Comparison of the expression and activity of phosphatidylethanolamine N-methyltransferase 2 between primary cultured hepatocytes and hepatoma cells in rats

W. Zou, Zhaoyu Li, Z. Cui

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

OBJECTIVE: To compare the expression and activity of phosphatidyl-ethanolamine N-methyltransferase 2 (PEMT(2)) between the primary cultured hepatocytes and CBRH7919 hepatoma cells in rats. METHODS: The expression and activity of PEMT(2) were demonstrated by immunochemistry, Western immunoblotting and [(3)H-CH(3)] SAM incorporation. Flow cytometry (FCM) was used to investigate the DNA content of the cells. RESULTS: PEMT(2) expression and activity were higher in the primary cultured hepatocytes than in hepatoma cells. Cell cycle analysis showed that the proportion of the hepatocytes was higher in G(1) phase and lower in S phase in the primary cultured hepatocytes than hepatoma cell lines. CONCLUSION: There is an intimate relationship between the expression of PEMT(2) and the proliferation of hepatocytes.

Original languageEnglish (US)
Pages (from-to)230-232
Number of pages3
JournalZhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology
Volume8
Issue number4
StatePublished - 2000
Externally publishedYes

Fingerprint

Phosphatidylethanolamine N-Methyltransferase
Ethanolamine
Hepatocytes
Hepatocellular Carcinoma
Immunochemistry
S Phase
Cell Cycle
Flow Cytometry
Western Blotting
Cell Line
methylcobalamin-coenzyme M methyltransferase
DNA

Cite this

@article{832ef05f3a8f42998c4b48bb4ebe2aae,
title = "Comparison of the expression and activity of phosphatidylethanolamine N-methyltransferase 2 between primary cultured hepatocytes and hepatoma cells in rats",
abstract = "OBJECTIVE: To compare the expression and activity of phosphatidyl-ethanolamine N-methyltransferase 2 (PEMT(2)) between the primary cultured hepatocytes and CBRH7919 hepatoma cells in rats. METHODS: The expression and activity of PEMT(2) were demonstrated by immunochemistry, Western immunoblotting and [(3)H-CH(3)] SAM incorporation. Flow cytometry (FCM) was used to investigate the DNA content of the cells. RESULTS: PEMT(2) expression and activity were higher in the primary cultured hepatocytes than in hepatoma cells. Cell cycle analysis showed that the proportion of the hepatocytes was higher in G(1) phase and lower in S phase in the primary cultured hepatocytes than hepatoma cell lines. CONCLUSION: There is an intimate relationship between the expression of PEMT(2) and the proliferation of hepatocytes.",
author = "W. Zou and Zhaoyu Li and Z. Cui",
year = "2000",
language = "English (US)",
volume = "8",
pages = "230--232",
journal = "Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology",
issn = "1007-3418",
publisher = "Zhonghua Yixuehui Zazhishe",
number = "4",

}

TY - JOUR

T1 - Comparison of the expression and activity of phosphatidylethanolamine N-methyltransferase 2 between primary cultured hepatocytes and hepatoma cells in rats

AU - Zou, W.

AU - Li, Zhaoyu

AU - Cui, Z.

PY - 2000

Y1 - 2000

N2 - OBJECTIVE: To compare the expression and activity of phosphatidyl-ethanolamine N-methyltransferase 2 (PEMT(2)) between the primary cultured hepatocytes and CBRH7919 hepatoma cells in rats. METHODS: The expression and activity of PEMT(2) were demonstrated by immunochemistry, Western immunoblotting and [(3)H-CH(3)] SAM incorporation. Flow cytometry (FCM) was used to investigate the DNA content of the cells. RESULTS: PEMT(2) expression and activity were higher in the primary cultured hepatocytes than in hepatoma cells. Cell cycle analysis showed that the proportion of the hepatocytes was higher in G(1) phase and lower in S phase in the primary cultured hepatocytes than hepatoma cell lines. CONCLUSION: There is an intimate relationship between the expression of PEMT(2) and the proliferation of hepatocytes.

AB - OBJECTIVE: To compare the expression and activity of phosphatidyl-ethanolamine N-methyltransferase 2 (PEMT(2)) between the primary cultured hepatocytes and CBRH7919 hepatoma cells in rats. METHODS: The expression and activity of PEMT(2) were demonstrated by immunochemistry, Western immunoblotting and [(3)H-CH(3)] SAM incorporation. Flow cytometry (FCM) was used to investigate the DNA content of the cells. RESULTS: PEMT(2) expression and activity were higher in the primary cultured hepatocytes than in hepatoma cells. Cell cycle analysis showed that the proportion of the hepatocytes was higher in G(1) phase and lower in S phase in the primary cultured hepatocytes than hepatoma cell lines. CONCLUSION: There is an intimate relationship between the expression of PEMT(2) and the proliferation of hepatocytes.

UR - http://www.scopus.com/inward/record.url?scp=0034241470&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034241470&partnerID=8YFLogxK

M3 - Article

VL - 8

SP - 230

EP - 232

JO - Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology

JF - Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology

SN - 1007-3418

IS - 4

ER -