Cloning and characterization of the human gene DERP6, which activates transcriptional activities of p53

Jian Yuan; Wenwen Tang; Kuntian Luo; Xinya Chen; Xiuting Gu; Bo Wan; Long Yu

doi:10.1007/s11033-006-6273-5

Cloning and characterization of the human gene DERP6, which activates transcriptional activities of p53

Jian Yuan, Wenwen Tang, Kuntian Luo, Xinya Chen, Xiuting Gu, Bo Wan, Long Yu

Pharmacology

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

We report here the cloning and characterization of the human gene DERP6, isolated from human testis cDNA library, and mapped to 17p13.1 by searching the UCSC genomic database. The DERP6 cDNA consists of 1486 nucleotides and has a 316-amino acids open reading frame. The Northern hybridization analysis showed that DERP6 was ubiquitously expressed in all the 16 adult tissues, especially highly expressed in heart, brain, liver, skeletal muscle and testis. DERP6 protein was located in cytoplasm when overexpressed in cultured cells. Reporter gene assays showed that overexpression of DERP6 in cells activated the transcriptional activities of p53. These results indicate that the human gene DERP6 may be involved in p53-mediated gene transcription.

Original language	English (US)
Pages (from-to)	151-158
Number of pages	8
Journal	Molecular Biology Reports
Volume	33
Issue number	3
DOIs	https://doi.org/10.1007/s11033-006-6273-5
State	Published - Sep 2006

Keywords

DERP6
Transcription
p53

ASJC Scopus subject areas

Molecular Biology
Genetics

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10.1007/s11033-006-6273-5

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title = "Cloning and characterization of the human gene DERP6, which activates transcriptional activities of p53",

abstract = "We report here the cloning and characterization of the human gene DERP6, isolated from human testis cDNA library, and mapped to 17p13.1 by searching the UCSC genomic database. The DERP6 cDNA consists of 1486 nucleotides and has a 316-amino acids open reading frame. The Northern hybridization analysis showed that DERP6 was ubiquitously expressed in all the 16 adult tissues, especially highly expressed in heart, brain, liver, skeletal muscle and testis. DERP6 protein was located in cytoplasm when overexpressed in cultured cells. Reporter gene assays showed that overexpression of DERP6 in cells activated the transcriptional activities of p53. These results indicate that the human gene DERP6 may be involved in p53-mediated gene transcription.",

keywords = "DERP6, Transcription, p53",

author = "Jian Yuan and Wenwen Tang and Kuntian Luo and Xinya Chen and Xiuting Gu and Bo Wan and Long Yu",

note = "Funding Information: This work was supported by the National 973 Program, 863 High Technology Program and the National Natural Science Foundation of China.",

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AU - Yuan, Jian

AU - Tang, Wenwen

AU - Luo, Kuntian

AU - Chen, Xinya

AU - Gu, Xiuting

AU - Wan, Bo

AU - Yu, Long

N1 - Funding Information: This work was supported by the National 973 Program, 863 High Technology Program and the National Natural Science Foundation of China.

PY - 2006/9

Y1 - 2006/9

N2 - We report here the cloning and characterization of the human gene DERP6, isolated from human testis cDNA library, and mapped to 17p13.1 by searching the UCSC genomic database. The DERP6 cDNA consists of 1486 nucleotides and has a 316-amino acids open reading frame. The Northern hybridization analysis showed that DERP6 was ubiquitously expressed in all the 16 adult tissues, especially highly expressed in heart, brain, liver, skeletal muscle and testis. DERP6 protein was located in cytoplasm when overexpressed in cultured cells. Reporter gene assays showed that overexpression of DERP6 in cells activated the transcriptional activities of p53. These results indicate that the human gene DERP6 may be involved in p53-mediated gene transcription.

AB - We report here the cloning and characterization of the human gene DERP6, isolated from human testis cDNA library, and mapped to 17p13.1 by searching the UCSC genomic database. The DERP6 cDNA consists of 1486 nucleotides and has a 316-amino acids open reading frame. The Northern hybridization analysis showed that DERP6 was ubiquitously expressed in all the 16 adult tissues, especially highly expressed in heart, brain, liver, skeletal muscle and testis. DERP6 protein was located in cytoplasm when overexpressed in cultured cells. Reporter gene assays showed that overexpression of DERP6 in cells activated the transcriptional activities of p53. These results indicate that the human gene DERP6 may be involved in p53-mediated gene transcription.

KW - DERP6

KW - Transcription

KW - p53

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Cloning and characterization of the human gene DERP6, which activates transcriptional activities of p53

Abstract

Keywords

ASJC Scopus subject areas

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