Communication of agonist-induced electrical responses along 'capillaries' in vitro can be modulated by lipopolysaccharide, but not nitric oxide

Darcy Lidington, Yves Ouellette, Karel Tyml

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Communication of agonist-induced membrane potential changes along blood vessels has been proposed to contribute to the coordination of microvascular function. Factors mediating septic shock may compromise this coordination. Using electrophysiology in a simplified in vitro model of endothelial cells grown as capillary-like structures, we aimed to determine (i) the effect of lipopolysaccharide (LPS) on endothelial cell membrane potential responses to ATP and KCl and (ii) the effect of LPS and nitric oxide (NO) on cell-to-cell communication. Treatment of 'capillaries' with LPS (10 μg/ml for 1 h) did not affect local responsiveness to ATP or KCl, but reduced cell communication by a tyrosine-kinase-dependent mechanism. Treatment of 'capillaries' with the NO donor DETA (100 μM) or the NO synthase inhibitor L-NAME (100 μM) had no effect on cell communication or the response to LPS. Endogenous NO production, stimulated by LPS + interferon-γ (100 U/ml) treatment, also had no effect on cell communication beyond that of LPS alone. We conclude that LPS, but not NO, can modulate conduction of agonist-induced electrical responses along endothelial capillary-like structures in vitro.

Original languageEnglish (US)
Pages (from-to)405-413
Number of pages9
JournalJournal of Vascular Research
Volume39
Issue number5
DOIs
StatePublished - Oct 17 2002
Externally publishedYes

Fingerprint

Lipopolysaccharides
Nitric Oxide
Cell Communication
Membrane Potentials
Endothelial Cells
Adenosine Triphosphate
DEET
Nitric Oxide Donors
Electrophysiology
NG-Nitroarginine Methyl Ester
Septic Shock
In Vitro Techniques
Nitric Oxide Synthase
Protein-Tyrosine Kinases
Interferons
Blood Vessels
Cell Membrane

Keywords

  • Cell culture
  • Electrophysiology
  • Endothelial cells
  • Gap junctions
  • Lipopolysaccharide

ASJC Scopus subject areas

  • Physiology

Cite this

Communication of agonist-induced electrical responses along 'capillaries' in vitro can be modulated by lipopolysaccharide, but not nitric oxide. / Lidington, Darcy; Ouellette, Yves; Tyml, Karel.

In: Journal of Vascular Research, Vol. 39, No. 5, 17.10.2002, p. 405-413.

Research output: Contribution to journalArticle

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