Cloning, function, and localization of human, canine, and Drosophila ZIP10 (SLC39A10), a Zn2+ transporter

Greg M. Landry, Eva Furrow, Heather L. Holmes, Taku Hirata, Akira Kato, Paige Williams, Käri Strohmaier, Chris J.R. Gallo, Minhwang Chang, Mukesh Pandey, Huailei Jiang, Aditya Bansal, Marie Christine Franz, Nicolas Montalbetti, Mariam P Alexander, Pablo Cabrero, Julian A.T. Dow, Timothy R DeGrado, Michael F Romero

Research output: Contribution to journalArticle

Abstract

Zinc (Zn2+) is the second most abundant trace element, but is considered a micronutrient, as it is a cofactor for many enzymes and transcription factors. Whereas Zn2+ deficiency can cause cognitive immune or metabolic dysfunction and infertility, excess Zn2+ is nephrotoxic. As for other ions and solutes, Zn2+ is moved into and out of cells by specific membrane transporters: ZnT, Zip, and NRAMP/DMT proteins. ZIP10 is reported to be localized at the apical membrane of renal proximal tubules in rats, where it is believed to play a role in Zn2+ import. Renal regulation of Zn2+ is of particular interest in light of growing evidence that Zn2+ may play a role in kidney stone formation. The objective of this study was to show that ZIP10 homologs transport Zn2+, as well as ZIP10, kidney localization across species. We cloned ZIP10 from dog, human, and Drosophila ( CG10006), tested clones for Zn2+ uptake in Xenopus oocytes and localized the protein in renal structures. CG10006, rather than foi (fear-of-intimacy, CG6817) is the primary ZIP10 homolog found in Drosophila Malpighian tubules. The ZIP10 antibody recognizes recombinant dog, human, and Drosophila ZIP10 proteins. Immunohistochemistry reveals that ZIP10 in higher mammals is found not only in the proximal tubule, but also in the collecting duct system. These ZIP10 proteins show Zn2+ transport. Together, these studies reveal ZIP10 kidney localization, a role in renal Zn2+ transport, and indicates that CG10006 is a Drosophila homolog of ZIP10.

Original languageEnglish (US)
Pages (from-to)F263-F273
JournalAmerican journal of physiology. Renal physiology
Volume316
Issue number2
DOIs
StatePublished - Feb 1 2019

Fingerprint

Drosophila
Canidae
Organism Cloning
Kidney
Malpighian Tubules
Dogs
Drosophila Proteins
Proximal Kidney Tubule
Proteins
Kidney Calculi
Membrane Transport Proteins
Micronutrients
Coenzymes
Trace Elements
Xenopus
Infertility
Fear
Oocytes
Zinc
Mammals

Keywords

  • oocyte expression
  • immunohistochemistry
  • kidney
  • PET isotope
  • Slc39a10

ASJC Scopus subject areas

  • Physiology
  • Urology

Cite this

Cloning, function, and localization of human, canine, and Drosophila ZIP10 (SLC39A10), a Zn2+ transporter. / Landry, Greg M.; Furrow, Eva; Holmes, Heather L.; Hirata, Taku; Kato, Akira; Williams, Paige; Strohmaier, Käri; Gallo, Chris J.R.; Chang, Minhwang; Pandey, Mukesh; Jiang, Huailei; Bansal, Aditya; Franz, Marie Christine; Montalbetti, Nicolas; Alexander, Mariam P; Cabrero, Pablo; Dow, Julian A.T.; DeGrado, Timothy R; Romero, Michael F.

In: American journal of physiology. Renal physiology, Vol. 316, No. 2, 01.02.2019, p. F263-F273.

Research output: Contribution to journalArticle

Landry, GM, Furrow, E, Holmes, HL, Hirata, T, Kato, A, Williams, P, Strohmaier, K, Gallo, CJR, Chang, M, Pandey, M, Jiang, H, Bansal, A, Franz, MC, Montalbetti, N, Alexander, MP, Cabrero, P, Dow, JAT, DeGrado, TR & Romero, MF 2019, 'Cloning, function, and localization of human, canine, and Drosophila ZIP10 (SLC39A10), a Zn2+ transporter', American journal of physiology. Renal physiology, vol. 316, no. 2, pp. F263-F273. https://doi.org/10.1152/ajprenal.00573.2017
Landry, Greg M. ; Furrow, Eva ; Holmes, Heather L. ; Hirata, Taku ; Kato, Akira ; Williams, Paige ; Strohmaier, Käri ; Gallo, Chris J.R. ; Chang, Minhwang ; Pandey, Mukesh ; Jiang, Huailei ; Bansal, Aditya ; Franz, Marie Christine ; Montalbetti, Nicolas ; Alexander, Mariam P ; Cabrero, Pablo ; Dow, Julian A.T. ; DeGrado, Timothy R ; Romero, Michael F. / Cloning, function, and localization of human, canine, and Drosophila ZIP10 (SLC39A10), a Zn2+ transporter. In: American journal of physiology. Renal physiology. 2019 ; Vol. 316, No. 2. pp. F263-F273.
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