TY - JOUR
T1 - Comparison of three preparatory methods for detection of bacteremia by MALDI-TOF mass spectrometry
AU - Saffert, Ryan T.
AU - Cunningham, Scott A.
AU - Mandrekar, Jayawant
AU - Patel, Robin
PY - 2012/5
Y1 - 2012/5
N2 - We evaluated 3 preparatory methods for processing positive blood culture bottle broths prior to matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) analysis-differential centrifugation, 10% sodium dodecyl sulfate (SDS), and the Sepsityper kit. Initial evaluation used genus and species level cutoff scores of 1.700-1.999 and ≥2.000, respectively. Processing of an initial 79 blood bottle cultures by differential centrifugation yielded correct identifications of 51 (65%) to the genus and 34 (43%) to the species levels. One hundred and one different blood bottles were simultaneously processed with 10% SDS and Sepsityper. Both yielded genus-level identifications for 77 (76%), and the 2 yielded species-level identifications for 49 (49%) and 54 (54%) of bottles, respectively. Adjustment of the score cutoff criteria for genus (1.500-1.699) and species (≥1.700) improved identification percentages, particularly for species-level identifications (. P < 0.05).
AB - We evaluated 3 preparatory methods for processing positive blood culture bottle broths prior to matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) analysis-differential centrifugation, 10% sodium dodecyl sulfate (SDS), and the Sepsityper kit. Initial evaluation used genus and species level cutoff scores of 1.700-1.999 and ≥2.000, respectively. Processing of an initial 79 blood bottle cultures by differential centrifugation yielded correct identifications of 51 (65%) to the genus and 34 (43%) to the species levels. One hundred and one different blood bottles were simultaneously processed with 10% SDS and Sepsityper. Both yielded genus-level identifications for 77 (76%), and the 2 yielded species-level identifications for 49 (49%) and 54 (54%) of bottles, respectively. Adjustment of the score cutoff criteria for genus (1.500-1.699) and species (≥1.700) improved identification percentages, particularly for species-level identifications (. P < 0.05).
KW - Bloodstream infection
KW - MALDI-TOF
KW - Mass spectrometry
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U2 - 10.1016/j.diagmicrobio.2012.01.010
DO - 10.1016/j.diagmicrobio.2012.01.010
M3 - Article
C2 - 22578935
AN - SCOPUS:84860714032
SN - 0732-8893
VL - 73
SP - 21
EP - 26
JO - Diagnostic Microbiology and Infectious Disease
JF - Diagnostic Microbiology and Infectious Disease
IS - 1
ER -