Comparison of three preparatory methods for detection of bacteremia by MALDI-TOF mass spectrometry

Ryan T. Saffert, Scott A. Cunningham, Jayawant Mandrekar, Robin Patel

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

We evaluated 3 preparatory methods for processing positive blood culture bottle broths prior to matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) analysis-differential centrifugation, 10% sodium dodecyl sulfate (SDS), and the Sepsityper kit. Initial evaluation used genus and species level cutoff scores of 1.700-1.999 and ≥2.000, respectively. Processing of an initial 79 blood bottle cultures by differential centrifugation yielded correct identifications of 51 (65%) to the genus and 34 (43%) to the species levels. One hundred and one different blood bottles were simultaneously processed with 10% SDS and Sepsityper. Both yielded genus-level identifications for 77 (76%), and the 2 yielded species-level identifications for 49 (49%) and 54 (54%) of bottles, respectively. Adjustment of the score cutoff criteria for genus (1.500-1.699) and species (≥1.700) improved identification percentages, particularly for species-level identifications (. P < 0.05).

Original languageEnglish (US)
Pages (from-to)21-26
Number of pages6
JournalDiagnostic Microbiology and Infectious Disease
Volume73
Issue number1
DOIs
StatePublished - May 2012

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Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Bacteremia
Centrifugation
Sodium Dodecyl Sulfate
Mass Spectrometry
Lasers
Blood Culture

Keywords

  • Bloodstream infection
  • MALDI-TOF
  • Mass spectrometry

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

Cite this

Comparison of three preparatory methods for detection of bacteremia by MALDI-TOF mass spectrometry. / Saffert, Ryan T.; Cunningham, Scott A.; Mandrekar, Jayawant; Patel, Robin.

In: Diagnostic Microbiology and Infectious Disease, Vol. 73, No. 1, 05.2012, p. 21-26.

Research output: Contribution to journalArticle

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