Cloning of the mouse sodium iodide symporter

L. A. Pinke, D. S. Dean, E. R. Bergert, C. Spitzweg, C. M. Dutton, J. C. Morris

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

The iodide-concentrating ability of the thyroid gland is essential to the production of thyroid hormone. We report the nucleotide and amino acid sequence of the mouse sodium iodide symporter (mNIS), which mediates this activity within the thyroid gland. An open reading frame of 1,857 nucleotides codes for a protein of 618 amino acids with 95% identity to rat NIS and 84% identity to human NIS. Transient expression of the mNIS cDNA in Chinese hamster ovary (CHO) cells, a nonthyroid cell line, resulted in sodium-dependent, perchlorate-sensitive iodide uptake. Western blot analysis of membrane preparations of CHO cells transiently transfected with mNIS cDNA showed a band of 90 kd when probed with an antibody directed against rat NIS. mNIS will serve as an important reagent in determining the role of NIS in experimental thyroid diseases and for monitoring the immune response to in animal models of NIS-mediated gene therapy.

Original languageEnglish (US)
Pages (from-to)935-939
Number of pages5
JournalThyroid
Volume11
Issue number10
DOIs
StatePublished - Oct 2001

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology

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