Communication of agonist-induced electrical responses along 'capillaries' in vitro can be modulated by lipopolysaccharide, but not nitric oxide

Darcy Lidington, Yves Ouellette, Karel Tyml

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

Communication of agonist-induced membrane potential changes along blood vessels has been proposed to contribute to the coordination of microvascular function. Factors mediating septic shock may compromise this coordination. Using electrophysiology in a simplified in vitro model of endothelial cells grown as capillary-like structures, we aimed to determine (i) the effect of lipopolysaccharide (LPS) on endothelial cell membrane potential responses to ATP and KCl and (ii) the effect of LPS and nitric oxide (NO) on cell-to-cell communication. Treatment of 'capillaries' with LPS (10 μg/ml for 1 h) did not affect local responsiveness to ATP or KCl, but reduced cell communication by a tyrosine-kinase-dependent mechanism. Treatment of 'capillaries' with the NO donor DETA (100 μM) or the NO synthase inhibitor L-NAME (100 μM) had no effect on cell communication or the response to LPS. Endogenous NO production, stimulated by LPS + interferon-γ (100 U/ml) treatment, also had no effect on cell communication beyond that of LPS alone. We conclude that LPS, but not NO, can modulate conduction of agonist-induced electrical responses along endothelial capillary-like structures in vitro.

Original languageEnglish (US)
Pages (from-to)405-413
Number of pages9
JournalJournal of Vascular Research
Volume39
Issue number5
DOIs
StatePublished - Oct 17 2002

Keywords

  • Cell culture
  • Electrophysiology
  • Endothelial cells
  • Gap junctions
  • Lipopolysaccharide

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

Fingerprint Dive into the research topics of 'Communication of agonist-induced electrical responses along 'capillaries' in vitro can be modulated by lipopolysaccharide, but not nitric oxide'. Together they form a unique fingerprint.

  • Cite this