TY - JOUR
T1 - Cloning and characterization of the T(15;17) translocation breakpoint region in acute promyelocytic leukemia
AU - Lemons, Richard S.
AU - Eilender, David
AU - Waldmann, Richard A.
AU - Rebentisch, Matt
AU - Frej, Ann‐Kristin ‐K
AU - Ledbetter, David H.
AU - Willman, Cheryl
AU - McConnell, Thomas
AU - O'Connell, Peter
PY - 1990/7
Y1 - 1990/7
N2 - A reciprocal chromosomal translocation, t(15;17)(q22;q11.2‐12), is characteristic of acute promyelocytic leukemia (APL) of French‐American‐British (FAB) subtype M3, and is not associated with any other human malignancy. The non‐random pattern of the APL translocations suggests that specific genes on chromosomes 15 and 17 are somehow altered or deregulated as a consequence of the rearrangement. Translocation breakpoints in APL patients provide physical landmarks that suggest an approach to isolating the APL gene(s). Genetic and physical maps constructed for the APL breakpoint region on chromosome 17 have indicated that two fully‐linked DNA markers, defining loci for THRA1 and D17S80, map to opposite sides of an APL breakpoint yet reside on a common 350‐kb Cla1 fragment. Cosmid‐walking experiments to clone this APL breakpoint have revealed a 38‐kilobase deletion on chromosome 17. Studies in additional APL patients have shown that the breakpoint region on chromosome 17 spans at least 80 kilobases.
AB - A reciprocal chromosomal translocation, t(15;17)(q22;q11.2‐12), is characteristic of acute promyelocytic leukemia (APL) of French‐American‐British (FAB) subtype M3, and is not associated with any other human malignancy. The non‐random pattern of the APL translocations suggests that specific genes on chromosomes 15 and 17 are somehow altered or deregulated as a consequence of the rearrangement. Translocation breakpoints in APL patients provide physical landmarks that suggest an approach to isolating the APL gene(s). Genetic and physical maps constructed for the APL breakpoint region on chromosome 17 have indicated that two fully‐linked DNA markers, defining loci for THRA1 and D17S80, map to opposite sides of an APL breakpoint yet reside on a common 350‐kb Cla1 fragment. Cosmid‐walking experiments to clone this APL breakpoint have revealed a 38‐kilobase deletion on chromosome 17. Studies in additional APL patients have shown that the breakpoint region on chromosome 17 spans at least 80 kilobases.
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U2 - 10.1002/gcc.2870020202
DO - 10.1002/gcc.2870020202
M3 - Article
C2 - 2278973
AN - SCOPUS:0024995020
SN - 1045-2257
VL - 2
SP - 79
EP - 87
JO - Genes, Chromosomes and Cancer
JF - Genes, Chromosomes and Cancer
IS - 2
ER -