Comparison of telomerase and CD44 expression as diagnostic tumor markers in lesions of the thyroid

Kenjiro Aogi, Kenji Kitahara, Virginia Urquidi, David Tarin, Steven Goodison

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

The analysis of the tissue expression patterns of both the telomerase enzyme and the adhesion molecule CD44 has highlighted these molecules as potential tumor markers. In this study, the expression of these markers was analyzed in frozen tissue samples of the same human thyroid lesions, and the data were compared to evaluate their application to tumor diagnosis. The study analyzed 12 malignant specimens, including 5 papillary, 3 follicular, 2 anaplastic, 1 medullary, and 1 low-grade Hurthle cell carcinoma and 17 specimens from benign lesions, including cases of adenoma, hyperplasia, and Graves' disease. Telomerase expression was analyzed by assay of enzyme activity using the telomeric repeat amplification protocol and by reverse transcription-PCR detection of human telomerase reverse transcriptase (hTERT) mRNA. Nine of 12 (75%) malignant samples and the two Graves' disease samples were evaluated as positive for telomerase activity by the telomeric repeat amplification protocol assay. The presence of hTERT mRNA was detected in 8 (67%) of 12 malignant tissues and in 5 (29%) of 17 benign thyroid tissue samples. The expression of CD44 transcripts containing variant exons 7, 8, and 11 was evaluated by reverse transcription-PCR/Southern blot analysis. Of the 12 malignant samples, 9 (75 %) included transcripts containing exon 7, 10 (83%) included transcripts containing exon 11, and 11 (92%) included transcripts containing exon 8. However, these CD44 exons were also present in transcripts in a high proportion of benign samples. Five (28%), 10 (59%), and 6 (35%) benign samples contained CD44 transcripts, including variant exons 7, 8, and 11, respectively. The measurement of telomerase activity proved to be the most specific for the detection of thyroid carcinoma in frozen tissue samples as a single analyze, but diagnostic accuracy was increased by the combination of telomerase and CD44 analyses.

Original languageEnglish (US)
Pages (from-to)2790-2797
Number of pages8
JournalClinical Cancer Research
Volume5
Issue number10
StatePublished - Oct 1 1999
Externally publishedYes

Fingerprint

Telomerase
Tumor Biomarkers
Exons
Thyroid Gland
Graves Disease
Reverse Transcription
Oxyphil Cells
Polymerase Chain Reaction
Messenger RNA
Enzyme Assays
Southern Blotting
Thyroid Neoplasms
Adenoma
Hyperplasia
Carcinoma
Enzymes
Neoplasms

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Comparison of telomerase and CD44 expression as diagnostic tumor markers in lesions of the thyroid. / Aogi, Kenjiro; Kitahara, Kenji; Urquidi, Virginia; Tarin, David; Goodison, Steven.

In: Clinical Cancer Research, Vol. 5, No. 10, 01.10.1999, p. 2790-2797.

Research output: Contribution to journalArticle

Aogi, Kenjiro ; Kitahara, Kenji ; Urquidi, Virginia ; Tarin, David ; Goodison, Steven. / Comparison of telomerase and CD44 expression as diagnostic tumor markers in lesions of the thyroid. In: Clinical Cancer Research. 1999 ; Vol. 5, No. 10. pp. 2790-2797.
@article{0022b849d6db435a8ac194584011f88a,
title = "Comparison of telomerase and CD44 expression as diagnostic tumor markers in lesions of the thyroid",
abstract = "The analysis of the tissue expression patterns of both the telomerase enzyme and the adhesion molecule CD44 has highlighted these molecules as potential tumor markers. In this study, the expression of these markers was analyzed in frozen tissue samples of the same human thyroid lesions, and the data were compared to evaluate their application to tumor diagnosis. The study analyzed 12 malignant specimens, including 5 papillary, 3 follicular, 2 anaplastic, 1 medullary, and 1 low-grade Hurthle cell carcinoma and 17 specimens from benign lesions, including cases of adenoma, hyperplasia, and Graves' disease. Telomerase expression was analyzed by assay of enzyme activity using the telomeric repeat amplification protocol and by reverse transcription-PCR detection of human telomerase reverse transcriptase (hTERT) mRNA. Nine of 12 (75{\%}) malignant samples and the two Graves' disease samples were evaluated as positive for telomerase activity by the telomeric repeat amplification protocol assay. The presence of hTERT mRNA was detected in 8 (67{\%}) of 12 malignant tissues and in 5 (29{\%}) of 17 benign thyroid tissue samples. The expression of CD44 transcripts containing variant exons 7, 8, and 11 was evaluated by reverse transcription-PCR/Southern blot analysis. Of the 12 malignant samples, 9 (75 {\%}) included transcripts containing exon 7, 10 (83{\%}) included transcripts containing exon 11, and 11 (92{\%}) included transcripts containing exon 8. However, these CD44 exons were also present in transcripts in a high proportion of benign samples. Five (28{\%}), 10 (59{\%}), and 6 (35{\%}) benign samples contained CD44 transcripts, including variant exons 7, 8, and 11, respectively. The measurement of telomerase activity proved to be the most specific for the detection of thyroid carcinoma in frozen tissue samples as a single analyze, but diagnostic accuracy was increased by the combination of telomerase and CD44 analyses.",
author = "Kenjiro Aogi and Kenji Kitahara and Virginia Urquidi and David Tarin and Steven Goodison",
year = "1999",
month = "10",
day = "1",
language = "English (US)",
volume = "5",
pages = "2790--2797",
journal = "Clinical Cancer Research",
issn = "1078-0432",
publisher = "American Association for Cancer Research Inc.",
number = "10",

}

TY - JOUR

T1 - Comparison of telomerase and CD44 expression as diagnostic tumor markers in lesions of the thyroid

AU - Aogi, Kenjiro

AU - Kitahara, Kenji

AU - Urquidi, Virginia

AU - Tarin, David

AU - Goodison, Steven

PY - 1999/10/1

Y1 - 1999/10/1

N2 - The analysis of the tissue expression patterns of both the telomerase enzyme and the adhesion molecule CD44 has highlighted these molecules as potential tumor markers. In this study, the expression of these markers was analyzed in frozen tissue samples of the same human thyroid lesions, and the data were compared to evaluate their application to tumor diagnosis. The study analyzed 12 malignant specimens, including 5 papillary, 3 follicular, 2 anaplastic, 1 medullary, and 1 low-grade Hurthle cell carcinoma and 17 specimens from benign lesions, including cases of adenoma, hyperplasia, and Graves' disease. Telomerase expression was analyzed by assay of enzyme activity using the telomeric repeat amplification protocol and by reverse transcription-PCR detection of human telomerase reverse transcriptase (hTERT) mRNA. Nine of 12 (75%) malignant samples and the two Graves' disease samples were evaluated as positive for telomerase activity by the telomeric repeat amplification protocol assay. The presence of hTERT mRNA was detected in 8 (67%) of 12 malignant tissues and in 5 (29%) of 17 benign thyroid tissue samples. The expression of CD44 transcripts containing variant exons 7, 8, and 11 was evaluated by reverse transcription-PCR/Southern blot analysis. Of the 12 malignant samples, 9 (75 %) included transcripts containing exon 7, 10 (83%) included transcripts containing exon 11, and 11 (92%) included transcripts containing exon 8. However, these CD44 exons were also present in transcripts in a high proportion of benign samples. Five (28%), 10 (59%), and 6 (35%) benign samples contained CD44 transcripts, including variant exons 7, 8, and 11, respectively. The measurement of telomerase activity proved to be the most specific for the detection of thyroid carcinoma in frozen tissue samples as a single analyze, but diagnostic accuracy was increased by the combination of telomerase and CD44 analyses.

AB - The analysis of the tissue expression patterns of both the telomerase enzyme and the adhesion molecule CD44 has highlighted these molecules as potential tumor markers. In this study, the expression of these markers was analyzed in frozen tissue samples of the same human thyroid lesions, and the data were compared to evaluate their application to tumor diagnosis. The study analyzed 12 malignant specimens, including 5 papillary, 3 follicular, 2 anaplastic, 1 medullary, and 1 low-grade Hurthle cell carcinoma and 17 specimens from benign lesions, including cases of adenoma, hyperplasia, and Graves' disease. Telomerase expression was analyzed by assay of enzyme activity using the telomeric repeat amplification protocol and by reverse transcription-PCR detection of human telomerase reverse transcriptase (hTERT) mRNA. Nine of 12 (75%) malignant samples and the two Graves' disease samples were evaluated as positive for telomerase activity by the telomeric repeat amplification protocol assay. The presence of hTERT mRNA was detected in 8 (67%) of 12 malignant tissues and in 5 (29%) of 17 benign thyroid tissue samples. The expression of CD44 transcripts containing variant exons 7, 8, and 11 was evaluated by reverse transcription-PCR/Southern blot analysis. Of the 12 malignant samples, 9 (75 %) included transcripts containing exon 7, 10 (83%) included transcripts containing exon 11, and 11 (92%) included transcripts containing exon 8. However, these CD44 exons were also present in transcripts in a high proportion of benign samples. Five (28%), 10 (59%), and 6 (35%) benign samples contained CD44 transcripts, including variant exons 7, 8, and 11, respectively. The measurement of telomerase activity proved to be the most specific for the detection of thyroid carcinoma in frozen tissue samples as a single analyze, but diagnostic accuracy was increased by the combination of telomerase and CD44 analyses.

UR - http://www.scopus.com/inward/record.url?scp=0032719044&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032719044&partnerID=8YFLogxK

M3 - Article

C2 - 10537343

AN - SCOPUS:0032719044

VL - 5

SP - 2790

EP - 2797

JO - Clinical Cancer Research

JF - Clinical Cancer Research

SN - 1078-0432

IS - 10

ER -