Comparison of telomerase activity and GSTP1 promoter methylation in ejaculate as potential screening tests for prostate cancer

C. I. Suh, T. Shanafelt, D. J. May, K. R. Shroyer, J. B. Bobak, E. D. Crawford, G. J. Miller, N. Markham, L. M. Glode

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

New diagnostic tools are needed for the early detection of prostatic cancer. The molecular detection of prostate cancer cells in ejaculates was evaluated using complementary PCR-based methods. LNCaP cells, a cell line derived from prostatic carcinoma, were spiked into normal seminal ejaculates and the prostatic epithelial component of the specimens was isolated by immunomagnetic bead sorting, using a monoclonal antibody to prostate-specific membrane antigen (PSMA). Ejaculates from nine patients with a recent diagnosis of prostate cancer were processed in a similar fashion, using LNCaP-spiked aliquots as an internal positive control. Telomerase expression was evaluated by the telomeric repeat amplification protocol (TRAP) and glutathione S-transferase gene promoter (GSTP1) hypermethylation was evaluated by methylation-sensitive restriction endonuclease digestion and PCR amplification. Telomerase activity was detected in LNCaP cells recovered from normal seminal ejaculates but was not found in all nine samples from patients with prostate cancer. The sensitivity of GSTP1 analysis was similar to telomerase analysis for the detection of LNCaP cells from normal ejaculate samples but was positive in ejaculates from four out of nine patients with prostate cancer. GSTP1 DNA methylation status is more sensitive than telomerase analysis for the detection of malignant cells in seminal ejaculates from patients with prostate cancer (C) 2000 Academic Press.

Original languageEnglish (US)
Pages (from-to)211-217
Number of pages7
JournalMolecular and Cellular Probes
Volume14
Issue number4
DOIs
StatePublished - 2000
Externally publishedYes

Fingerprint

Telomerase
Methylation
Prostatic Neoplasms
Polymerase Chain Reaction
DNA Restriction Enzymes
DNA Methylation
Glutathione Transferase
Early Detection of Cancer
Digestion
Monoclonal Antibodies
Carcinoma
Cell Line
Genes

Keywords

  • Early detection
  • Ejaculate
  • GSTP1
  • Prostate cancer
  • Telomerase

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Suh, C. I., Shanafelt, T., May, D. J., Shroyer, K. R., Bobak, J. B., Crawford, E. D., ... Glode, L. M. (2000). Comparison of telomerase activity and GSTP1 promoter methylation in ejaculate as potential screening tests for prostate cancer. Molecular and Cellular Probes, 14(4), 211-217. https://doi.org/10.1006/mcpr.2000.0307

Comparison of telomerase activity and GSTP1 promoter methylation in ejaculate as potential screening tests for prostate cancer. / Suh, C. I.; Shanafelt, T.; May, D. J.; Shroyer, K. R.; Bobak, J. B.; Crawford, E. D.; Miller, G. J.; Markham, N.; Glode, L. M.

In: Molecular and Cellular Probes, Vol. 14, No. 4, 2000, p. 211-217.

Research output: Contribution to journalArticle

Suh, CI, Shanafelt, T, May, DJ, Shroyer, KR, Bobak, JB, Crawford, ED, Miller, GJ, Markham, N & Glode, LM 2000, 'Comparison of telomerase activity and GSTP1 promoter methylation in ejaculate as potential screening tests for prostate cancer', Molecular and Cellular Probes, vol. 14, no. 4, pp. 211-217. https://doi.org/10.1006/mcpr.2000.0307
Suh, C. I. ; Shanafelt, T. ; May, D. J. ; Shroyer, K. R. ; Bobak, J. B. ; Crawford, E. D. ; Miller, G. J. ; Markham, N. ; Glode, L. M. / Comparison of telomerase activity and GSTP1 promoter methylation in ejaculate as potential screening tests for prostate cancer. In: Molecular and Cellular Probes. 2000 ; Vol. 14, No. 4. pp. 211-217.
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