Abstract
Comparative genomic hybridization (CGH) is A powerful technique/or detecting copy number changes throughout the genome. We describe the development of a versatile image analysis program for CGH studies. Several methods for the production of metaphasee which give optimum hybridization signals have also been assessed. CGH analysis was performed on DNA samples from several different and clinically relevant specimens: amniotic fluid cells trisomic for a single chromosome, lymphoblastoid cell lines with abnormalities involving single chromosome bands, malignant cell lines and biopsy material from primary ovarian carcinomas. The results were compared with those derived from G-banding, chromosome painting, and molecular genetic techniques. Our data demonstrate that CGH was able to detect a wide range of quantitative genetic alterations including duplication or deletion of single chromosome bands. CGH analysis also indicated the presence of genetic abnormalities that were not detected by other cytogenetic or molecular approaches. Moreover, our CGH methodology allowed the ready comparison of CGH results from different tumors, a process which greatly facilitated identification of shared genetic changes.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 93-105 |
| Number of pages | 13 |
| Journal | Cancer Genetics and Cytogenetics |
| Volume | 100 |
| Issue number | 2 |
| DOIs | |
| State | Published - Jan 15 1998 |
Fingerprint
ASJC Scopus subject areas
- Cancer Research
- Genetics
- Molecular Biology
Cite this
Comparative genomic hybridization : A comparison with molecular and cytogenetic analysis. / Nacheva, Elisabeth P.; Grace, Colin D.; Bittner, Michael; Ledbetter, David H.; Jenkins, Robert Brian; Green, Anthony R.
In: Cancer Genetics and Cytogenetics, Vol. 100, No. 2, 15.01.1998, p. 93-105.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Comparative genomic hybridization
T2 - A comparison with molecular and cytogenetic analysis
AU - Nacheva, Elisabeth P.
AU - Grace, Colin D.
AU - Bittner, Michael
AU - Ledbetter, David H.
AU - Jenkins, Robert Brian
AU - Green, Anthony R.
PY - 1998/1/15
Y1 - 1998/1/15
N2 - Comparative genomic hybridization (CGH) is A powerful technique/or detecting copy number changes throughout the genome. We describe the development of a versatile image analysis program for CGH studies. Several methods for the production of metaphasee which give optimum hybridization signals have also been assessed. CGH analysis was performed on DNA samples from several different and clinically relevant specimens: amniotic fluid cells trisomic for a single chromosome, lymphoblastoid cell lines with abnormalities involving single chromosome bands, malignant cell lines and biopsy material from primary ovarian carcinomas. The results were compared with those derived from G-banding, chromosome painting, and molecular genetic techniques. Our data demonstrate that CGH was able to detect a wide range of quantitative genetic alterations including duplication or deletion of single chromosome bands. CGH analysis also indicated the presence of genetic abnormalities that were not detected by other cytogenetic or molecular approaches. Moreover, our CGH methodology allowed the ready comparison of CGH results from different tumors, a process which greatly facilitated identification of shared genetic changes.
AB - Comparative genomic hybridization (CGH) is A powerful technique/or detecting copy number changes throughout the genome. We describe the development of a versatile image analysis program for CGH studies. Several methods for the production of metaphasee which give optimum hybridization signals have also been assessed. CGH analysis was performed on DNA samples from several different and clinically relevant specimens: amniotic fluid cells trisomic for a single chromosome, lymphoblastoid cell lines with abnormalities involving single chromosome bands, malignant cell lines and biopsy material from primary ovarian carcinomas. The results were compared with those derived from G-banding, chromosome painting, and molecular genetic techniques. Our data demonstrate that CGH was able to detect a wide range of quantitative genetic alterations including duplication or deletion of single chromosome bands. CGH analysis also indicated the presence of genetic abnormalities that were not detected by other cytogenetic or molecular approaches. Moreover, our CGH methodology allowed the ready comparison of CGH results from different tumors, a process which greatly facilitated identification of shared genetic changes.
UR - http://www.scopus.com/inward/record.url?scp=0344382056&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0344382056&partnerID=8YFLogxK
U2 - 10.1016/S0165-4608(97)00021-6
DO - 10.1016/S0165-4608(97)00021-6
M3 - Article
C2 - 9428351
AN - SCOPUS:0344382056
VL - 100
SP - 93
EP - 105
JO - Cancer Genetics and Cytogenetics
JF - Cancer Genetics and Cytogenetics
SN - 0165-4608
IS - 2
ER -