Comparison of fluorescence in situ hybridization, hybrid capture 2 and polymerase chain reaction for the detection of high-risk human papillomavirus in cervical cytology specimens

Jesse S. Voss, Benjamin R. Kipp, Michael B. Campion, Irina A. Sokolova, Michael R. Henry, Kevin C. Halling, Amy C. Clayton

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

OBJECTIVE: To compare a recently developed fluorescence in situ hybridization (FISH) high-risk human papillomavirus (HR-HPV) assay to Hybrid Capture 2 (HC2) (Digene Corporation, Gaithersburg, Maryland, U.S.A.) and polymerase chain reaction (PCR) for the detection of HR-HPV subtypes in cervical cytology specimens. STUDY DESIGN: One hundred forty-one liquid-based cytology specimens were used to produce a thin-layer slide for FISH analysis. The remaining material was sent for HC2 and PCR HR-HPV testing. Thin-layer slides were hybridized with a FISH probe set containing a biotin-labeled HR-HPV cocktail and were manually screened for HR-HPV-infected cells. Specimens with ≥ 1 HPV-positive cell by FISH were considered positive for HR-HPV infection. RESULTS: There was complete concordance between HC2, FISH and PCR in 104 (75%) specimens. FISH was concordant with HC2 and PCR in 120 (85%) and 115 (82%) specimens, respectively. HC2 and PCR were concordant in 118 (84%) specimens. CONCLUSION: The concordance of HR-HPV detection between FISH and HC2/PCR appears similar to concordances between HC2 and PCR. This suggests that FISH may be another method of detecting HR-HPV while having the potential to add additional information such as integrated/episomal staining and the ability to detect chromosomal abnormalities in individual cells.

Original languageEnglish (US)
Pages (from-to)208-216
Number of pages9
JournalAnalytical and Quantitative Cytology and Histology
Volume31
Issue number4
StatePublished - Aug 2009

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Fluorescence In Situ Hybridization
Cell Biology
Polymerase Chain Reaction
Papillomavirus Infections
Biotin
Chromosome Aberrations
Staining and Labeling

Keywords

  • Cervical neoplasms
  • Fluorescence
  • Human papillomavirus
  • In situ hybridization
  • Polymerase chain reaction

ASJC Scopus subject areas

  • Anatomy
  • Histology

Cite this

Comparison of fluorescence in situ hybridization, hybrid capture 2 and polymerase chain reaction for the detection of high-risk human papillomavirus in cervical cytology specimens. / Voss, Jesse S.; Kipp, Benjamin R.; Campion, Michael B.; Sokolova, Irina A.; Henry, Michael R.; Halling, Kevin C.; Clayton, Amy C.

In: Analytical and Quantitative Cytology and Histology, Vol. 31, No. 4, 08.2009, p. 208-216.

Research output: Contribution to journalArticle

Voss, Jesse S. ; Kipp, Benjamin R. ; Campion, Michael B. ; Sokolova, Irina A. ; Henry, Michael R. ; Halling, Kevin C. ; Clayton, Amy C. / Comparison of fluorescence in situ hybridization, hybrid capture 2 and polymerase chain reaction for the detection of high-risk human papillomavirus in cervical cytology specimens. In: Analytical and Quantitative Cytology and Histology. 2009 ; Vol. 31, No. 4. pp. 208-216.
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abstract = "OBJECTIVE: To compare a recently developed fluorescence in situ hybridization (FISH) high-risk human papillomavirus (HR-HPV) assay to Hybrid Capture 2 (HC2) (Digene Corporation, Gaithersburg, Maryland, U.S.A.) and polymerase chain reaction (PCR) for the detection of HR-HPV subtypes in cervical cytology specimens. STUDY DESIGN: One hundred forty-one liquid-based cytology specimens were used to produce a thin-layer slide for FISH analysis. The remaining material was sent for HC2 and PCR HR-HPV testing. Thin-layer slides were hybridized with a FISH probe set containing a biotin-labeled HR-HPV cocktail and were manually screened for HR-HPV-infected cells. Specimens with ≥ 1 HPV-positive cell by FISH were considered positive for HR-HPV infection. RESULTS: There was complete concordance between HC2, FISH and PCR in 104 (75{\%}) specimens. FISH was concordant with HC2 and PCR in 120 (85{\%}) and 115 (82{\%}) specimens, respectively. HC2 and PCR were concordant in 118 (84{\%}) specimens. CONCLUSION: The concordance of HR-HPV detection between FISH and HC2/PCR appears similar to concordances between HC2 and PCR. This suggests that FISH may be another method of detecting HR-HPV while having the potential to add additional information such as integrated/episomal staining and the ability to detect chromosomal abnormalities in individual cells.",
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AU - Henry, Michael R.

AU - Halling, Kevin C.

AU - Clayton, Amy C.

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AB - OBJECTIVE: To compare a recently developed fluorescence in situ hybridization (FISH) high-risk human papillomavirus (HR-HPV) assay to Hybrid Capture 2 (HC2) (Digene Corporation, Gaithersburg, Maryland, U.S.A.) and polymerase chain reaction (PCR) for the detection of HR-HPV subtypes in cervical cytology specimens. STUDY DESIGN: One hundred forty-one liquid-based cytology specimens were used to produce a thin-layer slide for FISH analysis. The remaining material was sent for HC2 and PCR HR-HPV testing. Thin-layer slides were hybridized with a FISH probe set containing a biotin-labeled HR-HPV cocktail and were manually screened for HR-HPV-infected cells. Specimens with ≥ 1 HPV-positive cell by FISH were considered positive for HR-HPV infection. RESULTS: There was complete concordance between HC2, FISH and PCR in 104 (75%) specimens. FISH was concordant with HC2 and PCR in 120 (85%) and 115 (82%) specimens, respectively. HC2 and PCR were concordant in 118 (84%) specimens. CONCLUSION: The concordance of HR-HPV detection between FISH and HC2/PCR appears similar to concordances between HC2 and PCR. This suggests that FISH may be another method of detecting HR-HPV while having the potential to add additional information such as integrated/episomal staining and the ability to detect chromosomal abnormalities in individual cells.

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