Cloning and characterization of a human electrogenic Na+-HCO3/- cotransporter isoform (hhNBC)

Inyeong Choi, Michael F Romero, Nassirah Khandoudi, Antoine Bril, Walter F. Boron

Research output: Contribution to journalArticle

128 Citations (Scopus)

Abstract

Our group recently cloned the electrogenic Na+-HCO3/- cotransporter (NBC) from salamander kidney and later from mammalian kidney. Here we report cloning an NBC isoform (hhNBC) from a human heart cDNA library. hhNBC is identical to human renal NBC (hkNBC), except for the amino terminus, where the first 85 amino acids in hhNBC replace the first 41 amino acids of hkNBC. About 50% of the amino acid residues in this unique amino terminus are charged, compared with ~22% for the corresponding 41 residues in hkNBC. Northern blot analysis, with the use of the unique 5' fragment of hhNBC as a probe, shows strong expression in pancreas and expression in heart and brain, although at much lower levels. In Xenopus oocytes expressing hhNBC, adding 1.5% CO2/10 mM HCO3/- hyperpolarizes the membrane and causes a rapid fall in intracellular pH (pH(i)), followed by a phi recovery. Subsequent removal of Na+ causes a depolarization and a reduced rate of phi recovery. Removal of Cl- from the bath does not affect the ph(i) recovery. The stilbene derivative DIDS (200 μM) greatly reduces the hyperpolarization caused by adding CO2/HCO3/-. In oocytes expressing hkNBC, the effects of adding CO2/HCO3/- and then removing Na+ were similar to those observed in oocytes expressing hhNBC. We conclude that hhNBC is an electrogenic Na+HCO3/- cotransporter and that hkNBC is also electrogenic.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Cell Physiology
Volume276
Issue number3 45-3
StatePublished - 1999
Externally publishedYes

Fingerprint

Cloning
Oocytes
Organism Cloning
Protein Isoforms
Kidney
Amino Acids
Recovery
4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid
Urodela
Stilbenes
Depolarization
Xenopus
Baths
Gene Library
Northern Blotting
Pancreas
Brain
Derivatives
Membranes

Keywords

  • Acid extruder
  • Bicarbonate
  • Intracellular pH
  • Oocytes

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology
  • Physiology (medical)

Cite this

Cloning and characterization of a human electrogenic Na+-HCO3/- cotransporter isoform (hhNBC). / Choi, Inyeong; Romero, Michael F; Khandoudi, Nassirah; Bril, Antoine; Boron, Walter F.

In: American Journal of Physiology - Cell Physiology, Vol. 276, No. 3 45-3, 1999.

Research output: Contribution to journalArticle

Choi, Inyeong ; Romero, Michael F ; Khandoudi, Nassirah ; Bril, Antoine ; Boron, Walter F. / Cloning and characterization of a human electrogenic Na+-HCO3/- cotransporter isoform (hhNBC). In: American Journal of Physiology - Cell Physiology. 1999 ; Vol. 276, No. 3 45-3.
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