Comparative genomic hybridization: A comparison with molecular and cytogenetic analysis

Elisabeth P. Nacheva, Colin D. Grace, Michael Bittner, David H. Ledbetter, Robert B. Jenkins, Anthony R. Green

Research output: Contribution to journalArticle

25 Scopus citations

Abstract

Comparative genomic hybridization (CGH) is A powerful technique/or detecting copy number changes throughout the genome. We describe the development of a versatile image analysis program for CGH studies. Several methods for the production of metaphasee which give optimum hybridization signals have also been assessed. CGH analysis was performed on DNA samples from several different and clinically relevant specimens: amniotic fluid cells trisomic for a single chromosome, lymphoblastoid cell lines with abnormalities involving single chromosome bands, malignant cell lines and biopsy material from primary ovarian carcinomas. The results were compared with those derived from G-banding, chromosome painting, and molecular genetic techniques. Our data demonstrate that CGH was able to detect a wide range of quantitative genetic alterations including duplication or deletion of single chromosome bands. CGH analysis also indicated the presence of genetic abnormalities that were not detected by other cytogenetic or molecular approaches. Moreover, our CGH methodology allowed the ready comparison of CGH results from different tumors, a process which greatly facilitated identification of shared genetic changes.

Original languageEnglish (US)
Pages (from-to)93-105
Number of pages13
JournalCancer Genetics and Cytogenetics
Volume100
Issue number2
DOIs
StatePublished - Jan 15 1998

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ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

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