Cloning, expression and characterization of the human NOB1 gene

Yue Zhang, Jun Ni, Guangjin Zhou, Jian Yuan, Weihua Ren, Yuxi Shan, Wenwen Tang, Long Yu, Shouyuan Zhao

Research output: Contribution to journalArticle

34 Scopus citations

Abstract

The yeast Nob1p (Nin one binding protein) gene is required for proteasome function and RNA metabolism. We report here the cloning and characterization of the human orthologue NOB1 gene and its products. The human NOB1 gene is composed of nine exons and eight introns and is localized on human chromosome 16q22.1. The NOB1 cDNA is 1749 bp long and contains a putative open reading frame of 1239 bp. The predicted NOB1 protein comprises a PIN (PilT amino terminus) domain and a zinc ribbon domain. Western blot analysis showed that the molecular weight of NOB1 is about 50 KDa. RT-PCR analysis of mRNA from human adult tissues showed that NOB1 is expressed mainly in liver, lung and spleen. Expression of NOB1 in mammalian culture cells indicated that the NOB1 protein is mainly localized in the nucleus. Our data provides important information for further study of the function of the NOB1 gene and its products.

Original languageEnglish (US)
Pages (from-to)185-189
Number of pages5
JournalMolecular Biology Reports
Volume32
Issue number3
DOIs
StatePublished - Sep 1 2005

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Keywords

  • NOB1
  • PIN domain
  • Subcellular localization
  • Tissue expression pattern
  • Zinc ribbon domain

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Zhang, Y., Ni, J., Zhou, G., Yuan, J., Ren, W., Shan, Y., Tang, W., Yu, L., & Zhao, S. (2005). Cloning, expression and characterization of the human NOB1 gene. Molecular Biology Reports, 32(3), 185-189. https://doi.org/10.1007/s11033-005-3141-7