blastocyst development and cell cycle timings were calculated at 96-hours of culture and the experiments were repeated in triplicate. Blastocyst gene expression was analyzed.Results: Recombinant albumin had the fewest undefined components , the lowest concentration of elements detected, and resulted in high blastocyst development in both 5 and 20 % oxygen. Buminate, LGPS and SPS had high levels of transition metals whereas SSS had high concentrations of amino acids. Pre-compaction mouse embryo development was delayed relative to embryos in AlbIX for all supplements and blastocyst formation was reduced in Buminate, SPS and SSS.Conclusions: The composition of protein supplements are variable, consisting of previously undescribed components. High concentrations of pro-oxidant transition metals were most notable. Blastocyst development was protein dependent and showed an interaction with oxygen concentration and pro-oxidant supplements.

KW - Albumin

KW - Embryo culture

KW - Mouse embryo assay

KW - Protein

KW - Quality control

UR - http://www.scopus.com/inward/record.url?scp=84914692162&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84914692162&partnerID=8YFLogxK

U2 - 10.1007/s10815-014-0349-2

DO - 10.1007/s10815-014-0349-2

M3 - Article

VL - 31

SP - 1703

EP - 1711

JO - Journal of Assisted Reproduction and Genetics

JF - Journal of Assisted Reproduction and Genetics

SN - 1058-0468

IS - 12

ER -