Combined I-124 positron emission tomography/computed tomography imaging of NIS gene expression in animal models of stably transfected and intravenously transfected tumor

David M Dingli, Brad J. Kemp, Michael K. O'Connor, John C. Morris, Stephen J Russell, Val Lowe

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Purpose: With the advent of replication competent viruses for cancer gene therapy, it has become imperative to monitor the biodistribution, expression and replication of these vectors in living organisms. We evaluated the potential of I-124 positron emission tomography (PET)/ computed tomography (CT) imaging in gene therapy animal models utilizing the sodium iodide symporter (NIS) and compared the findings to I-123 gamma camera imaging. Procedures: CB17 SCID mice were implanted with myeloma cell lines expressing NIS or infected by MV-NIS given systemically. Mice were imaged by both gamma camera (I-123) and PET/CT (I-124) and image quality assessed. Results: NIS expressing tumors concentrated 7.1% of the injected activity while tumors infected with the control virus had only 0.3% of the activity injected. Conclusions: I-124 PET/CT in combination with NIS allows the tracking of stably transfected tumors or intravenously transfected tumors. Combined modality imaging using PET/CT allows accurate and non-invasive imaging of the distribution and gene expression of a replicating viral vector in living systems.

Original languageEnglish (US)
Pages (from-to)16-23
Number of pages8
JournalMolecular Imaging and Biology
Volume8
Issue number1
DOIs
StatePublished - Jan 2006

Fingerprint

Animal Models
Gene Expression
Genetic Therapy
Neoplasms
Gamma Cameras
SCID Mice
Neoplasm Genes
Virus Replication
Radionuclide Imaging
Viruses
Cell Line
Positron Emission Tomography Computed Tomography

Keywords

  • Computerized tomography
  • Gamma camera
  • Isotopes
  • Measles virus
  • Molecular imaging
  • Oncolysis
  • Positron emission tomography
  • Sodium iodide symporter
  • Virotherapy

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Biology
  • Radiology Nuclear Medicine and imaging

Cite this

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title = "Combined I-124 positron emission tomography/computed tomography imaging of NIS gene expression in animal models of stably transfected and intravenously transfected tumor",
abstract = "Purpose: With the advent of replication competent viruses for cancer gene therapy, it has become imperative to monitor the biodistribution, expression and replication of these vectors in living organisms. We evaluated the potential of I-124 positron emission tomography (PET)/ computed tomography (CT) imaging in gene therapy animal models utilizing the sodium iodide symporter (NIS) and compared the findings to I-123 gamma camera imaging. Procedures: CB17 SCID mice were implanted with myeloma cell lines expressing NIS or infected by MV-NIS given systemically. Mice were imaged by both gamma camera (I-123) and PET/CT (I-124) and image quality assessed. Results: NIS expressing tumors concentrated 7.1{\%} of the injected activity while tumors infected with the control virus had only 0.3{\%} of the activity injected. Conclusions: I-124 PET/CT in combination with NIS allows the tracking of stably transfected tumors or intravenously transfected tumors. Combined modality imaging using PET/CT allows accurate and non-invasive imaging of the distribution and gene expression of a replicating viral vector in living systems.",
keywords = "Computerized tomography, Gamma camera, Isotopes, Measles virus, Molecular imaging, Oncolysis, Positron emission tomography, Sodium iodide symporter, Virotherapy",
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AU - Dingli, David M

AU - Kemp, Brad J.

AU - O'Connor, Michael K.

AU - Morris, John C.

AU - Russell, Stephen J

AU - Lowe, Val

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N2 - Purpose: With the advent of replication competent viruses for cancer gene therapy, it has become imperative to monitor the biodistribution, expression and replication of these vectors in living organisms. We evaluated the potential of I-124 positron emission tomography (PET)/ computed tomography (CT) imaging in gene therapy animal models utilizing the sodium iodide symporter (NIS) and compared the findings to I-123 gamma camera imaging. Procedures: CB17 SCID mice were implanted with myeloma cell lines expressing NIS or infected by MV-NIS given systemically. Mice were imaged by both gamma camera (I-123) and PET/CT (I-124) and image quality assessed. Results: NIS expressing tumors concentrated 7.1% of the injected activity while tumors infected with the control virus had only 0.3% of the activity injected. Conclusions: I-124 PET/CT in combination with NIS allows the tracking of stably transfected tumors or intravenously transfected tumors. Combined modality imaging using PET/CT allows accurate and non-invasive imaging of the distribution and gene expression of a replicating viral vector in living systems.

AB - Purpose: With the advent of replication competent viruses for cancer gene therapy, it has become imperative to monitor the biodistribution, expression and replication of these vectors in living organisms. We evaluated the potential of I-124 positron emission tomography (PET)/ computed tomography (CT) imaging in gene therapy animal models utilizing the sodium iodide symporter (NIS) and compared the findings to I-123 gamma camera imaging. Procedures: CB17 SCID mice were implanted with myeloma cell lines expressing NIS or infected by MV-NIS given systemically. Mice were imaged by both gamma camera (I-123) and PET/CT (I-124) and image quality assessed. Results: NIS expressing tumors concentrated 7.1% of the injected activity while tumors infected with the control virus had only 0.3% of the activity injected. Conclusions: I-124 PET/CT in combination with NIS allows the tracking of stably transfected tumors or intravenously transfected tumors. Combined modality imaging using PET/CT allows accurate and non-invasive imaging of the distribution and gene expression of a replicating viral vector in living systems.

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