Complement component 3 (C3) haplotypes and risk of advanced age-related macular degeneration

Kyu Hyung Park, Brooke L. Fridley, Euijung Ryu, Nirubol Tosakulwong, Albert O. Edwards

Research output: Contribution to journalArticle

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Abstract

PURPOSE. Nonsynonymous coding single nucleotide polymorphisms (nsSNPs) in complement component 3 (C3) alter the risk of age-related macular degeneration (AMD). This was a study of the effect of haplotypes across C3 on AMD risk. METHODS. Nine SNPs tagging haplotypes across C3 were genotyped on 738 subjects at the Mayo Clinic. Haplotype analyses were performed with and without conditioning on individual SNPs. Replication studies were performed using 1541 subjects from the age-related eye disease study (AREDS). RESULTS. Two nsSNPs located 5125 bp apart in the 5' end of C3 showed the highest association (rs1047286 or P314L, P = 9.2E-05; rs2230199 or R102G, P = 4.1E-05) with AMD. The minor alleles of both SNPs tagged a single risk haplotype. The effects of the two nsSNPs could not be distinguished due to high linkage disequilibrium. The risk SNPs preferentially promoted the development of advanced AMD relative to early AMD in both the Mayo and AREDS subjects. Haplotypes in the 3' end of the C3 locus were associated with AMD in both the Mayo and AREDS subjects. The effect persisted after conditioning on the nsSNPs only in the Mayo subjects. No interaction was found between rs2230199 and smoking or other AMD loci. CONCLUSIONS. nsSNPs in C3 increased the risk of developing AMD 1.8-fold for 1 risk allele or 2.4-fold for two risk alleles and were preferentially associated with advanced AMD. Further study is needed to determine whether haplotypes in the 3' end of C3 have an independent association with AMD.

Original languageEnglish (US)
Pages (from-to)3386-3393
Number of pages8
JournalInvestigative Ophthalmology and Visual Science
Volume50
Issue number7
DOIs
StatePublished - Dec 1 2009

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Complement C3
Macular Degeneration
Haplotypes
Single Nucleotide Polymorphism
Eye Diseases
Alleles
Linkage Disequilibrium
Smoking

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

Complement component 3 (C3) haplotypes and risk of advanced age-related macular degeneration. / Park, Kyu Hyung; Fridley, Brooke L.; Ryu, Euijung; Tosakulwong, Nirubol; Edwards, Albert O.

In: Investigative Ophthalmology and Visual Science, Vol. 50, No. 7, 01.12.2009, p. 3386-3393.

Research output: Contribution to journalArticle

Park, Kyu Hyung ; Fridley, Brooke L. ; Ryu, Euijung ; Tosakulwong, Nirubol ; Edwards, Albert O. / Complement component 3 (C3) haplotypes and risk of advanced age-related macular degeneration. In: Investigative Ophthalmology and Visual Science. 2009 ; Vol. 50, No. 7. pp. 3386-3393.
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N2 - PURPOSE. Nonsynonymous coding single nucleotide polymorphisms (nsSNPs) in complement component 3 (C3) alter the risk of age-related macular degeneration (AMD). This was a study of the effect of haplotypes across C3 on AMD risk. METHODS. Nine SNPs tagging haplotypes across C3 were genotyped on 738 subjects at the Mayo Clinic. Haplotype analyses were performed with and without conditioning on individual SNPs. Replication studies were performed using 1541 subjects from the age-related eye disease study (AREDS). RESULTS. Two nsSNPs located 5125 bp apart in the 5' end of C3 showed the highest association (rs1047286 or P314L, P = 9.2E-05; rs2230199 or R102G, P = 4.1E-05) with AMD. The minor alleles of both SNPs tagged a single risk haplotype. The effects of the two nsSNPs could not be distinguished due to high linkage disequilibrium. The risk SNPs preferentially promoted the development of advanced AMD relative to early AMD in both the Mayo and AREDS subjects. Haplotypes in the 3' end of the C3 locus were associated with AMD in both the Mayo and AREDS subjects. The effect persisted after conditioning on the nsSNPs only in the Mayo subjects. No interaction was found between rs2230199 and smoking or other AMD loci. CONCLUSIONS. nsSNPs in C3 increased the risk of developing AMD 1.8-fold for 1 risk allele or 2.4-fold for two risk alleles and were preferentially associated with advanced AMD. Further study is needed to determine whether haplotypes in the 3' end of C3 have an independent association with AMD.

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