TY - JOUR
T1 - Comparative dynamics of microglial and glioma cell motility at the infiltrative margin of brain tumours
AU - Juliano, Joseph
AU - Gil, Orlando
AU - Hawkins-Daarud, Andrea
AU - Noticewala, Sonal
AU - Rockne, Russell C.
AU - Gallaher, Jill
AU - Massey, Susan Christine
AU - Sims, Peter A.
AU - Anderson, Alexander R.A.
AU - Swanson, Kristin R.
AU - Canoll, Peter
N1 - Publisher Copyright:
© 2018 The Author(s) Published by the Royal Society. All rights reserved.
PY - 2018
Y1 - 2018
N2 - Microglia are a major cellular component of gliomas, and abundant in the centre of the tumour and at the infiltrative margins. While glioma is a notoriously infiltrative disease, the dynamics of microglia and glioma migratory patterns have not been well characterized. To investigate the migratory behaviour of microglia and glioma cells at the infiltrative edge, we performed two-colour time-lapse fluorescence microscopy of brain slices generated from a platelet-derived growth factor-B (PDGFB)-driven rat model of glioma, in which glioma cells and microglia were each labelled with one of two different fluorescent markers. We used mathematical techniques to analyse glioma cells and microglia motility with both single cell tracking and particle image velocimetry (PIV). Our results show microglia motility is strongly correlated with the presence of glioma, while the correlation of the speeds of glioma cells and microglia was variable and weak. Additionally, we showed that microglia and glioma cells exhibit different types of diffusive migratory behaviour. Microglia movement fit a simple random walk, while glioma cell movement fits a super diffusion pattern. These results show that glioma cells stimulate microglia motility at the infiltrative margins, creating a correlation between the spatial distribution of glioma cells and the pattern of microglia motility.
AB - Microglia are a major cellular component of gliomas, and abundant in the centre of the tumour and at the infiltrative margins. While glioma is a notoriously infiltrative disease, the dynamics of microglia and glioma migratory patterns have not been well characterized. To investigate the migratory behaviour of microglia and glioma cells at the infiltrative edge, we performed two-colour time-lapse fluorescence microscopy of brain slices generated from a platelet-derived growth factor-B (PDGFB)-driven rat model of glioma, in which glioma cells and microglia were each labelled with one of two different fluorescent markers. We used mathematical techniques to analyse glioma cells and microglia motility with both single cell tracking and particle image velocimetry (PIV). Our results show microglia motility is strongly correlated with the presence of glioma, while the correlation of the speeds of glioma cells and microglia was variable and weak. Additionally, we showed that microglia and glioma cells exhibit different types of diffusive migratory behaviour. Microglia movement fit a simple random walk, while glioma cell movement fits a super diffusion pattern. These results show that glioma cells stimulate microglia motility at the infiltrative margins, creating a correlation between the spatial distribution of glioma cells and the pattern of microglia motility.
KW - Anomalous diffusion
KW - Glioblastoma
KW - PDGF rat model
KW - Particle image velocimetry
KW - Time lapse microscopy
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U2 - 10.1098/rsif.2017.0582
DO - 10.1098/rsif.2017.0582
M3 - Article
C2 - 29445035
AN - SCOPUS:85048518884
SN - 1742-5689
VL - 15
JO - Journal of the Royal Society Interface
JF - Journal of the Royal Society Interface
IS - 139
M1 - 20170582.
ER -