Composition of potassium channels in normal rat coronary smooth muscle cells and activation effects of docosahexaenoic acid

L. L. Qian, R. X. Wang, M. Q. Sun, D. Y. Xia, X. Tang, Y. Ji, Y. Wu, X. Y. Liu, S. P. Dang, Q. Chai, Tong D Lu

Research output: Contribution to journalArticle

Abstract

RESULTS: Potassium currents were significantly increased with 5 μmol/L DHA perfusion (P<0.05). The current density was increased from (52.80±6.68) pA/pF to (110.09±13.39) pA/pF (P<0.05) after DHA perfusion when the stimulation voltage was 100 mV.Compared with baseline, potassium currents were significantly decreased by various inhibitor perfusion (tetraethylammonium: (49.63±5.75) pA/pF vs. (13.96±2.18) pA/pF; ibritoxin: (50.67±7.89) pA/pF vs. (26.53±4.68) pA/pF; TRAM-34: (52.60±7.02) pA/pF vs. (46.05±7.60) pA/pF; apamin: (51.97±3.83) pA/pF vs. (44.89±5.04) pA/pF; 4-aminopyridine: (51.19±3.44) pA/pF vs. (29.92±2.81) pA/pF; glyburide: (49.67±1.77) pA/pF vs. (49.61±1.87) pA/pF, all P<0.05). In presence of different inhibitors, potassium channel current densities were increased after DHA perfusion except tetraethylammonium (tetraethylammonium: ( 12.79±1.89) pA/pF; ibritoxin: (67.08±5.54) pA/pF; TRAM-34: (117.91±21.79) pA/pF; apamin: (108.33±7.06) pA/pF; 4-aminopyridine: (127.73±20.56) pA/pF; glyburide: (121.53±13.83) pA/pF, all P<0.05 compared with baseline).

CONCLUSIONS: Large-conductance calcium-activated potassium channel and voltage-gated potassium channel are the major constituents of potassium channels in CASMCs.DHA can activate potassium channels in CASMCs, mainly the large conductance calcium-activated potassium channel, thus dilate coronary arteries.

OBJECTIVE: To investigate the composition of potassium channels in normal rat coronary smooth muscle cells (CASMCs) and the activation effects of docosahexaenoic acid (DHA).

METHODS: CASMCs were isolated by enzyme digestion.Effects of different types of potassium channel blockers and/or DHA on potassium channels currents were studied by whole-cell patch clamp technique.

Original languageEnglish (US)
Pages (from-to)600-604
Number of pages5
JournalZhonghua xin xue guan bing za zhi
Volume44
Issue number7
DOIs
StatePublished - Jul 24 2016

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Docosahexaenoic Acids
Potassium Channels
Smooth Muscle Myocytes
Tetraethylammonium
Perfusion
Large-Conductance Calcium-Activated Potassium Channels
Apamin
4-Aminopyridine
Glyburide
Potassium
Potassium Channel Blockers
Voltage-Gated Potassium Channels
Patch-Clamp Techniques
Digestion
Coronary Vessels
Enzymes

ASJC Scopus subject areas

  • Medicine(all)
  • Cardiology and Cardiovascular Medicine

Cite this

Composition of potassium channels in normal rat coronary smooth muscle cells and activation effects of docosahexaenoic acid. / Qian, L. L.; Wang, R. X.; Sun, M. Q.; Xia, D. Y.; Tang, X.; Ji, Y.; Wu, Y.; Liu, X. Y.; Dang, S. P.; Chai, Q.; Lu, Tong D.

In: Zhonghua xin xue guan bing za zhi, Vol. 44, No. 7, 24.07.2016, p. 600-604.

Research output: Contribution to journalArticle

Qian, L. L. ; Wang, R. X. ; Sun, M. Q. ; Xia, D. Y. ; Tang, X. ; Ji, Y. ; Wu, Y. ; Liu, X. Y. ; Dang, S. P. ; Chai, Q. ; Lu, Tong D. / Composition of potassium channels in normal rat coronary smooth muscle cells and activation effects of docosahexaenoic acid. In: Zhonghua xin xue guan bing za zhi. 2016 ; Vol. 44, No. 7. pp. 600-604.
@article{c5e77542d8ff45faae54de928538e0ac,
title = "Composition of potassium channels in normal rat coronary smooth muscle cells and activation effects of docosahexaenoic acid",
abstract = "RESULTS: Potassium currents were significantly increased with 5 μmol/L DHA perfusion (P<0.05). The current density was increased from (52.80±6.68) pA/pF to (110.09±13.39) pA/pF (P<0.05) after DHA perfusion when the stimulation voltage was 100 mV.Compared with baseline, potassium currents were significantly decreased by various inhibitor perfusion (tetraethylammonium: (49.63±5.75) pA/pF vs. (13.96±2.18) pA/pF; ibritoxin: (50.67±7.89) pA/pF vs. (26.53±4.68) pA/pF; TRAM-34: (52.60±7.02) pA/pF vs. (46.05±7.60) pA/pF; apamin: (51.97±3.83) pA/pF vs. (44.89±5.04) pA/pF; 4-aminopyridine: (51.19±3.44) pA/pF vs. (29.92±2.81) pA/pF; glyburide: (49.67±1.77) pA/pF vs. (49.61±1.87) pA/pF, all P<0.05). In presence of different inhibitors, potassium channel current densities were increased after DHA perfusion except tetraethylammonium (tetraethylammonium: ( 12.79±1.89) pA/pF; ibritoxin: (67.08±5.54) pA/pF; TRAM-34: (117.91±21.79) pA/pF; apamin: (108.33±7.06) pA/pF; 4-aminopyridine: (127.73±20.56) pA/pF; glyburide: (121.53±13.83) pA/pF, all P<0.05 compared with baseline).CONCLUSIONS: Large-conductance calcium-activated potassium channel and voltage-gated potassium channel are the major constituents of potassium channels in CASMCs.DHA can activate potassium channels in CASMCs, mainly the large conductance calcium-activated potassium channel, thus dilate coronary arteries.OBJECTIVE: To investigate the composition of potassium channels in normal rat coronary smooth muscle cells (CASMCs) and the activation effects of docosahexaenoic acid (DHA).METHODS: CASMCs were isolated by enzyme digestion.Effects of different types of potassium channel blockers and/or DHA on potassium channels currents were studied by whole-cell patch clamp technique.",
author = "Qian, {L. L.} and Wang, {R. X.} and Sun, {M. Q.} and Xia, {D. Y.} and X. Tang and Y. Ji and Y. Wu and Liu, {X. Y.} and Dang, {S. P.} and Q. Chai and Lu, {Tong D}",
year = "2016",
month = "7",
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doi = "10.3760/cma.j.issn.0253-3758.2016.07.009",
language = "English (US)",
volume = "44",
pages = "600--604",
journal = "Chinese Journal of Cardiology",
issn = "0253-3758",
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TY - JOUR

T1 - Composition of potassium channels in normal rat coronary smooth muscle cells and activation effects of docosahexaenoic acid

AU - Qian, L. L.

AU - Wang, R. X.

AU - Sun, M. Q.

AU - Xia, D. Y.

AU - Tang, X.

AU - Ji, Y.

AU - Wu, Y.

AU - Liu, X. Y.

AU - Dang, S. P.

AU - Chai, Q.

AU - Lu, Tong D

PY - 2016/7/24

Y1 - 2016/7/24

N2 - RESULTS: Potassium currents were significantly increased with 5 μmol/L DHA perfusion (P<0.05). The current density was increased from (52.80±6.68) pA/pF to (110.09±13.39) pA/pF (P<0.05) after DHA perfusion when the stimulation voltage was 100 mV.Compared with baseline, potassium currents were significantly decreased by various inhibitor perfusion (tetraethylammonium: (49.63±5.75) pA/pF vs. (13.96±2.18) pA/pF; ibritoxin: (50.67±7.89) pA/pF vs. (26.53±4.68) pA/pF; TRAM-34: (52.60±7.02) pA/pF vs. (46.05±7.60) pA/pF; apamin: (51.97±3.83) pA/pF vs. (44.89±5.04) pA/pF; 4-aminopyridine: (51.19±3.44) pA/pF vs. (29.92±2.81) pA/pF; glyburide: (49.67±1.77) pA/pF vs. (49.61±1.87) pA/pF, all P<0.05). In presence of different inhibitors, potassium channel current densities were increased after DHA perfusion except tetraethylammonium (tetraethylammonium: ( 12.79±1.89) pA/pF; ibritoxin: (67.08±5.54) pA/pF; TRAM-34: (117.91±21.79) pA/pF; apamin: (108.33±7.06) pA/pF; 4-aminopyridine: (127.73±20.56) pA/pF; glyburide: (121.53±13.83) pA/pF, all P<0.05 compared with baseline).CONCLUSIONS: Large-conductance calcium-activated potassium channel and voltage-gated potassium channel are the major constituents of potassium channels in CASMCs.DHA can activate potassium channels in CASMCs, mainly the large conductance calcium-activated potassium channel, thus dilate coronary arteries.OBJECTIVE: To investigate the composition of potassium channels in normal rat coronary smooth muscle cells (CASMCs) and the activation effects of docosahexaenoic acid (DHA).METHODS: CASMCs were isolated by enzyme digestion.Effects of different types of potassium channel blockers and/or DHA on potassium channels currents were studied by whole-cell patch clamp technique.

AB - RESULTS: Potassium currents were significantly increased with 5 μmol/L DHA perfusion (P<0.05). The current density was increased from (52.80±6.68) pA/pF to (110.09±13.39) pA/pF (P<0.05) after DHA perfusion when the stimulation voltage was 100 mV.Compared with baseline, potassium currents were significantly decreased by various inhibitor perfusion (tetraethylammonium: (49.63±5.75) pA/pF vs. (13.96±2.18) pA/pF; ibritoxin: (50.67±7.89) pA/pF vs. (26.53±4.68) pA/pF; TRAM-34: (52.60±7.02) pA/pF vs. (46.05±7.60) pA/pF; apamin: (51.97±3.83) pA/pF vs. (44.89±5.04) pA/pF; 4-aminopyridine: (51.19±3.44) pA/pF vs. (29.92±2.81) pA/pF; glyburide: (49.67±1.77) pA/pF vs. (49.61±1.87) pA/pF, all P<0.05). In presence of different inhibitors, potassium channel current densities were increased after DHA perfusion except tetraethylammonium (tetraethylammonium: ( 12.79±1.89) pA/pF; ibritoxin: (67.08±5.54) pA/pF; TRAM-34: (117.91±21.79) pA/pF; apamin: (108.33±7.06) pA/pF; 4-aminopyridine: (127.73±20.56) pA/pF; glyburide: (121.53±13.83) pA/pF, all P<0.05 compared with baseline).CONCLUSIONS: Large-conductance calcium-activated potassium channel and voltage-gated potassium channel are the major constituents of potassium channels in CASMCs.DHA can activate potassium channels in CASMCs, mainly the large conductance calcium-activated potassium channel, thus dilate coronary arteries.OBJECTIVE: To investigate the composition of potassium channels in normal rat coronary smooth muscle cells (CASMCs) and the activation effects of docosahexaenoic acid (DHA).METHODS: CASMCs were isolated by enzyme digestion.Effects of different types of potassium channel blockers and/or DHA on potassium channels currents were studied by whole-cell patch clamp technique.

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