Cloning of multiple copies of immunoglobulin variable kappa genes in cosmid vectors

Roberto Cattaneo, Jack Gorski, Bernard Mach

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

The possibility of cloning large segments of DNA in cosmid vectors offers distinct advantages, in particular for the study of multigene families. Large size fragments of mouse embryo DNA were successfully cloned in the cosmid pHC 79. Twelve recombinants hybridizing specifically to an immunoglobulin kappa chain variable region probe were identified. In 9 of these recombinants, the size of the insert ranges from 31 to 43 kilobases. Factors affecting the cloning efficiency of a complex mammalian genome in cosmids were studied. The stability of these recombinant cosmids and the preparation of recombinant cosmid DNA are also discussed.

Original languageEnglish (US)
Pages (from-to)2777-2790
Number of pages14
JournalNucleic acids research
Volume9
Issue number12
DOIs
StatePublished - Jun 25 1981

ASJC Scopus subject areas

  • Genetics

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