Abstract
The possibility of cloning large segments of DNA in cosmid vectors offers distinct advantages, in particular for the study of multigene families. Large size fragments of mouse embryo DNA were successfully cloned in the cosmid pHC 79. Twelve recombinants hybridizing specifically to an immunoglobulin kappa chain variable region probe were identified. In 9 of these recombinants, the size of the insert ranges from 31 to 43 kilobases. Factors affecting the cloning efficiency of a complex mammalian genome in cosmids were studied. The stability of these recombinant cosmids and the preparation of recombinant cosmid DNA are also discussed.
Original language | English (US) |
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Pages (from-to) | 2777-2790 |
Number of pages | 14 |
Journal | Nucleic acids research |
Volume | 9 |
Issue number | 12 |
DOIs | |
State | Published - Jun 25 1981 |
ASJC Scopus subject areas
- Genetics