TY - JOUR
T1 - Composition of commercial media used for human embryo culture
AU - Morbeck, Dean E.
AU - Krisher, Rebecca L.
AU - Herrick, Jason R.
AU - Baumann, Nikola A.
AU - Matern, Dietrich
AU - Moyer, Thomas
N1 - Funding Information:
Supported by a grant from the Mayo Clinic Department of Laboratory Medicine and Pathology and an equipment loan (EmbryoScope) from Unisense Fertilitech.
PY - 2014/9
Y1 - 2014/9
N2 - Objective To determine the composition of commercially available culture media and test whether differences in composition are biologically relevant in a murine model. Design Experimental laboratory study. Setting University-based laboratory. Animal(s) Cryopreserved hybrid mouse one-cell embryos were used in experiments. Intervention(s) Amino acid, organic acid, ions, and metal content were determined for two different lots of media from Cook, In Vitro Care, Origio, Sage, Vitrolife, Irvine CSC, and Global. To determine whether differences in the composition of these media are biologically relevant, mouse one-cell embryos were thawed and cultured for 120 hours in each culture media at 5% and 20% oxygen in the presence or absence of protein in an EmbryoScope time-lapse incubator. Main Outcome Measure(s) The compositions of seven culture media were analyzed for concentrations of 39 individual amino acids, organic acids, ions, and elements. Blastocyst rates and cell cycle timings were calculated at 96 hours of culture, and the experiments were repeated in triplicate. Result(s) Of the 39 analytes, concentrations of glucose, lactate, pyruvate, amino acids, phosphate, calcium, and magnesium were present in variable concentrations, likely reflecting differences in the interpretation of animal studies. Essential trace elements, such as copper and zinc, were not detected. Mouse embryos failed to develop in one culture medium and were differentially affected by oxygen in two other media. Conclusion(s) Culture media composition varies widely, with differences in pyruvate, lactate, and amino acids especially notable. Blastocyst development was culture media dependent and showed an interaction with oxygen concentration and presence of protein.
AB - Objective To determine the composition of commercially available culture media and test whether differences in composition are biologically relevant in a murine model. Design Experimental laboratory study. Setting University-based laboratory. Animal(s) Cryopreserved hybrid mouse one-cell embryos were used in experiments. Intervention(s) Amino acid, organic acid, ions, and metal content were determined for two different lots of media from Cook, In Vitro Care, Origio, Sage, Vitrolife, Irvine CSC, and Global. To determine whether differences in the composition of these media are biologically relevant, mouse one-cell embryos were thawed and cultured for 120 hours in each culture media at 5% and 20% oxygen in the presence or absence of protein in an EmbryoScope time-lapse incubator. Main Outcome Measure(s) The compositions of seven culture media were analyzed for concentrations of 39 individual amino acids, organic acids, ions, and elements. Blastocyst rates and cell cycle timings were calculated at 96 hours of culture, and the experiments were repeated in triplicate. Result(s) Of the 39 analytes, concentrations of glucose, lactate, pyruvate, amino acids, phosphate, calcium, and magnesium were present in variable concentrations, likely reflecting differences in the interpretation of animal studies. Essential trace elements, such as copper and zinc, were not detected. Mouse embryos failed to develop in one culture medium and were differentially affected by oxygen in two other media. Conclusion(s) Culture media composition varies widely, with differences in pyruvate, lactate, and amino acids especially notable. Blastocyst development was culture media dependent and showed an interaction with oxygen concentration and presence of protein.
KW - Culture media
KW - embryo culture
KW - mouse embryo assay
KW - quality control
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U2 - 10.1016/j.fertnstert.2014.05.043
DO - 10.1016/j.fertnstert.2014.05.043
M3 - Article
C2 - 24998366
AN - SCOPUS:84906939333
SN - 0015-0282
VL - 102
SP - 759-766.e9
JO - Fertility and sterility
JF - Fertility and sterility
IS - 3
ER -