TY - JOUR
T1 - Cloning and characterization of hCTF18, hCTF8, and hDCC1
T2 - Human homologs of an Saccharomyces cerevisiae complex involved in sister chromatid cohesion establishment
AU - Merkle, Carolin J.
AU - Karnitz, Larry M.
AU - Henry-Sánchez, John T.
AU - Chen, Junjie
N1 - Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2003/8/8
Y1 - 2003/8/8
N2 - A growing body of evidence suggests that establishment of sister chromatid cohesion is dependent on replication fork passage over a precohesion area. In Saccharomyces cerevisiae, this process involves an alternative replication factor C (RFC) complex that contains the four small RFC subunits as well as CTF18, CTF8, and DCC1. Here, we show that an evolutionarily conserved homologous complex exists in the nucleus of human cells. We demonstrate that hCTF18, hCTF8, and hDCC1 interact with each other as well as with the p38 subunit of RFC. This alternative RFC-containing complex interacts with proliferating cell nuclear antigen but not with the Rad9/Rad1/Hus1 complex, a proliferating cell nuclear antigen-like clamp involved in the DNA damage response. hCTF18 preferentially binds chromatin during S phase, suggesting a role during replication. Our data provide evidence for the existence of an alternative RFC complex with a probable role in mammalian sister chromatid cohesion establishment.
AB - A growing body of evidence suggests that establishment of sister chromatid cohesion is dependent on replication fork passage over a precohesion area. In Saccharomyces cerevisiae, this process involves an alternative replication factor C (RFC) complex that contains the four small RFC subunits as well as CTF18, CTF8, and DCC1. Here, we show that an evolutionarily conserved homologous complex exists in the nucleus of human cells. We demonstrate that hCTF18, hCTF8, and hDCC1 interact with each other as well as with the p38 subunit of RFC. This alternative RFC-containing complex interacts with proliferating cell nuclear antigen but not with the Rad9/Rad1/Hus1 complex, a proliferating cell nuclear antigen-like clamp involved in the DNA damage response. hCTF18 preferentially binds chromatin during S phase, suggesting a role during replication. Our data provide evidence for the existence of an alternative RFC complex with a probable role in mammalian sister chromatid cohesion establishment.
UR - http://www.scopus.com/inward/record.url?scp=0041529523&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0041529523&partnerID=8YFLogxK
U2 - 10.1074/jbc.M211591200
DO - 10.1074/jbc.M211591200
M3 - Article
C2 - 12766176
AN - SCOPUS:0041529523
SN - 0021-9258
VL - 278
SP - 30051
EP - 30056
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 32
ER -