Cloning of the murine eosinophil peroxidase gene (mEPO): Characterization of a conserved subgroup of mammalian hematopoietic peroxidases

Margaret A. Horton, Kirsten A. Larson, James J. Lee, Nancy A Lee

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

The mouse eosinophil peroxidase (mEPO) gene was cloned by screening a randomprimed bone marrow cDNA Library at reduced criteria using a hEPO cDNA. An mEPO cDNA was subsequently used to isolate the mEPO gene from a λ-genomic library. The mEPO gene displays a high degree of conservation with its human homologue: the transcription units are approximately the same size, conserve the relative size and position of the 12 exons associated with each gene, and at a nucleotide level the mouse and human EPO genes are 86% identical in the protein coding regions and 66% identical in the 3'-untranslated trailer regions. This strong conservation extends to the encoded proteins which show ~90% amino acid identity. Expression of the mEPO gene is restricted to tissues containing eosinophil progenitor cells (e.g., bone marrow and spleen), a pattern similar to the expression of another murine eosinophil granule protein, major basic protein.

Original languageEnglish (US)
Pages (from-to)285-294
Number of pages10
JournalJournal of Leukocyte Biology
Volume60
Issue number2
StatePublished - Aug 1996

Fingerprint

Eosinophil Peroxidase
Peroxidases
Organism Cloning
Genes
Eosinophil Granule Proteins
Complementary DNA
Bone Marrow
Genomic Library
3' Untranslated Regions
Gene Library
Eosinophils
Open Reading Frames
Exons
Proteins
Stem Cells
Spleen
Nucleotides

Keywords

  • Evolution
  • Gene organization
  • Granule proteins
  • Mouse eosinophils

ASJC Scopus subject areas

  • Cell Biology

Cite this

Cloning of the murine eosinophil peroxidase gene (mEPO) : Characterization of a conserved subgroup of mammalian hematopoietic peroxidases. / Horton, Margaret A.; Larson, Kirsten A.; Lee, James J.; Lee, Nancy A.

In: Journal of Leukocyte Biology, Vol. 60, No. 2, 08.1996, p. 285-294.

Research output: Contribution to journalArticle

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