Commentary: Comparison of current flow cytometry methods for monoclonal B cell lymphocytosis detection

Wendy G. Nieto, Julia Almeida, Cristina Teodosio, Fatima Abbasi, Sallie D. Allgood, Fiona Connors, Jane M. Rachel, Paolo Ghia, Mark C. Lanasa, Andy C. Rawstron, Alberto Orfao, Neil E. Caporaso, Curt A. Hanson, Youn K. Shim, Robert F. Vogt, Gerald E. Marti

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Monoclonal B cell lymphocytosis (MBL) is now recognized as the B-lymphocyte analogue of a monoclonal gammopathy of unknown significance. MBL can be the precursor of chronic lymphocytic leukemia or associated with non-Hodgkin's lymphoma. It may be associated with an autoimmune abnormality or be related to aging (immunosenescence). The combination of available new fluorochrome- conjugated monoclonal antibody reagents, multilaser instrumentation, and improved software tools have led to a new level of multicolor analysis of MBL. Presently, several centers, including the University of Salamanca (Spain), Duke University (Durham, NC), Mayo Clinic (Rochester, MN), and the National Cancer Institute (Bethesda, MD) in conjunction with the Genetics and Epidemiology of Familial chronic lymphocytic leukemia Consortium, the Food and Drug Administration (Bethesda, MD), and the Centers for Disease Control and Prevention/Agency for Toxic Substances and Disease Registry (Atlanta, GA) in collaboration with Saint Luke's Hospital (Kansas City, MO), the Università Vita-Salute San Raffaele in Milan (Italy), and Leeds Teaching Hospital (UK) are all actively conducting studies on MBL. This commentary is an updated summary of the current methods used in these centers. It is important to note the diversity of use in reagents, instruments, and methods of analysis. Despite this diversity, there is a consensus in what constitutes the diagnosis of MBL and its subtypes. There is also an emerging consensus on what the next investigative steps should be.

Original languageEnglish (US)
JournalCytometry Part B - Clinical Cytometry
Volume78
Issue numberSUPPL. 1
DOIs
StatePublished - 2010

Fingerprint

Lymphocytosis
Flow Cytometry
B-Lymphocytes
B-Cell Chronic Lymphocytic Leukemia
Paraproteinemias
Molecular Epidemiology
National Cancer Institute (U.S.)
Poisons
Urban Hospitals
United States Food and Drug Administration
Centers for Disease Control and Prevention (U.S.)
Fluorescent Dyes
Teaching Hospitals
Spain
Non-Hodgkin's Lymphoma
Italy
Registries
Software
Monoclonal Antibodies

Keywords

  • Chronic lymphocytic leukemia
  • Immunophenotyping
  • Lymphocyte gating

ASJC Scopus subject areas

  • Cell Biology
  • Histology
  • Pathology and Forensic Medicine

Cite this

Nieto, W. G., Almeida, J., Teodosio, C., Abbasi, F., Allgood, S. D., Connors, F., ... Marti, G. E. (2010). Commentary: Comparison of current flow cytometry methods for monoclonal B cell lymphocytosis detection. Cytometry Part B - Clinical Cytometry, 78(SUPPL. 1). https://doi.org/10.1002/cyto.b.20556

Commentary : Comparison of current flow cytometry methods for monoclonal B cell lymphocytosis detection. / Nieto, Wendy G.; Almeida, Julia; Teodosio, Cristina; Abbasi, Fatima; Allgood, Sallie D.; Connors, Fiona; Rachel, Jane M.; Ghia, Paolo; Lanasa, Mark C.; Rawstron, Andy C.; Orfao, Alberto; Caporaso, Neil E.; Hanson, Curt A.; Shim, Youn K.; Vogt, Robert F.; Marti, Gerald E.

In: Cytometry Part B - Clinical Cytometry, Vol. 78, No. SUPPL. 1, 2010.

Research output: Contribution to journalArticle

Nieto, WG, Almeida, J, Teodosio, C, Abbasi, F, Allgood, SD, Connors, F, Rachel, JM, Ghia, P, Lanasa, MC, Rawstron, AC, Orfao, A, Caporaso, NE, Hanson, CA, Shim, YK, Vogt, RF & Marti, GE 2010, 'Commentary: Comparison of current flow cytometry methods for monoclonal B cell lymphocytosis detection', Cytometry Part B - Clinical Cytometry, vol. 78, no. SUPPL. 1. https://doi.org/10.1002/cyto.b.20556
Nieto, Wendy G. ; Almeida, Julia ; Teodosio, Cristina ; Abbasi, Fatima ; Allgood, Sallie D. ; Connors, Fiona ; Rachel, Jane M. ; Ghia, Paolo ; Lanasa, Mark C. ; Rawstron, Andy C. ; Orfao, Alberto ; Caporaso, Neil E. ; Hanson, Curt A. ; Shim, Youn K. ; Vogt, Robert F. ; Marti, Gerald E. / Commentary : Comparison of current flow cytometry methods for monoclonal B cell lymphocytosis detection. In: Cytometry Part B - Clinical Cytometry. 2010 ; Vol. 78, No. SUPPL. 1.
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