TY - JOUR
T1 - Comparison of cross-bridge cycling kinetics in neonatal vs. adult rat ventricular muscle
AU - Prakash, Y. S.
AU - Cody, Mark J.
AU - Housmans, Philippe R.
AU - Hannon, James D.
AU - Sieck, Gary C.
N1 - Funding Information:
This work is supported by NIH grants GM 57816 (Y.S.P.), GM 36365 (P.R.H.), and GM 57891 (F.D.H.), and the Mayo Foundation.
PY - 1999
Y1 - 1999
N2 - The developmental shift in contractile protein isoform expression in the rodent heart likely affects actin-myosin cross-bridge interactions. We compared the Ca2+ sensitivity for force generation and cross-bridge cycling kinetics in neonatal (postnatal days 0-3) and adult (day 84) rats. The force-pCa relationship was determined in Triton-X skinned muscle bundles activated at pCa 9.0 to 4.0. In strips maximally activated at pCa 4.0, the following parameters of cross-bridge cycling were measured: (1) rate of force redevelopment following rapid shortening and restretching (k(tr)); and (2) isometric stiffness at maximal activation and in rigor. The fraction of attached cross-bridges (α(fs)) and apparent rate constants for cross-bridge attachment (f(app)) and detachment (g(app)) were derived assuming a two-state model for cross-bridge cycling. Compared to the adult, the force-pCa curve for neonatal cardiac muscle was significantly shifted to the left. Neonatal cardiac muscle also displayed significantly smaller α(fs), slower k(tr) and f(app); however, g(app) was not significantly different between age groups. These data indicate that weaker force production in neonatal cardiac muscle involves, at least in part, less efficient cross-bridge cycling kinetics.
AB - The developmental shift in contractile protein isoform expression in the rodent heart likely affects actin-myosin cross-bridge interactions. We compared the Ca2+ sensitivity for force generation and cross-bridge cycling kinetics in neonatal (postnatal days 0-3) and adult (day 84) rats. The force-pCa relationship was determined in Triton-X skinned muscle bundles activated at pCa 9.0 to 4.0. In strips maximally activated at pCa 4.0, the following parameters of cross-bridge cycling were measured: (1) rate of force redevelopment following rapid shortening and restretching (k(tr)); and (2) isometric stiffness at maximal activation and in rigor. The fraction of attached cross-bridges (α(fs)) and apparent rate constants for cross-bridge attachment (f(app)) and detachment (g(app)) were derived assuming a two-state model for cross-bridge cycling. Compared to the adult, the force-pCa curve for neonatal cardiac muscle was significantly shifted to the left. Neonatal cardiac muscle also displayed significantly smaller α(fs), slower k(tr) and f(app); however, g(app) was not significantly different between age groups. These data indicate that weaker force production in neonatal cardiac muscle involves, at least in part, less efficient cross-bridge cycling kinetics.
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U2 - 10.1023/A:1005585807179
DO - 10.1023/A:1005585807179
M3 - Article
C2 - 10672520
AN - SCOPUS:0033374550
SN - 0142-4319
VL - 20
SP - 717
EP - 723
JO - Journal of Muscle Research and Cell Motility
JF - Journal of Muscle Research and Cell Motility
IS - 7
ER -