Common genetic variation and haplotypes of the anion exchanger SLC4A2 in primary biliary cirrhosis

Brian D. Juran, Elizabeth J. Atkinson, Joseph J. Larson, Erik M. Schlicht, Konstantinos N. Lazaridis

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Abstract

OBJECTIVES:Deficiencies of the anion exchanger SLC4A2 are thought to play a pathogenic role in primary biliary cirrhosis (PBC), as the evidenced by decreased expression and activity in PBC patients and development of disease features in SLC4A2 knockout mice. We hypothesized that genetic variation in SLC4A2 might influence this pathogenic contribution. Thus, we aimed to perform a comprehensive assessment of SLC4A2 genetic variation in PBC using a linkage disequilibrium (LD)-based haplotype-tagging approach.METHODS:Twelve single nucleotide polymorphisms (SNPs) across SLC4A2 were genotyped in 409 PBC patients and 300 controls and evaluated for association with disease, as well as with prior orthotopic liver transplant and antimitochondrial antibody (AMA) status among the PBC patients, both individually and as inferred haplotypes, using logistic regression.RESULTS:All SNPs were in Hardy-Weinberg equilibrium. No associations with disease or liver transplantation were detected, but two variants, rs2303929 and rs3793336, were associated with negativity for antimitochondrial antibodies among the PBC patients.CONCLUSIONS:The common genetic variation of SLC4A2 does not directly affect the risk of PBC or its clinical outcome. Whether the deficiency of SLC4A2 expression and activity observed earlier in PBC patients is an acquired epiphenomenon of underlying disease or is because of heritable factors in unappreciated regulatory regions remains uncertain. Of note, two SLC4A2 variants appear to influence AMA status among PBC patients. The mechanisms behind this finding are unclear.

Original languageEnglish (US)
Pages (from-to)1406-1411
Number of pages6
JournalAmerican Journal of Gastroenterology
Volume104
Issue number6
DOIs
StatePublished - Jun 1 2009

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ASJC Scopus subject areas

  • Hepatology
  • Gastroenterology

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