Comparison of fluorescence in situ hybridization, p57 immunostaining, flow cytometry, and digital image analysis for diagnosing molar and nonmolar products of conception

Benjamin R. Kipp; Rhett P. Ketterling; Trynda N. Oberg; Margot A. Cousin; Amy M. Plagge; Anne E. Wiktor; Johnita M. Ihrke; Cecelia H. Meyers; William G. Morice; Kevin C. Halling; Amy C. Clayton

doi:10.1309/AJCPV7BRDUCX0WAQ

Comparison of fluorescence in situ hybridization, p57 immunostaining, flow cytometry, and digital image analysis for diagnosing molar and nonmolar products of conception

Benjamin R. Kipp, Rhett P. Ketterling, Trynda N. Oberg, Margot A. Cousin, Amy M. Plagge, Anne E. Wiktor, Johnita M. Ihrke, Cecelia H. Meyers, William G. Morice, Kevin C. Halling, Amy C. Clayton

Laboratory Medicine and Pathology

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

Pathologic examination of products of conception (POC) is used to differentiate hydropic abortus (HA), partial hydatidiform mole (PM), and complete hydatidiform mole (CM). Histologic classification of POC specimens can be difficult, and ancillary testing is often required for a definitive diagnosis. This study evaluated 66 POC specimens by flow cytometry, digital image analysis, p57 immunohistochemical analysis, and fluorescence in situ hybridization (FISH). The final diagnosis, based on the combined analysis of all test results, included 33 HAs, 24 PMs, and 9 CMs. The p57 immunostain identified 9 CMs that were evaluated as nontriploid by all other techniques. FISH seems to have the best accuracy (100%) for determining whether a specimen contains a triploid chromosome complement. These data suggest that the combination of p57 and FISH seems to be the best ancillary testing strategy to aid pathologists in the appropriate identification of CM, PM, and HA in POC specimens.

Original language	English (US)
Pages (from-to)	196-204
Number of pages	9
Journal	American journal of clinical pathology
Volume	133
Issue number	2
DOIs	https://doi.org/10.1309/AJCPV7BRDUCX0WAQ
State	Published - Feb 2010

Keywords

DNA ploidy
FISH
Fluorescence in situ hybridization
Gestational trophoblastic disease
Hydropic abortus
Products of conception

ASJC Scopus subject areas

Pathology and Forensic Medicine

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10.1309/AJCPV7BRDUCX0WAQ

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Kipp, B. R., Ketterling, R. P., Oberg, T. N., Cousin, M. A., Plagge, A. M., Wiktor, A. E., Ihrke, J. M., Meyers, C. H., Morice, W. G., Halling, K. C., & Clayton, A. C. (2010). Comparison of fluorescence in situ hybridization, p57 immunostaining, flow cytometry, and digital image analysis for diagnosing molar and nonmolar products of conception. American journal of clinical pathology, 133(2), 196-204. https://doi.org/10.1309/AJCPV7BRDUCX0WAQ

Comparison of fluorescence in situ hybridization, p57 immunostaining, flow cytometry, and digital image analysis for diagnosing molar and nonmolar products of conception. / Kipp, Benjamin R.; Ketterling, Rhett P.; Oberg, Trynda N. et al.
In: American journal of clinical pathology, Vol. 133, No. 2, 02.2010, p. 196-204.

Research output: Contribution to journal › Article › peer-review

Kipp, BR, Ketterling, RP, Oberg, TN, Cousin, MA, Plagge, AM, Wiktor, AE, Ihrke, JM, Meyers, CH, Morice, WG, Halling, KC & Clayton, AC 2010, 'Comparison of fluorescence in situ hybridization, p57 immunostaining, flow cytometry, and digital image analysis for diagnosing molar and nonmolar products of conception', American journal of clinical pathology, vol. 133, no. 2, pp. 196-204. https://doi.org/10.1309/AJCPV7BRDUCX0WAQ

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abstract = "Pathologic examination of products of conception (POC) is used to differentiate hydropic abortus (HA), partial hydatidiform mole (PM), and complete hydatidiform mole (CM). Histologic classification of POC specimens can be difficult, and ancillary testing is often required for a definitive diagnosis. This study evaluated 66 POC specimens by flow cytometry, digital image analysis, p57 immunohistochemical analysis, and fluorescence in situ hybridization (FISH). The final diagnosis, based on the combined analysis of all test results, included 33 HAs, 24 PMs, and 9 CMs. The p57 immunostain identified 9 CMs that were evaluated as nontriploid by all other techniques. FISH seems to have the best accuracy (100%) for determining whether a specimen contains a triploid chromosome complement. These data suggest that the combination of p57 and FISH seems to be the best ancillary testing strategy to aid pathologists in the appropriate identification of CM, PM, and HA in POC specimens.",

keywords = "DNA ploidy, FISH, Fluorescence in situ hybridization, Gestational trophoblastic disease, Hydropic abortus, Products of conception",

author = "Kipp, {Benjamin R.} and Ketterling, {Rhett P.} and Oberg, {Trynda N.} and Cousin, {Margot A.} and Plagge, {Amy M.} and Wiktor, {Anne E.} and Ihrke, {Johnita M.} and Meyers, {Cecelia H.} and Morice, {William G.} and Halling, {Kevin C.} and Clayton, {Amy C.}",

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AU - Wiktor, Anne E.

AU - Ihrke, Johnita M.

AU - Meyers, Cecelia H.

AU - Morice, William G.

AU - Halling, Kevin C.

AU - Clayton, Amy C.

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AB - Pathologic examination of products of conception (POC) is used to differentiate hydropic abortus (HA), partial hydatidiform mole (PM), and complete hydatidiform mole (CM). Histologic classification of POC specimens can be difficult, and ancillary testing is often required for a definitive diagnosis. This study evaluated 66 POC specimens by flow cytometry, digital image analysis, p57 immunohistochemical analysis, and fluorescence in situ hybridization (FISH). The final diagnosis, based on the combined analysis of all test results, included 33 HAs, 24 PMs, and 9 CMs. The p57 immunostain identified 9 CMs that were evaluated as nontriploid by all other techniques. FISH seems to have the best accuracy (100%) for determining whether a specimen contains a triploid chromosome complement. These data suggest that the combination of p57 and FISH seems to be the best ancillary testing strategy to aid pathologists in the appropriate identification of CM, PM, and HA in POC specimens.

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Comparison of fluorescence in situ hybridization, p57 immunostaining, flow cytometry, and digital image analysis for diagnosing molar and nonmolar products of conception

Abstract

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