Cloning of a putative human voltage-gated chloride channel (CIC-2) cDNA widely expressed in human tissues

Luis P. Cid, Charhzad Montrose-Rafizadeh, David I Smith, William B. Guggino, Garry R. Cutting

Research output: Contribution to journalArticle

77 Citations (Scopus)

Abstract

We have cloned a cDNA from the human epithelial cell line T84 whose predicted amino acid sequence shows 93.9% identity with rat CIC-2. Mapping by somatic cell hybrids and polymerase chain reaction localizes the gene corresponding to this cDNA to chromosome 3q26-qter. The major transcription start site assessed by RNA primer extension is 100 nt upstream of the putative translation initiation codon. Analysis of the 5′ flanking sequence revealed a high GC content and lack of common transcriptional elements such as TATA and CCAAT boxes. Northern blot analysis indicated wide organ distribution including tissues affected in cystic fibrosis (CF) and expression in an airway epithelial cell line derived from a CF patient. The high degree of sequence similarity and similar tissue distribution to rat CIC-2 suggests that this cDNA encodes the human CIC-2 voltage-gated chloride channel. Since this chloride channel is present in epithelial tissues it may be amenable to manipulation to circumvent the chloride secretion defect observed in CF.

Original languageEnglish (US)
Pages (from-to)407-413
Number of pages7
JournalHuman Molecular Genetics
Volume4
Issue number3
StatePublished - Mar 1995
Externally publishedYes

Fingerprint

Fibrosis
Chloride Channels
Cloning
CDNA
Cystic Fibrosis
Organism Cloning
Complementary DNA
Voltage
Tissue Distribution
Tissue
Rats
Cell
Electric potential
Epithelial Cells
Cell Line
TATA Box
Polymerase Chain Reaction
Initiator Codon
Hybrid Cells
Line

ASJC Scopus subject areas

  • Genetics
  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Public Health, Environmental and Occupational Health
  • Molecular Biology
  • Genetics(clinical)

Cite this

Cid, L. P., Montrose-Rafizadeh, C., Smith, D. I., Guggino, W. B., & Cutting, G. R. (1995). Cloning of a putative human voltage-gated chloride channel (CIC-2) cDNA widely expressed in human tissues. Human Molecular Genetics, 4(3), 407-413.

Cloning of a putative human voltage-gated chloride channel (CIC-2) cDNA widely expressed in human tissues. / Cid, Luis P.; Montrose-Rafizadeh, Charhzad; Smith, David I; Guggino, William B.; Cutting, Garry R.

In: Human Molecular Genetics, Vol. 4, No. 3, 03.1995, p. 407-413.

Research output: Contribution to journalArticle

Cid, LP, Montrose-Rafizadeh, C, Smith, DI, Guggino, WB & Cutting, GR 1995, 'Cloning of a putative human voltage-gated chloride channel (CIC-2) cDNA widely expressed in human tissues', Human Molecular Genetics, vol. 4, no. 3, pp. 407-413.
Cid, Luis P. ; Montrose-Rafizadeh, Charhzad ; Smith, David I ; Guggino, William B. ; Cutting, Garry R. / Cloning of a putative human voltage-gated chloride channel (CIC-2) cDNA widely expressed in human tissues. In: Human Molecular Genetics. 1995 ; Vol. 4, No. 3. pp. 407-413.
@article{2bf5a1a483ac4643aac72a5aa84cc901,
title = "Cloning of a putative human voltage-gated chloride channel (CIC-2) cDNA widely expressed in human tissues",
abstract = "We have cloned a cDNA from the human epithelial cell line T84 whose predicted amino acid sequence shows 93.9{\%} identity with rat CIC-2. Mapping by somatic cell hybrids and polymerase chain reaction localizes the gene corresponding to this cDNA to chromosome 3q26-qter. The major transcription start site assessed by RNA primer extension is 100 nt upstream of the putative translation initiation codon. Analysis of the 5′ flanking sequence revealed a high GC content and lack of common transcriptional elements such as TATA and CCAAT boxes. Northern blot analysis indicated wide organ distribution including tissues affected in cystic fibrosis (CF) and expression in an airway epithelial cell line derived from a CF patient. The high degree of sequence similarity and similar tissue distribution to rat CIC-2 suggests that this cDNA encodes the human CIC-2 voltage-gated chloride channel. Since this chloride channel is present in epithelial tissues it may be amenable to manipulation to circumvent the chloride secretion defect observed in CF.",
author = "Cid, {Luis P.} and Charhzad Montrose-Rafizadeh and Smith, {David I} and Guggino, {William B.} and Cutting, {Garry R.}",
year = "1995",
month = "3",
language = "English (US)",
volume = "4",
pages = "407--413",
journal = "Human Molecular Genetics",
issn = "0964-6906",
publisher = "Oxford University Press",
number = "3",

}

TY - JOUR

T1 - Cloning of a putative human voltage-gated chloride channel (CIC-2) cDNA widely expressed in human tissues

AU - Cid, Luis P.

AU - Montrose-Rafizadeh, Charhzad

AU - Smith, David I

AU - Guggino, William B.

AU - Cutting, Garry R.

PY - 1995/3

Y1 - 1995/3

N2 - We have cloned a cDNA from the human epithelial cell line T84 whose predicted amino acid sequence shows 93.9% identity with rat CIC-2. Mapping by somatic cell hybrids and polymerase chain reaction localizes the gene corresponding to this cDNA to chromosome 3q26-qter. The major transcription start site assessed by RNA primer extension is 100 nt upstream of the putative translation initiation codon. Analysis of the 5′ flanking sequence revealed a high GC content and lack of common transcriptional elements such as TATA and CCAAT boxes. Northern blot analysis indicated wide organ distribution including tissues affected in cystic fibrosis (CF) and expression in an airway epithelial cell line derived from a CF patient. The high degree of sequence similarity and similar tissue distribution to rat CIC-2 suggests that this cDNA encodes the human CIC-2 voltage-gated chloride channel. Since this chloride channel is present in epithelial tissues it may be amenable to manipulation to circumvent the chloride secretion defect observed in CF.

AB - We have cloned a cDNA from the human epithelial cell line T84 whose predicted amino acid sequence shows 93.9% identity with rat CIC-2. Mapping by somatic cell hybrids and polymerase chain reaction localizes the gene corresponding to this cDNA to chromosome 3q26-qter. The major transcription start site assessed by RNA primer extension is 100 nt upstream of the putative translation initiation codon. Analysis of the 5′ flanking sequence revealed a high GC content and lack of common transcriptional elements such as TATA and CCAAT boxes. Northern blot analysis indicated wide organ distribution including tissues affected in cystic fibrosis (CF) and expression in an airway epithelial cell line derived from a CF patient. The high degree of sequence similarity and similar tissue distribution to rat CIC-2 suggests that this cDNA encodes the human CIC-2 voltage-gated chloride channel. Since this chloride channel is present in epithelial tissues it may be amenable to manipulation to circumvent the chloride secretion defect observed in CF.

UR - http://www.scopus.com/inward/record.url?scp=0028915816&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028915816&partnerID=8YFLogxK

M3 - Article

C2 - 7795595

AN - SCOPUS:0028915816

VL - 4

SP - 407

EP - 413

JO - Human Molecular Genetics

JF - Human Molecular Genetics

SN - 0964-6906

IS - 3

ER -