@article{3114ca93134f4f5e806f53bbbb1e1a79,
title = "Comparative Oncogenomics Identifies NEDD9 as a Melanoma Metastasis Gene",
abstract = "Genomes of human cancer cells are characterized by numerous chromosomal aberrations of uncertain pathogenetic significance. Here, in an inducible mouse model of melanoma, we characterized metastatic variants with an acquired focal chromosomal amplification that corresponds to a much larger amplification in human metastatic melanomas. Further analyses identified Nedd9, an adaptor protein related to p130CAS, as the only gene within the minimal common region that exhibited amplification-associated overexpression. A series of functional, biochemical, and clinical studies established NEDD9 as a bona fide melanoma metastasis gene. NEDD9 enhanced invasion in vitro and metastasis in vivo of both normal and transformed melanocytes, functionally interacted with focal adhesion kinase and modulated focal contact formation, and exhibited frequent robust overexpression in human metastatic melanoma relative to primary melanoma. Thus, comparative oncogenomics has enabled the identification and facilitated the validation of a highly relevant cancer gene governing metastatic potential in human melanoma.",
author = "Minjung Kim and Gans, {Joseph D.} and Cristina Nogueira and Audrey Wang and Paik, {Ji Hye} and Bin Feng and Cameron Brennan and Hahn, {William C.} and Carlos Cordon-Cardo and Wagner, {Stephan N.} and Flotte, {Thomas J.} and Duncan, {Lyn M.} and Granter, {Scott R.} and Lynda Chin",
note = "Funding Information: We thank Dr. Ron DePinho for critical review of the manuscript and members of the Chin and DePinho laboratories for helpful discussion. Genome-wide array-CGH profiles were generated at the Arthur and Rochelle Belfer Cancer Genomics Center at DFCI, and the high-density mouse tiling array-CGH profiles were generated by Drs. Rebecca Selzer and Todd Richmond of NimbleGen. We are also grateful to Shan Jiang, Karen Marmon, Alice Yu, and Yan Zhang for their assistance in the animal facility. We would also like to thank the Harvard-UPenn-MD Anderson Skin SPORE programs for the Melanoma Progression TMA and the RNAi Consortium (TRC) at the Broad Institute for lentiviral shRNA vectors and Sara Bulmer for technical assistance with RNAi distribution. W.C.H. is supported by U01 CA105423 and P50 CA112962. S.N.W. is supported by the Austrian National Bank (No. 11062). J.-H.P. is a Damon Runyon Fellow. This work was supported by R01 CA93947, U01 CA84313, and P50 CA93683 to L.C. ",
year = "2006",
month = jun,
day = "30",
doi = "10.1016/j.cell.2006.06.008",
language = "English (US)",
volume = "125",
pages = "1269--1281",
journal = "Cell",
issn = "0092-8674",
publisher = "Cell Press",
number = "7",
}