Comparison of four commercial urinary albumin (microalbumin) methods: Implications for detecting diabetic nephropathy using random urine specimens

William L. Roberts, Clay B. Calcote, Curtiss B. Cook, Douglas L. Gordon, Marcia L. Moore, Spring Moore, W. Douglas Scheer, Beatrice A. Snazelle

Research output: Contribution to journalArticle

28 Scopus citations

Abstract

The results of four urinary albumin methods used to identify patients with early diabetic renal disease were compared using random urine samples from healthy and diabetic patients. These methods were the Beckman Array and Behring BNAI immunonephelometric methods, the Dade aca particle-enhanced turbidimetric inhibition immunoassay method, and the INCSTAR SPQ immunoturbidimetric method. The albumin/creatinine ratio reference interval was found to be 2-20 mg albumin/g creatinine (mg/g) for the Array and 3.5-27.5 mg/g for the aca method. All four methods were compared using urines from a group of diabetic and nondiabetic patients. The BNAI, SPQ and Array methods compared well with one another while the aca demonstrated a positive bias of almost 60% at the 30 mg/g and 300 mg/g levels with certain lots of reagent and calibrator. Calibrator cross-over experiments demonstrated that some of the positive bias of the aca method could be accounted for by calibrator differences. Copyright (C) 1998 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)21-33
Number of pages13
JournalClinica Chimica Acta
Volume273
Issue number1
DOIs
StatePublished - May 8 1998

Keywords

  • Albumin/creatinine ratio
  • Analytical methods
  • Diabetic nephropathy
  • Method bias
  • Microalbumin
  • Urinary albumin

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Biochemistry, medical

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    Roberts, W. L., Calcote, C. B., Cook, C. B., Gordon, D. L., Moore, M. L., Moore, S., Scheer, W. D., & Snazelle, B. A. (1998). Comparison of four commercial urinary albumin (microalbumin) methods: Implications for detecting diabetic nephropathy using random urine specimens. Clinica Chimica Acta, 273(1), 21-33. https://doi.org/10.1016/S0009-8981(98)00021-7