Comparison of specimen processing and nucleic acid extraction by the swab extraction tube system versus the MagNA pure LC system for laboratory diagnosis of herpes simplex virus infections by LightCycler PCR

N. C. Issa, M. J. Espy, J. R. Uhl, W. S. Harmsen, J. N. Mandrekar, R. E. Gullerud, M. D. Davis, T. F. Smith

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

A total of 563 specimens (234 dermal and 329 genital swabs) from patients suspected of having herpes simplex virus (HSV) infections were processed using two different extraction methods (the MagNA Pure LC system and the swab extraction tube system [SETS]); HSV DNA was amplified by LightCycler PCR. HSV DNA was detected in 157 of 563 specimens (27.9%) processed by the MagNA Pure LC system and in 179 of 563 specimens (31.8%) processed by SETS (P < 0.0001). There was no specimen processed by the MagNA Pure LC extraction method that was positive only for HSV DNA. Of 157 specimens positive by both methods, HSV DNA copy levels were higher (using cycle crossover points [cycle threshold {C T}]) with SETS (mean CT, 25.9 cycles) than with the MagNA Pure LC system (mean CT,32.0 cycles) (P < 0.0001). The time to process 32 samples was longer with the MagNA Pure LC extraction system (90 min) than with SETS (35 min). HSV DNA extraction using SETS is faster, less expensive, and more sensitive than the MagNA Pure LC system and could replace the latter for the laboratory diagnosis of HSV infections using LightCycler PCR.

Original languageEnglish (US)
Pages (from-to)1059-1063
Number of pages5
JournalJournal of clinical microbiology
Volume43
Issue number3
DOIs
StatePublished - Mar 2005

ASJC Scopus subject areas

  • Microbiology (medical)

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