Comparative dynamics of microglial and glioma cell motility at the infiltrative margin of brain tumours

Joseph Juliano, Orlando Gil, Andrea Hawkins-Daarud, Sonal Noticewala, Russell C. Rockne, Jill Gallaher, Susan Christine Massey, Peter A. Sims, Alexander R.A. Anderson, Kristin R. Swanson, Peter Canoll

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Microglia are a major cellular component of gliomas, and abundant in the centre of the tumour and at the infiltrative margins. While glioma is a notoriously infiltrative disease, the dynamics of microglia and glioma migratory patterns have not been well characterized. To investigate the migratory behaviour of microglia and glioma cells at the infiltrative edge, we performed two-colour time-lapse fluorescence microscopy of brain slices generated from a platelet-derived growth factor-B (PDGFB)-driven rat model of glioma, in which glioma cells and microglia were each labelled with one of two different fluorescent markers. We used mathematical techniques to analyse glioma cells and microglia motility with both single cell tracking and particle image velocimetry (PIV). Our results show microglia motility is strongly correlated with the presence of glioma, while the correlation of the speeds of glioma cells and microglia was variable and weak. Additionally, we showed that microglia and glioma cells exhibit different types of diffusive migratory behaviour. Microglia movement fit a simple random walk, while glioma cell movement fits a super diffusion pattern. These results show that glioma cells stimulate microglia motility at the infiltrative margins, creating a correlation between the spatial distribution of glioma cells and the pattern of microglia motility.

Original languageEnglish (US)
Article number20170582.
JournalJournal of the Royal Society Interface
Volume15
Issue number139
DOIs
StatePublished - Jan 1 2018

Keywords

  • Anomalous diffusion
  • Glioblastoma
  • PDGF rat model
  • Particle image velocimetry
  • Time lapse microscopy

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Bioengineering
  • Biomaterials
  • Biochemistry
  • Biomedical Engineering

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