Cloning of a human ortholog (RPH3AL) of (RNO)Rph3al from a candidate 17p13.3 medulloblastoma tumor suppressor locus

Justin S. Smith, Issei Tachibana, Cory Allen, Sharon A. Chiappa, Hyun K. Lee, Bryan McIver, Robert B. Jenkins, Corey Raffel

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

Allelic loss of 17p13.3 is observed in approximately 40% of medulloblastomas, suggesting the presence of a tumor suppressor gene in this region. Deletion mapping has defined a region of common loss flanking the telomeric marker D17S34, and a recent report delineated a 9-kb homozygous deletion within the D17S34 locus in one such tumor. Using cDNA selection, we have identified a transcript spanning this deletion, designated (HSA)RPH3AL (rabphillin-3A-like), based on its 77% overall amino acid identity with a recently cloned rat gene, (RNO)Rph3al (originally termed Noc2), a gene putatively involved in regulated endocrine exocytosis through its interactions with the cytoskeleton. We determined the exon-intron boundaries of RPH3AL and screened the coding region for mutations by direct sequencing in DNA extracted from 33 tumor samples with allelic loss of 17p13, including 10 medulloblastoma, 14 follicular thyroid cancer (FTC), and 9 ovarian cancer specimens. No mutations were identified. Thus, despite its location in a homozygously deleted 17p13.3 locus, it is unlikely that RPH3AL is a gene involved in the oncogenesis of medulloblastoma, FTC, or ovarian cancer.

Original languageEnglish (US)
Pages (from-to)97-101
Number of pages5
JournalGenomics
Volume59
Issue number1
DOIs
StatePublished - Jul 1 1999

ASJC Scopus subject areas

  • Genetics

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    Smith, J. S., Tachibana, I., Allen, C., Chiappa, S. A., Lee, H. K., McIver, B., Jenkins, R. B., & Raffel, C. (1999). Cloning of a human ortholog (RPH3AL) of (RNO)Rph3al from a candidate 17p13.3 medulloblastoma tumor suppressor locus. Genomics, 59(1), 97-101. https://doi.org/10.1006/geno.1999.5864