Cloning of the murine eosinophil peroxidase gene (mEPO): Characterization of a conserved subgroup of mammalian hematopoietic peroxidases

Margaret A. Horton, Kirsten A. Larson, James J. Lee, Nancy A. Lee

Research output: Contribution to journalArticle

51 Scopus citations

Abstract

The mouse eosinophil peroxidase (mEPO) gene was cloned by screening a randomprimed bone marrow cDNA Library at reduced criteria using a hEPO cDNA. An mEPO cDNA was subsequently used to isolate the mEPO gene from a λ-genomic library. The mEPO gene displays a high degree of conservation with its human homologue: the transcription units are approximately the same size, conserve the relative size and position of the 12 exons associated with each gene, and at a nucleotide level the mouse and human EPO genes are 86% identical in the protein coding regions and 66% identical in the 3'-untranslated trailer regions. This strong conservation extends to the encoded proteins which show ~90% amino acid identity. Expression of the mEPO gene is restricted to tissues containing eosinophil progenitor cells (e.g., bone marrow and spleen), a pattern similar to the expression of another murine eosinophil granule protein, major basic protein.

Original languageEnglish (US)
Pages (from-to)285-294
Number of pages10
JournalJournal of Leukocyte Biology
Volume60
Issue number2
DOIs
StatePublished - Aug 1996

Keywords

  • Evolution
  • Gene organization
  • Granule proteins
  • Mouse eosinophils

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Cell Biology

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