Comparison of the diagnostic utility of digital pathology systems for telemicrobiology

Daniel D. Rhoads, Nadia F. Habib-Bein, Rahman S. Hariri, Douglas J. Hartman, Sara E. Monaco, Andrew Lesniak, Jon Duboy, Mohamed Salama, Liron Pantanowitz

Research output: Contribution to journalArticle

4 Scopus citations


Introduction: Telemicrobiology is a growing component of clinical microbiology informatics. However, few studies have been performed to assess the diagnostic utility of telemicroscopy systems in evaluating infectious agents. Objective: Evaluate multiple contemporary digital pathology platforms for use in diagnostic telemicrobiology. Materials and Methods: A mix of thirty cases that included viral, bacterial, fungal, and parasitological findings were evaluated by four experts using ×40 whole slide imaging (WSI) scans, ×83 oil-immersion WSI scans, ×100 oil-immersion WSI scans, digital photomicrographs, and glass slides. Results: The ×83 WSI, ×100 WSI, and photomicrograph interpretations were not significantly different in quality and accuracy when compared to glass slide interpretations. The ×40 WSI interpretations were of lower quality and were more likely to be incorrect when compared to glass slide interpretations. Conclusions: In this study, high magnification, oil-immersion digital pathology platforms are better suited to support telemicrobiology applications and yield interpretations on par with glass slide evaluations.

Original languageEnglish (US)
Article number10
JournalJournal of Pathology Informatics
Issue number1
StatePublished - Jan 29 2016
Externally publishedYes



  • Digital pathology
  • infectious diseases
  • microbiology
  • telemicrobiology
  • telemicroscopy
  • whole slide imaging

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Health Informatics
  • Computer Science Applications

Cite this

Rhoads, D. D., Habib-Bein, N. F., Hariri, R. S., Hartman, D. J., Monaco, S. E., Lesniak, A., Duboy, J., Salama, M., & Pantanowitz, L. (2016). Comparison of the diagnostic utility of digital pathology systems for telemicrobiology. Journal of Pathology Informatics, 7(1), [10].