Commentary on Maspin modulates prostate cancer cell apoptotic and angiogenic response to hypoxia via targeting AKT. McKenzie S, Sakamoto S, Kyprianou N, Division of Urology, Department of Surgery, Markey Cancer Center, University of Kentucky College of Medicine, Lexington, KY

Research output: Contribution to journalArticle

Abstract

Hypoxia has been previously linked to the development of both benign prostatic hyperplasia and prostate cancer. This study investigated the effect of maspin, an extracellular matrix (ECM) tumor suppressor, on the apoptotic response of prostate cancer cells to hypoxia. Gene expression profiling of human benign and malignant prostate epithelial cells after exposure to hypoxia or normoxia revealed dramatic changes in ECM regulators. Maspin was found to be overexpressed in response to hypoxia in prostate cancer cells, but not in benign prostate cells. To dissect the contribution of maspin to tumor cell responses within a hypoxic microenvironment, we used maspin-overexpressing DU-145 human prostate cancer cells. Exposure to hypoxic conditions (1% O (2)) led to a significant increase in apoptosis in the DU-145 maspin cells, compared with DU-145 neotransfectants without a significant effect on cell migration. This enhanced sensitivity to hypoxia-induced apoptosis leads to a significant suppression of tumor growth and tumor vascularity in vivo by targeting Akt and focal adhesion kinase activation. Our findings implicate maspin in prostate cancer cell response to hypoxia via recruitment of intracellular signaling partners. This study may have significance in the identification of maspin-driven therapeutic targeting in advanced metastatic prostate cancer.

Original languageEnglish (US)
Pages (from-to)231
Number of pages1
JournalUrologic Oncology: Seminars and Original Investigations
Volume27
Issue number2
DOIs
StatePublished - Mar 2009
Externally publishedYes

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Urology
Prostatic Neoplasms
Medicine
Neoplasms
Extracellular Matrix
Prostate
Apoptosis
Cell Hypoxia
Focal Adhesion Protein-Tyrosine Kinases
Prostatic Hyperplasia
Gene Expression Profiling
Hypoxia
SERPIN-B5
Cell Movement
Epithelial Cells
Growth

ASJC Scopus subject areas

  • Oncology
  • Urology

Cite this

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abstract = "Hypoxia has been previously linked to the development of both benign prostatic hyperplasia and prostate cancer. This study investigated the effect of maspin, an extracellular matrix (ECM) tumor suppressor, on the apoptotic response of prostate cancer cells to hypoxia. Gene expression profiling of human benign and malignant prostate epithelial cells after exposure to hypoxia or normoxia revealed dramatic changes in ECM regulators. Maspin was found to be overexpressed in response to hypoxia in prostate cancer cells, but not in benign prostate cells. To dissect the contribution of maspin to tumor cell responses within a hypoxic microenvironment, we used maspin-overexpressing DU-145 human prostate cancer cells. Exposure to hypoxic conditions (1{\%} O (2)) led to a significant increase in apoptosis in the DU-145 maspin cells, compared with DU-145 neotransfectants without a significant effect on cell migration. This enhanced sensitivity to hypoxia-induced apoptosis leads to a significant suppression of tumor growth and tumor vascularity in vivo by targeting Akt and focal adhesion kinase activation. Our findings implicate maspin in prostate cancer cell response to hypoxia via recruitment of intracellular signaling partners. This study may have significance in the identification of maspin-driven therapeutic targeting in advanced metastatic prostate cancer.",
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