Comparison of visible and near-infrared wavelength-excitable fluorescent dyes for molecular imaging of cancer

Kristen E. Adams, Shi Ke, Sunkuk Kwon, Feng Liang, Zhen Fan, Yang Lu, Karen Hirschi, Michel E. Mawad, Michael A. Barry, Eva M. Sevick-Muraca

Research output: Contribution to journalArticle

174 Scopus citations

Abstract

Targeted fluorescent molecular imaging probes may provide an optimal means of detecting disease. Stable, organic fluorophores can be repeatedly excited in vivo by propagated light and consequentially can provide large signal-to-noise ratios (SNRs) for image detection of target tissues. In the literature, many small animal imaging studies are performed with a red excitable dye, Cy5.5, conjugated to the targeting component. We report the comparison of the in vivo fluorescent imaging performance of a near-IR (NIR) and a red-excitable dye. Epidermal growth factor (EGF) was conjugated with Cy5.5 [excitation/emission (ex/em), 660/710 nm] or IRDye® 800CW (ex/em: 785/830 nm) for imaging EGF receptor (EGFr) positive (MDA-MB-468) and/or negative (MDA-MB-435) human breast cancer cell lines in subcutaneous xenograft models. The conjugates were injected intravenously at 1-nmol-dye equivalent with and without anti- EGFr monoclonal antibody C225, preadministered 24 h prior as a competitive ligand to EGFr. Our images show that while both agents target EGFr, the EGF-IRDye® 800CW evidenced a significantly reduced background and enhanced the tumor-to-background ratio (TBR) compared to the EGF-Cy5.5. Immunohistochemistry shows that EGF causes activation of the EGFr signaling pathway, suggesting that prior to use as a targeting, diagnostic agent, potential deleterious effects should be considered.

Original languageEnglish (US)
Article number024017
JournalJournal of Biomedical Optics
Volume12
Issue number2
DOIs
StatePublished - Mar 1 2007

Keywords

  • Epidermal growth factor receptor
  • Molecular imaging
  • Near-infrared
  • Small animal

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Biomedical Engineering

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    Adams, K. E., Ke, S., Kwon, S., Liang, F., Fan, Z., Lu, Y., Hirschi, K., Mawad, M. E., Barry, M. A., & Sevick-Muraca, E. M. (2007). Comparison of visible and near-infrared wavelength-excitable fluorescent dyes for molecular imaging of cancer. Journal of Biomedical Optics, 12(2), [024017]. https://doi.org/10.1117/1.2717137