Coculture with autologous myotubes of cytotoxic T cells isolated from muscle in inflammatory myopathies

R. Hohlfeld, Andrew G Engel

Research output: Contribution to journalArticle

82 Citations (Scopus)

Abstract

T-cell lines were expanded from muscle of 10 patients with polymyositis, 5 with inclusion body myositis, 5 with dermatomyositis, and 5 with other muscle diseases. All cell lines uniformly expressed T-cell antigens, but not natural killer cell or B-cell antigens. The proportion of helper (CD4+) and cytotoxic (CD8+) T cells in the expanded lines was variable and showed no correlation with the diagnosis. Sixteen cell lines (6 polymyositis, 4 inclusion body myositis, 5 dermatomyositis, 1 other muscle disease) consisted predominantly of CD8+ T cells. None of these lines displayed natural killer-like cytotoxicity but all were capable of lectin-dependent cytotoxicity. Three of 6 polymyositis, 1 of 4 inclusion body myositis, and 1 of 5 dermatomyositis lines showed low but statistically significant cytotoxicity against autologous myotubes (6 to 27% specific 51Cr release; effector-target ratio, 20:1). The results demonstrate that functionally competent cytotoxic T cells can be expanded from muscle affected by inflammatory myopathies and are consistent with the hypothesis that some cytotoxic T cells recognize an autoantigen on myotubes. Further studies of this experimental system may define the molecular mechanism of T cell-mediated muscle fiber injury and may help to identify the relevant antigens.

Original languageEnglish (US)
Pages (from-to)498-507
Number of pages10
JournalAnnals of Neurology
Volume29
Issue number5
StatePublished - 1991

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Myositis
Skeletal Muscle Fibers
Coculture Techniques
T-Lymphocytes
Muscles
Inclusion Body Myositis
Polymyositis
Dermatomyositis
Cell Line
Antigens
Viral Tumor Antigens
Autoantigens
Lectins
Natural Killer Cells
B-Lymphocytes
Wounds and Injuries

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Coculture with autologous myotubes of cytotoxic T cells isolated from muscle in inflammatory myopathies. / Hohlfeld, R.; Engel, Andrew G.

In: Annals of Neurology, Vol. 29, No. 5, 1991, p. 498-507.

Research output: Contribution to journalArticle

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